Nuclear Lipid Microdomain as Place of Interaction between Sphingomyelin and DNA during Liver Regeneration
Abstract
:1. Introduction
2. Results and Discussion
2.1. Nuclear Lipid Microdomains during Liver Regeneration
2.2. Sphingomyelin in Nuclear Lipid Microdomain during Liver Regeneration
2.3. Effect of Trifluoroperazine Treatment
3. Experimental Section
3.1. Materials
3.2. Animals
3.3. Liver Regeneration
3.4. Microsomes and Subnuclear Fractions Purification
3.5. DNA Synthesis
3.6. Lipid Analysis
3.7. Sphingomyelinase Treatment
3.8. Neutral-Sphingomyelinase and Sphingomyelin-Synthase Activity
3.9. Electrophoresis and Western Blot Analysis
4. Conclusions
Acknowledgements
Conflict of Interest
References
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Time | Nuclear membrane | Nuclear matrix | Chromatin | Nuclear microdomain |
---|---|---|---|---|
0 h | 9.3 ± 0.7 | 1.6 ± 0.3 | 0.9 ± 0.1 | 15.5 ± 0.3 |
6 h | 12.8 ± 0.5 * | 3.7 ± 0.6 * | 0.7 ± 0.2 | 14.1 ± 0.2 |
12 h | 12.1 ± 1.3 * | 6.5 ± 0.5 * | 2.2 ± 0.1 * | 38.2 ± 0.7 * |
18 h | 21.3 ± 0.7 * | 7.7 ± 0.2 * | 0.9 ± 0.1 | 15.7 ± 0.1 |
24 h | 9.3 ± 0.3 | 6.8 ± 0.5 * | 1.2 ± 0.2 | 19.5 ± 0.2 * |
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Albi, E.; Lazzarini, A.; Lazzarini, R.; Floridi, A.; Damaskopoulou, E.; Curcio, F.; Cataldi, S. Nuclear Lipid Microdomain as Place of Interaction between Sphingomyelin and DNA during Liver Regeneration. Int. J. Mol. Sci. 2013, 14, 6529-6541. https://doi.org/10.3390/ijms14046529
Albi E, Lazzarini A, Lazzarini R, Floridi A, Damaskopoulou E, Curcio F, Cataldi S. Nuclear Lipid Microdomain as Place of Interaction between Sphingomyelin and DNA during Liver Regeneration. International Journal of Molecular Sciences. 2013; 14(4):6529-6541. https://doi.org/10.3390/ijms14046529
Chicago/Turabian StyleAlbi, Elisabetta, Andrea Lazzarini, Remo Lazzarini, Alessandro Floridi, Eleni Damaskopoulou, Francesco Curcio, and Samuela Cataldi. 2013. "Nuclear Lipid Microdomain as Place of Interaction between Sphingomyelin and DNA during Liver Regeneration" International Journal of Molecular Sciences 14, no. 4: 6529-6541. https://doi.org/10.3390/ijms14046529