Neuritogenic Monoglyceride Derived from the Constituent of a Marine Fish for Activating the PI3K/ERK/CREB Signalling Pathways in PC12 Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Structural Elucidation of 1-O-(Myristoyl) Glycerol
2.2. NGF Mimic Activity of 1-O-(Myristoyl) Glycerol
2.3. Synthesis of 1-O-(myristoyl) Glycerol Derivatives
2.4. Mechanism of Action of 1-O-(Stearoyl) Glycerol
3. Experimental Section
3.1. Extraction and Isolation
3.2. Chemical Analysis
3.3. Synthesis Method
3.4. Bioassay of NGF Mimicking Activity in PC12 Cells
3.5. MTT Assay
3.6. Inhibitor Test
3.7. Western Blot Analysis
4. Conclusions
Acknowledgments
Abbreviation
AD | Alzheimer’s disease |
BBB | blood brain barrier |
CREB | cAMP responsive element-binding protein |
DMEM | dulbecco’s modified eagle medium |
DMSO | dimethyl sulfoxide |
ERK | extracellular regulated protein kinases |
MEK | mitogen-activated kinase/ERK kinase |
MG | 1-O-(myristoyl) glycerol |
MTT | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide |
NGF | nerve growth factor |
NMDA | N-methyl-d-aspartic acid |
PI3K | phosphatidylinositol-3 kinase |
SAR | structure-activity relationship |
SG | 1-O-(stearoyl) glycerol |
Conflicts of Interest
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Yang, W.; Luo, Y.; Tang, R.; Zhang, H.; Ye, Y.; Xiang, L.; Qi, J. Neuritogenic Monoglyceride Derived from the Constituent of a Marine Fish for Activating the PI3K/ERK/CREB Signalling Pathways in PC12 Cells. Int. J. Mol. Sci. 2013, 14, 24200-24210. https://doi.org/10.3390/ijms141224200
Yang W, Luo Y, Tang R, Zhang H, Ye Y, Xiang L, Qi J. Neuritogenic Monoglyceride Derived from the Constituent of a Marine Fish for Activating the PI3K/ERK/CREB Signalling Pathways in PC12 Cells. International Journal of Molecular Sciences. 2013; 14(12):24200-24210. https://doi.org/10.3390/ijms141224200
Chicago/Turabian StyleYang, Wei, Yan Luo, Ruiqi Tang, Hui Zhang, Ying Ye, Lan Xiang, and Jianhua Qi. 2013. "Neuritogenic Monoglyceride Derived from the Constituent of a Marine Fish for Activating the PI3K/ERK/CREB Signalling Pathways in PC12 Cells" International Journal of Molecular Sciences 14, no. 12: 24200-24210. https://doi.org/10.3390/ijms141224200