Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF)
Abstract
:1. Introduction
2. Results and Discussion
2.1. A Preliminary Analysis of the 45 Samples
2.2. The Verification of Preliminary Results
3. Experimental Section
3.1. Patient Selection, Treatment and Sample Collection
3.1.1. Patient Selection and Treatment
3.1.2. Sample Collection
3.2. Raman Analysis
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Relative concentration (C′) | Range of C′ | Embryo developmental capacity | Number of sample |
---|---|---|---|
Sodium pyruvate/albumin | <0.012 | + | 4, 6, 7, 11, 12, 14, 16, 18, 21, 23, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 41, 44, 45 |
>0.012 | — | 1, 3, 5, 8, 9, 10, 13, 17, 22, 24, 25, 42 | |
Phenylalanine/albumin | ≥0.00085 | + | 12, 14, 21, 23, 27, 28, 29, 31, 33, 34, 35, 36, 37, 38, 41, 44 |
≤0.00085 | — | 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 16, 17, 18, 22, 24, 25, 26, 30, 32, 42, 45 |
Age (years) | Endometrial thickness (cm) | No. of oocytes retrieved | No. of high-quality embryos | Mean No. of transferred embryos per cycle |
---|---|---|---|---|
30.02 ± 3.12 | 0.93 ± 0.42 | 13.52 ± 4.10 | 9.73 ± 4.82 | 2.16 ± 0.78 |
Sample number | The number of embryonic | Morphological classification |
---|---|---|
1 | 8 | A |
2 | 5 | C |
3 | 8 | B |
4 | 8 | A |
5 | 8 | A |
6 | 8 | A |
7 | 8 | B |
8 | 8 | A |
9 | 8 | B |
10 | 6 | B |
11 | 8 | B |
12 | 8 | B |
13 | 6 | B |
14 | 8 | B |
15 | 6 | C |
16 | 8 | B |
17 | 8 | B |
18 | 5 | B |
19 | 8 | C |
20 | 5 | C |
21 | 7 | B |
22 | 4 | B |
23 | 4 | B |
24 | 7 | B |
25 | 8 | A |
26 | 8 | B |
27 | 8 | B |
28 | 7 | B |
29 | 6 | B |
30 | 5 | B |
31 | 6 | B |
32 | 3 | B |
33 | 5 | B |
34 | 5 | B |
35 | 7 | B |
36 | 7 | B |
37 | 5 | B |
38 | 8 | B |
39 | 4 | C |
40 | 3 | C |
41 | 7 | B |
42 | 8 | B |
43 | 4 | C |
44 | 7 | B |
45 | 7 | B |
46 | 8 | B |
47 | 6 | B |
48 | 8 | C |
49 | 8 | A |
50 | 8 | A |
51 | — | fragment |
52 | 8 | B |
53 | 8 | B |
54 | 4 | B |
55 | 8 | B |
56 | 8 | B |
57 | 8 | C |
Component | Concentration (mM) |
---|---|
Sodium bicarbonate | 25.0 |
Potassium chloride | 2.5 |
Potassium phosphate | 0.35 |
Calcium chloride, anhydrous | 1.7 |
Sodium chloride | 101.5 |
Magnesium sulfate, anhydrous | 0.2 |
Sodium bicarbonate | 25.0 |
Sodium pyruvate | 0.2 |
Glucose | 0.5 |
Sodium citrate | 1.0 |
Sodium lactate (D/L) | 20 |
EDTA, disodium, dihydrate | 10 μm |
Alanine | 0.05 |
Arginine | 0.3 |
Alanyl-glutamine | 1.0 |
Asparagine | 0.05 |
Aspartic acid | 0.05 |
Cysteine | 0.05 |
Glutamic acid | 0.05 |
Glycine | 0.05 |
Histidine | 0.1 |
Isoleucine | 0.2 |
Leucine | 0.2 |
Lysine | 0.2 |
Methionine | 0.05 |
Phenylalanine | 0.1 |
Proline | 0.05 |
Serine | 0.05 |
Taurine | 0.05 |
Threonine | 0.2 |
Tryptophan | 0.02 |
Tyrosine | 0.1 |
Valine | 0.2 |
Phenol red | 4.8 mg/L |
Gentamicin | 10 ug/mL |
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Zhao, Q.; Yin, T.; Peng, J.; Zou, Y.; Yang, J.; Shen, A.; Hu, J. Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF). Int. J. Mol. Sci. 2013, 14, 6556-6570. https://doi.org/10.3390/ijms14046556
Zhao Q, Yin T, Peng J, Zou Y, Yang J, Shen A, Hu J. Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF). International Journal of Molecular Sciences. 2013; 14(4):6556-6570. https://doi.org/10.3390/ijms14046556
Chicago/Turabian StyleZhao, Qinghong, Tailang Yin, Jin Peng, Yujie Zou, Jing Yang, Aiguo Shen, and Jiming Hu. 2013. "Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF)" International Journal of Molecular Sciences 14, no. 4: 6556-6570. https://doi.org/10.3390/ijms14046556