Characterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of Marine Cellulophaga lytica Strain N5-2
2.2. Purification of κ-Carrageenase
2.3. Properties of the κ-Carragcenase
2.4. Analysis of Degradation Products
3. Materials and Methods
3.1. Microorganism
3.2. Chemicals
3.3. κ-Carrageenase Assay
3.4. Purification of κ-Carrageenase
3.5. Zymography Experiment
3.6. Dependence and Stability of the Enzyme Activity on Temperature and pH
3.7. Kinetic Studies
3.8. Analysis of Degradation Products
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Step | Volume (mL) | Total protein a (mg) | Total activity b (units) | Specific activity (units/mg) | Folds | Recovery (%) |
---|---|---|---|---|---|---|
Cell-free medium | 1000 | 56.5 | 1648 | 29 | 1 | 100 |
40% and 80% (NH4)2SO4 precipitate | 80 | 11 | 1145 | 104 | 4 | 69.5 |
SephadexG-200 gel-filtration | 5 | 0.86 | 580 | 675 | 23 | 35.2 |
SephadexG-75 gel-filtration | 2 | 0.235 | 285 | 1170 | 40 | 17.3 |
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Yao, Z.; Wang, F.; Gao, Z.; Jin, L.; Wu, H. Characterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products. Int. J. Mol. Sci. 2013, 14, 24592-24602. https://doi.org/10.3390/ijms141224592
Yao Z, Wang F, Gao Z, Jin L, Wu H. Characterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products. International Journal of Molecular Sciences. 2013; 14(12):24592-24602. https://doi.org/10.3390/ijms141224592
Chicago/Turabian StyleYao, Ziang, Feifei Wang, Zheng Gao, Liming Jin, and Haige Wu. 2013. "Characterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products" International Journal of Molecular Sciences 14, no. 12: 24592-24602. https://doi.org/10.3390/ijms141224592