CXCL10 Decreases GP73 Expression in Hepatoma Cells at the Early Stage of Hepatitis C Virus (HCV) Infection
Abstract
:1. Introduction
2. Results
2.1. GP73 mRNA Level Is Down-Regulated in HCV Infected Cells
2.2. CXCL10 Is Involved in the Down-Regulation of GP73
2.3. GP73 Is Up-Regulated in Cells with Cytopathic Effect Induced by HCV Infection
2.4. GP73 Had No Impact on Proliferation of Vascular Endothelial Cells
3. Discussion
4. Materials and Methods
4.1. Cells and Plasmids Construction
4.2. Viral RNA in Vitro Transcriptions
4.3. Real Time PCR
4.4. Cytokine Stimulation
4.5. Immunofluorescence Assay
4.6. Cell Proliferation Assay
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Abbreviations
GP73 | Golgi protein 73 |
HCC | Hepatocellular carcinoma |
HCV | Hepatitis C virus |
GOLPH2 | Golgi Phosphoprotein 2 |
GOLM1 | Golgi membrane protein 1 |
SNPs | Single-nucleotide polymorphisms |
AD | Alzheimer’s disease |
AFP | Alpha-Fetoprotein |
GPC3 | Glypican-3 |
DCP | Des-γ-Carboxy Prothrombin |
CXCL10 | C-X-C motif chemokine 10 |
CPE | Cytopathic effect |
HUVEC | Human umbilical vein endothelial cells |
IFN | Interferon |
TNF | Tumor necrosis factor |
IL-6 | Interleukin-6 |
OSM | oncostatin M |
Conflicts of Interests
References
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Gene | Primer sequences (5′-3′) |
---|---|
GP73 | Forward:CATAGAAGCTTCTCCAGACACGGATCATGGAGCTGGAAGGC |
Reverse:GAGAGGGATCCTCAGAGTGTATGATTCCGCTTTTCACGCTG | |
CXCL10 | Forward:CATAGAAGCTTCTCGAGATGCTCCTGGCTGTTTTGTACTGCCTGCTGTGGAGTTTCCAGACCTCCGCTGGCCATTTCCCTAGAATGAATCAAACTGCCATTCTG |
Reverse:GAGAGGGATCCAACATAGCACCTCAGTAGAGC |
Genes | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|
HCV | CTTCACGCAGAAAGCGTCTA | CAAGCACCCTATCAGGCAGT |
GP73 | TTGGTAACAGCAAGTCCCAGACA | ACCACCTGGATCTCATTGGTTTC |
CXCL10 | AGGAACCTCCAGTCTCAGCA | CAAAATTGGCTTGCAGGAAT |
GAPDH | TGGGCTACACTGAGCACCAG | AAGTGGTCGTTGAGGGCAAT |
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Liu, Y.; Zou, Z.; Zhu, B.; Hu, Z.; Zeng, P. CXCL10 Decreases GP73 Expression in Hepatoma Cells at the Early Stage of Hepatitis C Virus (HCV) Infection. Int. J. Mol. Sci. 2013, 14, 24230-24241. https://doi.org/10.3390/ijms141224230
Liu Y, Zou Z, Zhu B, Hu Z, Zeng P. CXCL10 Decreases GP73 Expression in Hepatoma Cells at the Early Stage of Hepatitis C Virus (HCV) Infection. International Journal of Molecular Sciences. 2013; 14(12):24230-24241. https://doi.org/10.3390/ijms141224230
Chicago/Turabian StyleLiu, Yuan, Ziying Zou, Bing Zhu, Zonghai Hu, and Ping Zeng. 2013. "CXCL10 Decreases GP73 Expression in Hepatoma Cells at the Early Stage of Hepatitis C Virus (HCV) Infection" International Journal of Molecular Sciences 14, no. 12: 24230-24241. https://doi.org/10.3390/ijms141224230