Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor
Abstract
:1. Introduction
2. Results and Discussion
2.1. Aβ Immobilization
2.2. Real-Time Determination of Aβ1–28 Binding Zn2+ and Cu2+ by SPR
2.3. Real-Time Determination of Aβ1–42 Binding to Zn2+ and Cu2+ by SPR
3. Experimental Section
3.1. Chemicals
3.2. Preparation of Fresh Aβ Solution
3.3. Gold Film Preparation
3.4. Aβ Peptide Immobilization
3.5. SPR Apparatus
4. Conclusions
Acknowledgments
References
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Yao, F.; Zhang, R.; Tian, H.; Li, X. Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor. Int. J. Mol. Sci. 2012, 13, 11832-11843. https://doi.org/10.3390/ijms130911832
Yao F, Zhang R, Tian H, Li X. Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor. International Journal of Molecular Sciences. 2012; 13(9):11832-11843. https://doi.org/10.3390/ijms130911832
Chicago/Turabian StyleYao, Fujun, Ruiping Zhang, He Tian, and Xiangjun Li. 2012. "Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor" International Journal of Molecular Sciences 13, no. 9: 11832-11843. https://doi.org/10.3390/ijms130911832