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Review

tRNA Modification Enzymes GidA and MnmE: Potential Role in Virulence of Bacterial Pathogens

by
Daniel C. Shippy
and
Amin A. Fadl
*
Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(10), 18267-18280; https://doi.org/10.3390/ijms151018267
Submission received: 3 September 2014 / Revised: 2 October 2014 / Accepted: 8 October 2014 / Published: 10 October 2014
(This article belongs to the Special Issue Functions of Transfer RNAs)
Versions Notes

Abstract

:
Transfer RNA (tRNA) is an RNA molecule that carries amino acids to the ribosomes for protein synthesis. These tRNAs function at the peptidyl (P) and aminoacyl (A) binding sites of the ribosome during translation, with each codon being recognized by a specific tRNA. Due to this specificity, tRNA modification is essential for translational efficiency. Many enzymes have been implicated in the modification of bacterial tRNAs, and these enzymes may complex with one another or interact individually with the tRNA. Approximately, 100 tRNA modification enzymes have been identified with glucose-inhibited division (GidA) protein and MnmE being two of the enzymes studied. In Escherichia coli and Salmonella, GidA and MnmE bind together to form a functional complex responsible for the proper biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U34) of tRNAs. Studies have implicated this pathway in a major pathogenic regulatory mechanism as deletion of gidA and/or mnmE has attenuated several bacterial pathogens like Salmonella enterica serovar Typhimurium, Pseudomonas syringae, Aeromonas hydrophila, and many others. In this review, we summarize the potential role of the GidA/MnmE tRNA modification pathway in bacterial virulence, interactions with the host, and potential therapeutic strategies resulting from a greater understanding of this regulatory mechanism.

1. Introduction

RNA molecules consist of four basic nucleosides, adenosine (A), uridine (U), cytidine (C), and guanosine (G). Post-transcriptional RNA modifications are present in many types of RNAs including transfer RNA (tRNA), messenger RNA (mRNA), ribosomal RNA (rRNA), and others. Modifications consist of methylation of a ribose or base, thiolation, deamination, base isomerization, or complex hypermodifications [1]. These modifications are important for altering the chemical and physical properties of nucleotides resulting in increased efficiency of RNA functions.
Of all the RNAs, tRNAs display the greatest number and variety of modifications, with organisms from all domains of life allocating a large portion of their genome to encoding enzymes involved in the post-transcriptional modification of nucleosides in tRNA [2]. Post-transcriptional modification of the anticodon domain in tRNA is a major factor in controlling gene expression which allows bacteria to adapt and survive in many different types of environments [3]. Modifications of uridine at the wobble position of the anticodon (U34) are required for the recognition of rare codons. In the absence of modifications, a shift in the translational reading frame occurs resulting in the expression of alternate protein sequences [3].
Numerous enzymes have been identified in the modification pathways of bacterial tRNAs. For example, the IscS–MnmA pathway is responsible for thiolation at position 2 of U34 [4,5,6]. MnmA is a member of the ATP-pyrophosphatase family which recognizes nucleotides U34 and U35 of the anticodon region of bacterial tRNAs [6]. IscS is a highly conserved master enzyme that catalyzes the formation of alanine and sulfane sulfur from cysteine to provide sulfur for the IscS–MnmA pathway as well as three other modification pathways [7,8,9]. Other pathways include known tRNA modification enzymes like glucose-inhibited division (GidA) protein, MnmE. GidA has been classified into two groups based on the size of the protein with the larger protein, GidA (also known as MnmG), consisting of 611–679 amino acid residues, while the smaller protein, Gid (also known as TrmFO), contains 435–482 amino acid residues [10,11]. GidA was first described in Escherichia coli as a cell division protein because deletion of gidA resulted in a filamentous morphology when grown in a rich medium supplemented with glucose [12]. Further studies have suggested a role for GidA in the cell division and morphology of Salmonella enterica serovar Typhimurium (STM) and Aeromonas hydrophila [13,14]. Most importantly, studies in E. coli suggest GidA is a tRNA modification enzyme responsible for the proper biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the 5 position of the wobble uridine (U34) of tRNAs [15,16].
MnmE, also known as TrmE, is a multi-domain GTPase that is highly conserved amongst bacteria and eukarya [15]. GTPases are well known for regulating a number of cellular processes such as ribosome assembly, membrane trafficking, signal transduction, and protein biosynthesis [17]. The GTPase properties of MnmE, however, are very unique in that MnmE displays a hydrolysis rate at a low affinity for GTP and GDP in the presence of potassium ions suggesting that the MnmE GTPase cycle proceeds in an efficient manner without GTPase activating proteins and guanine nucleotide exchange factors [18,19,20]. Furthermore, similar to GidA, MnmE appears to be responsible for the proper biosynthesis of mnm5s2U at the 5 position of U34 of tRNAs [21,22].
In E. coli, the general hypothesis is that GidA and MnmE are part of the same tRNA modification pathway [21,22]. The study by Yim et al. reported that mutations in E. coli gidA impaired the biosynthesis of mnm5s2U. Interestingly, their study also showed identical levels of the same undermodified form of U34 are present in tRNA hydrolysates from gidA and mnmE mutants suggesting GidA and MnmE form a functional complex in which both proteins are interdependent [16]. Further studies done in E. coli have provided additional evidence suggesting the in vitro binding ability of GidA and MnmE and that together these two enzymes are responsible for the proper biosynthesis of mnm5s2U in bacterial tRNA [23,24,25]. Then, another enzyme, MnmC (formerly known as YfcK or TrmC), catalyzes a FAD-dependent deacetylation to 5-aminomethyl (nm5), and this group is subsequently methylated to produce methylaminomethyl (mnm5) [26,27,28,29]. Additionally, Shippy et al. showed that GidA and MnmE bind together to modify Salmonella tRNA [30].
One of the most interesting aspects of the GidA/MnmE tRNA modification pathway is its potential role as a pathogenic regulatory mechanism. GidA and MnmE are highly conserved proteins with homologues of GidA and MnmE in humans (MTO1 and GTPBP3, respectively) and yeast (MTO1 and MSS1, respectively) involved in modification of mitochondrial tRNAs [31,32,33,34]. Lack of modification has shown mitochondrial dysfunction in human cells, neurological and developmental dysfunctions in Caenorhabditis elegans, and attenuated numerous bacterial pathogens [10,14,18,30,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55]. Additionally, lack of modification has been associated with acute liver failure, lactic acidosis, hypertrophic cardiomyopathies, and mitochondrial encephalomyopathies in humans [56,57,58]. Within this review, we summarize the current knowledge of the GidA/MnmE tRNA modification pathway in the pathogenesis of bacterial pathogens.

2. GidA/MnmE-Associated Virulence of Gram-Negative Bacteria

GidA and MnmE have been implicated in the pathogenesis of members of the Enterobacteriaceae family including E. coli and Salmonella. MnmE was first described in E. coli as a molecular switch GTPase which assumes different conformations depending on whether it is bound to GTP or GDP [18]. Deletion of mnmE has been shown to be lethal in some E. coli strains, but shows no lethal consequences in other strains of E. coli [18]. MnmE has also been shown to activate the transcriptional regulator gadE, which is important in glutamate-dependent acid resistance in E. coli [40]. Furthermore, the study by Yu et al. shows that GidA is a potential regulator of cytotoxic necrotizing factor 1 (CNF1) which is an important toxin in meningitis-causing E. coli K1 [47]. In their study, gidA was identified in a transposon screening for genetic requirements of CNF1 production and secretion. A gidA deletion mutant had a drastic effect on CNF1 production, and they showed that GidA inhibited CNF1 at the translation level and is most likely part of a key mechanism in the modulation of CFN1 translation [47].
The most comprehensive investigation into the role of GidA and MnmE in pathogenesis was performed in Salmonella. In an initial study by Shippy et al., a gidA deletion mutant of the STM 14028 strain was significantly attenuated in vitro as indicated by reduced motility, replication in macrophages, invasion of intestinal epithelial cells, and cytotoxicity of macrophages [38]. Furthermore, a gidA deletion mutant was highly attenuated in mice as indicated by a significantly higher lethal dose 50 (LD50). In a mouse infection model of salmonellosis, a gidA mutant showed reduced colonization of the liver and spleen, reduced severity of histopathological lesions in the liver and spleen, and decreased levels of the proinflammatory cytokines when compared to mice infected with the wild-type STM 14028 strain [38]. Additional analysis into the virulence mechanism was performed using global transcriptional and proteomic profiling. Microarray analysis, deposited at the GEO database of the National Center for Biotechnology [59] under series number GSE30787, showed altered expression of numerous genes in the gidA mutant with genes of numerous functions being affected. Several virulence genes were shown to be down-regulated like the type 3 secretion system apparatus genes (invAEG, spaPQ, and prgHJ), flagellar genes (fliACDJKZ, flgLM, and flhAB), flagellar motor genes (motAB), and many others [38]. 2D gel electrophoresis and semi-quantitative Western blot analysis showed numerous proteins were altered in a gidA deletion mutant most notably a significant down-regulation of virulence proteins including PrgH, FliC, and MotB [38]. The down-regulation of these virulence genes and proteins provides a plausible correlation to the attenuated phenotypes observed in the gidA mutant, and identified virulence factors potentially regulated by GidA.
In a follow up study, Shippy et al. characterized a gidA, mnmE, and gidA mnmE deletion mutants of Salmonella to examine the relative contribution of GidA and MnmE in virulence. A gidA deletion mutant showed the same attenuated phenotypes as in their previous study [30,38]. The mnmE deletion mutant was significantly attenuated in both in vitro and in vivo models of Salmonella infection, but not to the extent of a gidA deletion mutant [30]. Meanwhile, a gidA mnmE deletion mutant was more attenuated than either single mutant, particularly in vivo as no mice died during the LD50 experiment that were infected with the gidA mnmE deletion mutant [30]. Additional data from this study showed a rather significant growth defect in the gidA and gidA mnmE deletion mutants with a modest growth rate decrease in the mnmE mutant when compared to the wild-type Salmonella strain [30]. An additional study showed that GidA protein expression was significantly increased under certain growth conditions (1% glucose, pH 5, 100 μM EDTA). Furthermore, this study showed that the increase in GidA expression correlated with increased in vitro Salmonella virulence as indicated by increased motility and cytotoxicity of macrophages [39].
Stress response systems play a crucial role in the virulence of pathogenic organisms [60,61]. The ability of bacteria to recognize and respond to adverse environments is required for their survival. These responses require a wide variety of global regulatory network systems [60,61]. In order to survive and establish infection, Salmonella and other bacteria must overcome a range of stress conditions including external environments, food matrices, and the in vivo host environment such as ability to survive inside macrophages [60,61]. A Salmonella gidA mutant showed a significant decrease in ability to survive and replicate inside macrophages and in a mouse model of infection [38]. Most importantly, expression of several stress related genes such as carbon starvation (cstA), putative universal stress (ynaF and yecG), heat shock (hslO), and cold-shock (cspH) genes were significantly altered in a gidA mutant, compared to the WT suggesting an important role for GidA in the response to these particular stressors [38]. Similarly, proteome analysis of a gidA mutant revealed down-regulation in expression of several proteins involved in survival of Salmonella to stressful conditions inside host macrophages such as the oxidoreductase YghA, and the thiol peroxidase Tpx [38].
To examine the potential GidA functional interactions with other proteins in Salmonella, Shippy et al. performed bioinformatic analysis using STRING 8.3 software [30]. Data from this analysis strongly suggest that GidA interacts with several other Salmonella proteins involved in stress response and replication (e.g., DnaA and DnaN) and RNA modification enzymes (MnmE, MnmA, and RsmG). On the other hand, MnmE interacts, in addition to GidA, with RNA modification enzymes (MnmA, RluD), stress and replication related proteins (DnaA, YhbZ, GyrB). These data strongly suggest a role for the GidA and MnmE in stress responses. Additionally, the study by Faron et al. suggests MnmE is part of a regulatory system which modulates the small alarmone ppGpp to coordinate Francisella tularensis gene expression in response to the numerous nutritional and cellular stresses present in different mammalian cells [43].
Several reports show the potential contribution of GidA and MnmE to the pathogenesis of other Gram-negative bacteria. A recent study by Zhang et al. suggests GidA and MnmE are positive regulatory elements that regulate the production of 2,4-diacetylphloroglucinol (2,4-DAPG) using a post-transcriptional mechanism in an important plant growth-promoting rhizobacteria, Pseudomonas fluorescens [41]. Both gidA and mnmE deletion mutants were unable to produce phloroglucinol (PG), but could convert PG to monoacetylphloroglucinol (MAPG) and MAPG to 2,4 DAPG suggesting a pathway independent of the Gac/Rsm two-component system [41]. These findings offer insight into the regulation of secondary metabolites, like 2,4-DAPG, which displays antimicrobial activity, as well as other functions important for plant growth and health [62,63,64].
In another plant pathogen, Pseudomonas syringae, GidA is hypothesized to be a global regulator of P. syringae, as deletion of gidA resulted in phenotypes associated with mutations of two-component regulators gacS and gacA [42,65,66]. In their study, a gidA deletion mutant lost the ability to produce the lipodepsipeptide antibiotics syringomycin and syringopeptin as well as the ability to swarm across the surface of low agar media [42]. Additionally, deletion of gidA attenuated the organism, but not to the extent seen in gacS, gacA, or salA deletion mutants [42,67]. In a different study in P. syringae, MnmE is hypothesized to be important for growth at low temperatures [48]. In their study, transposon screening identified a tmrE mutant with inhibited growth at 4 °C, but not at 22 and 28 °C when compared to the growth of the wild-type P. syringae Lz4W cells indicating the importance of MnmE for rapid growth at unfavorable conditions [48]. To our knowledge, the only other study involving either GidA or MnmE in Pseudomonas is in Pseudomonas aeruginosa, where GidA was found to regulate rhl quorum sensing using a post-transcriptional mechanism [68]. In their study, gidA was identified by transposon screening for mutants displaying deficient quorum sensing phenotypes. Further analysis of a gidA mutant showed a deficiency in rhl-dependent quorum sensing, growth, LasA activity, and pyocyanin production [68].
Other GidA studies in Gram-negative bacteria include Aeromonas hydrophila, an emerging human pathogen that causes gastroenteritis and septicemia, where GidA was found to regulate the cytotoxic entertoxin (Act) [14]. A gidA deletion mutant was avirulent in mice, with minimal to mild pathology, due to a significant reduction in hemolytic and cytotoxic activity [14]. In a follow up study, the same group found that act was under the control of both GidA and DNA adenine methyltransferase (Dam), and Dam regulates Act production via GidA [49]. In Myxococcus xanthus, GidA was found to be a FAD-binding protein involved in M. xanthus development where a gidA deletion mutant was unable to form fruiting bodies after several generations [10]. In Helicobacter pylori, Karita et al. found the gidA gene was in close proximity to the dapE gene (which encodes succinyl-diaminopimelate desuccinylase) and that the gidA gene was essential for cell viability [50]. These findings, however, do not provide evidence of GidA-associated viurlence, but does suggest GidA is essential for H. pylori viability [50]. To our knowledge, the only other study involving GidA or MnmE in Gram-negative bacteria is a recent study by Faron et al. where deletion of mnmE is associated with F. tularensis pathogenicity island gene expression [43]. In their study, a mnmE deletion mutant displayed reduced levels of the small alarmone ppGpp, as well as an altered growth rate in mammalian cells suggesting MnmE is an important part of a complex regulatory system responsible for coordinating virulence gene expression in different host cell environments [43].

3. GidA/MnmE-Associated Virulence of Gram-Positive Bacteria

The role of GidA and MnmE in the pathogenesis of Gram-positive bacteria has not been as extensively studied as in Gram-negative bacteria, but there is evidence of an association with pathogenesis, particularly in Streptococcus. In Streptococcus pyogenes, transposon mutagenesis of gidA resulted in reduced expression of SpeB, an important pyrogenic and cardiotoxic virulence factor in group A Streptococcus [37]. Further analysis showed speB transcription was delayed in a gidA mutant via the transcriptional activator RopB. Overall, a gidA deletion mutant displayed a nearly normal transcriptional profile of S. pyogenes virulence factors, but showed reduced expression of several virulence proteins including mitogenic factor (MF), streptolysin O (SLO), NAD-glycohydrolase (SPN), and surface M protein [37]. The reduced expression of these virulence proteins correlated to a gidA deletion mutant being highly attenuated in a murine subcutaneous ulcer model of soft tissue infection. Furthermore, their study showed that a mnmE deletion mutant displayed the same phenotypes displayed by a gidA deletion mutant [37].
GidA has also been associated with virulence of other Streptococcus bacteria as well. In Streptococcus mutans, random-insertion mutagenesis identified a gidA mutant with reduced ability to withstand stressful conditions, such as low pH, high osmotic pressure, high temperature, and bacitracin exposure [44]. Additionally, loss of GidA and/or MnmE resulted in a 50% decrease in glucose-dependent biofilm formation, a key virulence property of S. mutans. Interestingly, loss of GidA did not result in reduced expression of the most common secreted and cell surface virulence protein of S. mutans [44]. Another study in Streptococcus suis serotype 2 (SS2), the most prevalent and virulent serotype, showed gidA was up-regulated in vivo during experimental infection in pigs suggesting GidA is an important virulence factor in SS2 infection [45].
To date, the only other study of Gram-positive bacteria involving GidA was in Lactococcus garvieae, an emerging aquaculture pathogen. In this study, signature-tagged mutagenesis identified a deletion mutant homologous to gidA that displayed a reduced ability to grow in rainbow trout [46]. This preliminary genome-wide scan for virulence factors of L. garvieae could provide targets for future therapeutic strategies due to its association with mastitis in domestic animals and endocarditis and septicemia in humans [46].

4. Potential Mechanism of Virulence

In E. coli and Salmonella, it has been shown that GidA and MnmE bind together to modify tRNA using a post-transcriptional mechanism in which both proteins are interdependent [16,21,22,30]. GidA and MnmE were first identified as potential tRNA modification enzymes during a screen for novel E. coli mutants defective in mnm5s2U biosynthesis [21]. Crystal structures of MnmE show a dimeric protein with each monomer consisting of three domains which are the N-terminal, central helical, and GTP-binding domains [69,70]. The crystal structure of GidA reveals a FAD-binding site and dimer interface [24,25,71]. Each GidA homodimer subunit consists of three domains which are the first α/β domain (also called the FAD-binding domain), a second α/β domain, and a α-helical C-terminal domain [24,25,71]. The mechanism by which GidA and MnmE form a heterotetrameric complex is not fully understood, but it has been shown that the α-helical C-terminal domain of GidA along with the two-fold symmetry N-terminal domain of MnmE are required for GidA/MnmE interaction [24,25,71]. Once the GidA/MnmE interaction occurs, the complex catalyzes two different GTP-and FAD-dependent reactions which results in 5-aminomethyluridine (nm5U) and 5-carboxymethylaminomethyluridine (cmnm5U) by using ammonium and glycine, respectively, as substrates [23]. GTP hydrolysis by the MnmE G-domain leads to structural rearrangements of the GidA/MnmE complex which has been shown to be essential for tRNA modification [15,72]. More work is needed, however, to determine the mechanism of tRNA recognition and substrate selection by the GidA/MnmE complex.
The GidA/MnmE pathway has been implicated as a major pathogenic regulatory mechanism in bacteria as evidenced by the numerous pathogens that have been attenuated following deletion of gidA and/or mnmE. The mechanism by which this pathway regulates bacterial virulence is unclear. It has been shown that deletion of gidA and/or mnmE increases the occurrence of a two-base frameshift during the translation of mRNA [22]. This hypothesis is plausible as it is well known that modifications at the wobble position of the anticodon are needed to accurately and efficiently synthesize proteins [73,74]. Furthermore, alterations in the modification levels of the wobble position can affect the synthesis of specific proteins [73,75,76,77]. This mechanism of translational fidelity suggests a role of global regulation by GidA and MnmE as evidenced by their mutations affecting numerous phenotypic traits.
Despite the conservation of GidA and MnmE among different bacteria, the phenotypes of different gidA and/or mnmE mutants do not suggest a common mechanism for the functions of these enzymes in bacterial pathogens. This could be due to the prevention of frameshifting of genes encoding virulence proteins in the different organisms since base modification contributes to the efficiency and accuracy of translation [3,22,78]. Also, studies of GidA have been complicated because homologues of GidA exist in two sizes, and are transcribed from genes that are not genetically linked [10]. For example, the study by Cho et al. shows that deletion of gidA results in a nearly normal transcriptional profile with an alteration of several virulence proteins. Additionally, their study showed deletion of mnmE resulted in nearly the same phenotypes as deletion of gidA [37]. In contrast, studies in Salmonella show that deletion of gidA results in a down-regulation of both virulence genes and proteins, and although deletion of mnmE results in the same attenuated phenotypes as a gidA deletion mutant, a gidA deletion mutant appears to be more attenuated than a mnmE deletion mutant [30,38]. One plausible explanation for this could be their location in the chromosome. The gidA gene is located in close proximity to the oriC, and deletion of gidA results in a filamentous morphology [13,14,38,79,80,81,82]. In contrast, mnmE is not located near the oriC, and deletion of mnmE does not result in a filamentous morphology indicating MnmE has no role in Salmonella cell division. These data shows that not only can these mutants have differing severity of phenotypes, but can display entirely different phenotypes all together. Another plausible explanation for the differing severity of phenotypes observed is that these enzymes are involved in other pathways. In one of our studies, analysis with STRING 8.3 software showed a high level of predicted functional association between GidA and MnmE, but also showed numerous other proteins GidA and MnmE could potentially bind with, indicating other pathways these enzymes could be associated with [30]. This is not surprising since wobble modifications are very complex and require the participation of numerous enzymes to carry out these processes [73]. Approximately 85 modifications have been identified in tRNA molecules, involving numerous different enzymes, with most modifications being found at positions 34 and 37 of the anticodon stem loop [73]. Not all of these enzymes and pathways are associated with bacterial virulence; therefore, much work is still needed to elucidate the exact mechanism of the GidA/MnmE modification pathway in relationship with bacterial virulence.

5. Potential Therapeutic Benefits

A major benefit of these attenuated bacterial strains is their potential use in live-attenuated vaccines. In Gram-positive bacteria, the study by Cho et al. shows tRNA modification by GidA and MnmE is essential for S. pyogenes virulence, and suggests deletion of genes encoding tRNA modification enzymes as a new strategy to make avirulent strains for use in live-attenuated vaccines [37]. Their study also showed that deletion of mnmE resulted in the same phenotype as a gidA deletion mutant, and these mutants were highly attenuated in the murine ulcer model of soft tissue infection [37]. Furthermore, these mutants displayed a cytokine profile in cultured macrophages identical to that of the wild-type S. pyogenes strain, with the exception of reduced levels of IL-23 and TNFα [37].
The most extensive investigation into the use of these mutants in a live-attenuated vaccine has been performed by Shippy et al. in the enteric pathogen Salmonella [30,38,83]. In our preliminary studies, gidA, mnmE, and gidA mnmE deletion mutants were highly attenuated in mice, and immunization with these mutants protected mice from challenge with a highly lethal dose of the wild-type Salmonella strain [30,38]. In another study by Shippy et al., a more extensive investigation into the use of a gidA mutant in a live-attenuated vaccine was performed to characterize the protective immune response conferred by immunization [83]. This study showed immunization with a gidA mutant elicited a mixed Th1/Th2 response as indicated by increased levels of IgG1 and IgG2a in the sera of immunized mice. Lymphocytes from immunized mice showed a marked proliferative response to treatment with Salmonella lysate, and culture supernatants showed an increase in IL-2, IFN-γ, and IL-10 levels [83]. Furthermore, passive-immunization studies showed that lymphocytes and sera from immunized mice could partially protect mice from a highly lethal dose of the wild-type Salmonella strain [83]. Overall, these studies suggest these mutants could be promising candidates for use in a live-attenuated vaccine, or as a vaccine vector to deliver antigens from other pathogens to induce immunity in the vaccinated host.

6. Conclusions

Overall, the GidA/MnmE tRNA modification pathway appears to be part of a major virulence mechanism in bacteria. Further study is needed to identify and characterize other enzymes potentially involved in this pathway as well as other modification pathways GidA and MnmE are associated with. Additionally, more studies are needed to determine if GidA and MnmE regulate the specific genes and proteins identified for the pathogenic processes these enzymes are involved in, or if alterations in tRNA modifications result in pleiotropic phenotypes. In turn, these observations could help establish a more definitive link between tRNA modification and bacterial virulence. Most importantly, investigation into a gidA mnmE double deletion mutant for use in a live-attenuated vaccine, or as a vaccine vector, could lead to a promising therapeutic strategy to control or prevent disease.

Acknowledgments

The research in our laboratory was supported by grants from the USDA Hatch Fund.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. McCloskey, J.A.; Crain, P.F. The RNA modification database—1998. Nucleic Acids Res. 1998, 26, 196–197. [Google Scholar]
  2. Agris, P.F.; Vendeix, F.A.; Graham, W.D. tRNA’s wobble decoding of the genome: 40 years of modification. J. Mol. Biol. 2007, 366, 1–13. [Google Scholar]
  3. Gustilo, E.M.; Vendeix, F.A.; Agris, P.F. tRNA’s modifications bring order to gene expression. Curr. Opin. Microbiol. 2008, 11, 134–140. [Google Scholar]
  4. Kambampati, R.; Lauhon, C.T. MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli. Biochemistry 2003, 42, 1109–1117. [Google Scholar]
  5. Ikeuchi, Y.; Shigi, N.; Kato, J.; Nishimura, A.; Suzuki, T. Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions. Mol. Cell. 2006, 21, 97–108. [Google Scholar]
  6. Numata, T.; Ikeuchi, Y.; Fukai, S.; Suzuki, T.; Nureki, O. Snapshots of tRNA sulphuration via an adenylated intermediate. Nature 2006, 442, 419–424. [Google Scholar]
  7. Lauhon, C.T. Requirement for IscS in biosynthesis of all thionucleosides in Escherichia coli. J. Bacteriol. 2002, 184, 6820–6829. [Google Scholar]
  8. Nilsson, K.; Lundgren, H.K.; Hagervall, T.G.; Björk, G.R. The cysteine desulfurase IscS is required for synthesis of all five thiolated nucleosides present in tRNA from Salmonella enterica serovar Typhimurium. J. Bacteriol. 2002, 184, 6830–6835. [Google Scholar]
  9. Lundgren, H.K.; Björk, G.R. Structural alterations of the cysteine desulfurase IscS of Salmonella enterica serovar Typhimurium reveal substrate specificity of IscS in tRNA thiolation. J. Bacteriol. 2006, 188, 3052–3062. [Google Scholar]
  10. White, D.J.; Merod, R.; Thomasson, B.; Hartzell, P.L. GidA is an FAD-binding protein involved in development of Myxococcus xanthus. Mol. Microbiol. 2001, 42, 503–517. [Google Scholar]
  11. Urbonavicius, J.; Skouloubris, S.; Myllykallio, H.; Grosjean, H. Identification of a novel gene encoding a flavin-dependent tRNA:m5U methyltransferase in bacteria—Evolutionary implications. Nucleic Acids Res. 2005, 33, 3955–3964. [Google Scholar]
  12. Von Meyenburg, K.; Jorgensen, B.B.; Nielsen, J.; Hansen, F.G. Promoters of the atp operon coding for the membrane-bound ATP synthase of Escherichia coli mapped by Tn10 insertion mutations. Mol. Gen. Genet. 1982, 188, 240–248. [Google Scholar]
  13. Shippy, D.C.; Heintz, J.A.; Albrecht, R.M.; Eakley, N.M.; Chopra, A.K.; Fadl, A.A. Deletion of glucose-inhibited division (gidA) gene alters the morphological and replication characteristics of Salmonella enterica serovar Typhimurium. Arch. Microbiol. 2012, 194, 405–412. [Google Scholar]
  14. Sha, J.; Kozlova, E.V.; Fadl, A.A.; Olano, J.P.; Houston, C.W.; Peterson, J.W.; Chopra, A.K. Molecular characterization of a glucose-inhibited division gene, gidA, that regulates cytotoxic enterotoxin of Aeromonas hydrophila. Infect. Immun. 2004, 72, 1084–1095. [Google Scholar]
  15. Armengod, M.E.; Moukadiri, I.; Prado, S.; Ruiz-Partida, R.; Benitez-Paez, A.; Villarroya, M.; Lomas, R.; Garzon, M.J.; Martinez-Zamora, A.; Meseguer, S.; et al. Enzymology of tRNA modification in the bacterial MnmEG pathway. Biochimie 2012, 94, 1510–1520. [Google Scholar]
  16. Yim, L.; Moukadiri, I.; Björk, G.R.; Armengod, M.E. Further insights into the tRNA modification process controlled by proteins MnmE and GidA of Escherichia coli. Nucleic Acids Res. 2006, 34, 5892–5905. [Google Scholar]
  17. Wittinghofer, A.; Vetter, I.R. Structure–function relationships of the G domain, a canonical switch motif. Annu. Rev. Biochem. 2011, 80, 943–971. [Google Scholar]
  18. Cabedo, H.; Macian, F.; Villarroya, M.; Escudero, J.C.; Martinez-Vicente, M.; Knecht, E.; Armengod, M.E. The Escherichia coli trmE (mnmE) gene, involved in tRNA modification, codes for an evolutionarily conserved GTPase with unusual biochemical properties. EMBO J. 1999, 18, 7063–7076. [Google Scholar]
  19. Yamanaka, K.; Hwang, J.; Inouye, M. Characterization of GTPase activity of TrmE, a member of a novel GTPase superfamily, from Thermotoga maritima. J. Bacteriol. 2000, 182, 7078–7082. [Google Scholar]
  20. Scrima, A.; Wittinghofer, A. Dimerisation-dependent GTPase reaction of MnmE: How potassium acts as GTPase-activating element. EMBO J. 2006, 25, 2940–2951. [Google Scholar]
  21. Elseviers, D.; Petrullo, L.A.; Gallagher, P.J. Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2-thiouridine. Nucleic Acids Res. 1984, 12, 3521–3534. [Google Scholar]
  22. Bregeon, D.; Colot, V.; Radman, M.; Taddei, F. Translational misreading: A tRNA modification counteracts a +2 ribosomal frameshift. Genes Dev. 2001, 15, 2295–2306. [Google Scholar]
  23. Moukadiri, I.; Prado, S.; Piera, J.; Velazquez-Campoy, A.; Björk, G.R.; Armengod, M.E. Evolutionarily conserved proteins MnmE and GidA catalyze the formation of two methyluridine derivatives at tRNA wobble positions. Nucleic Acids Res. 2009, 37, 7177–7193. [Google Scholar]
  24. Shi, R.; Villarroya, M.; Ruiz-Partida, R.; Li, Y.; Proteau, A.; Prado, S.; Moukadiri, I.; Benitez-Paez, A.; Lomas, R.; Wagner, J.; et al. Structure–function analysis of Escherichia coli MnmG (GidA), a highly conserved tRNA-modifying enzyme. J. Bacteriol. 2009, 191, 7614–7619. [Google Scholar]
  25. Osawa, T.; Ito, K.; Inanaga, H.; Nureki, O.; Tomita, K.; Numata, T. Conserved cysteine residues of GidA are essential for biogenesis of 5-carboxymethylaminomethyluridine at tRNA anticodon. Structure 2009, 17, 713–724. [Google Scholar]
  26. Hagervall, T.G.; Edmonds, C.G.; McCloskey, J.A.; Björk, G.R. Transfer RNA(5-methylaminomethyl-2-thiouridine)-methyltransferase from Escherichia coli K-12 has two enzymatic activities. J. Biol. Chem. 1987, 262, 8488–8495. [Google Scholar]
  27. Bujnicki, J.M.; Oudjama, Y.; Roovers, M.; Owczarek, S.; Caillet, J.; Droogmans, L. Identification of a bifunctional enzyme MnmC involved in the biosynthesis of a hypermodified uridine in the wobble position of tRNA. RNA 2004, 10, 1236–1242. [Google Scholar]
  28. Roovers, M.; Oudjama, Y.; Kaminska, K.H.; Purta, E.; Caillet, J.; Droogmans, L.; Bujnicki, J.M. Sequence–structure–function analysis of the bifunctional enzyme MnmC that catalyses the last two steps in the biosynthesis of hypermodified nucleoside mnm5s2u in tRNA. Proteins 2008, 71, 2076–2085. [Google Scholar]
  29. Pearson, D.; Carell, T. Assay of both activities of the bifunctional tRNA-modifying enzyme MnmC reveals a kinetic basis for selective full modification of cmnm5s2u to mnm5s2u. Nucleic Acids Res. 2011, 39, 4818–4826. [Google Scholar]
  30. Shippy, D.C.; Eakley, N.M.; Lauhon, C.T.; Bochsler, P.N.; Fadl, A.A. Virulence characteristics of Salmonella following deletion of genes encoding the tRNA modification enzymes GidA and MnmE. Microb. Pathog. 2013, 57, 1–9. [Google Scholar]
  31. Decoster, E.; Vassal, A.; Faye, G. MSS1, a nuclear-encoded mitochondrial GTPase involved in the expression of COX1 subunit of cytochrome c oxidase. J. Mol. Biol. 1993, 232, 79–88. [Google Scholar]
  32. Colby, G.; Wu, M.; Tzagoloff, A. MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae. J. Biol. Chem. 1998, 273, 27945–27952. [Google Scholar]
  33. Li, X.; Guan, M.X. A human mitochondrial GTP binding protein related to tRNA modification may modulate phenotypic expression of the deafness-associated mitochondrial 12s rRNA mutation. Mol. Cell. Biol. 2002, 22, 7701–7711. [Google Scholar]
  34. Li, X.; Li, R.; Lin, X.; Guan, M.X. Isolation and characterization of the putative nuclear modifier gene MTO1 involved in the pathogenesis of deafness-associated mitochondrial 12S rRNA A1555G mutation. J. Biol. Chem. 2002, 277, 27256–27264. [Google Scholar]
  35. Chen, C.; Tuck, S.; Bystrom, A.S. Defects in tRNA modification associated with neurological and developmental dysfunctions in Caenorhabditis elegans elongator mutants. PLoS Genet. 2009, 5, e1000561. [Google Scholar]
  36. Villarroya, M.; Prado, S.; Esteve, J.M.; Soriano, M.A.; Aguado, C.; Perez-Martinez, D.; Martinez-Ferrandis, J.I.; Yim, L.; Victor, V.M.; Cebolla, E.; et al. Characterization of human GTPBP3, a GTP-binding protein involved in mitochondrial tRNA modification. Mol. Cell. Biol. 2008, 28, 7514–7531. [Google Scholar]
  37. Cho, K.H.; Caparon, M.G. tRNA modification by GidA/MnmE is necessary for Streptococcus pyogenes virulence: A new strategy to make live attenuated strains. Infect. Immun. 2008, 76, 3176–3186. [Google Scholar]
  38. Shippy, D.C.; Eakley, N.M.; Bochsler, P.N.; Chopra, A.K.; Fadl, A.A. Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of glucose-inhibited division (gidA) gene. Microb. Pathog. 2011, 50, 303–313. [Google Scholar]
  39. Rehl, J.M.; Shippy, D.C.; Eakley, N.M.; Brevik, M.D.; Sand, J.M.; Cook, M.E.; Fadl, A.A. GidA expression in Salmonella is modulated under certain environmental conditions. Curr. Microbiol. 2013, 67, 279–285. [Google Scholar]
  40. Gong, S.; Ma, Z.; Foster, J.W. The era-like GTPase TrmE conditionally activates gadE and glutamate-dependent acid resistance in Escherichia coli. Mol. Microbiol. 2004, 54, 948–961. [Google Scholar]
  41. Zhang, W.; Zhao, Z.; Zhang, B.; Wu, X.G.; Ren, Z.G.; Zhang, L.Q. Posttranscriptional regulation of 2,4-diacetylphloroglucinol production by GidA and TrmE in Pseudomonas fluorescens 2p24. Appl. Environ. Microbiol. 2014, 80, 3972–3981. [Google Scholar]
  42. Kinscherf, T.G.; Willis, D.K. Global regulation by GidA in Pseudomonas syringae. J. Bacteriol. 2002, 184, 2281–2286. [Google Scholar]
  43. Faron, M.; Fletcher, J.R.; Rasmussen, J.A.; Long, M.E.; Allen, L.A.; Jones, B.D. The Francisella tularensis migR, trmE, and cphA genes contribute to F. tularensis pathogenicity island gene regulation and intracellular growth by modulation of the stress alarmone ppGpp. Infect. Immun. 2013, 81, 2800–2811. [Google Scholar]
  44. Li, D.; Shibata, Y.; Takeshita, T.; Yamashita, Y. A novel gene involved in the survival of Streptococcus mutans under stress conditions. Appl. Environ. Microbiol. 2014, 80, 97–103. [Google Scholar]
  45. Li, W.; Liu, L.; Qiu, D.; Chen, H.; Zhou, R. Identification of Streptococcus suis serotype 2 genes preferentially expressed in the natural host. Int. J. Med. Microbiol. 2010, 300, 482–488. [Google Scholar]
  46. Menendez, A.; Fernandez, L.; Reimundo, P.; Guijarro, J.A. Genes required for Lactococcus garvieae survival in a fish host. Microbiology 2007, 153, 3286–3294. [Google Scholar]
  47. Yu, H.; Kim, K.S. mRNA context dependent regulation of cytotoxic necrotizing factor 1 translation by GidA, a tRNA modification enzyme in Escherichia coli. Gene 2012, 491, 116–122. [Google Scholar]
  48. Singh, A.K.; Pindi, P.K.; Dube, S.; Sundareswaran, V.R.; Shivaji, S. Importance of TrmE for growth of the psychrophile Pseudomonas syringae at low temperatures. Appl. Environ. Microbiol. 2009, 75, 4419–4426. [Google Scholar]
  49. Erova, T.E.; Kosykh, V.G.; Sha, J.; Chopra, A.K. DNA adenine methyltransferase (DAM) controls the expression of the cytotoxic enterotoxin (act) gene of Aeromonas hydrophila via tRNA modifying enzyme-glucose-inhibited division protein (GidA). Gene 2012, 498, 280–287. [Google Scholar]
  50. Karita, M.; Etterbeek, M.L.; Forsyth, M.H.; Tummuru, M.K.; Blaser, M.J. Characterization of Helicobacter pylori dapE and construction of a conditionally lethal dapE mutant. Infect. Immun. 1997, 65, 4158–4164. [Google Scholar]
  51. Durand, J.M.; Bjork, G.R.; Kuwae, A.; Yoshikawa, M.; Sasakawa, C. The modified nucleoside 2-methylthio-n6-isopentenyladenosine in tRNA of Shigella flexneri is required for expression of virulence genes. J. Bacteriol. 1997, 179, 5777–5782. [Google Scholar]
  52. Durand, J.M.; Okada, N.; Tobe, T.; Watarai, M.; Fukuda, I.; Suzuki, T.; Nakata, N.; Komatsu, K.; Yoshikawa, M.; Sasakawa, C. vacC, a virulence-associated chromosomal locus of Shigella flexneri, is homologous to tgt, a gene encoding tRNA-guanine transglycosylase (Tgt) of Escherichia coli K-12. J. Bacteriol. 1994, 176, 4627–4634. [Google Scholar]
  53. Patil, A.; Chan, C.T.; Dyavaiah, M.; Rooney, J.P.; Dedon, P.C.; Begley, T.J. Translational infidelity-induced protein stress results from a deficiency in Trm9-catalyzed tRNA modifications. RNA Biol. 2012, 9, 990–1001. [Google Scholar]
  54. Lamichhane, T.N.; Blewett, N.H.; Crawford, A.K.; Cherkasova, V.A.; Iben, J.R.; Begley, T.J.; Farabaugh, P.J.; Maraia, R.J. Lack of tRNA modification isopentenyl-A37 alters mRNA decoding and causes metabolic deficiencies in fission yeast. Mol. Cell. Biol. 2013, 33, 2918–2929. [Google Scholar]
  55. Dedon, P.C.; Begley, T.J. A system of RNA modifications and biased codon use controls cellular stress response at the level of translation. Chem. Res. Toxicol. 2014, 27, 330–337. [Google Scholar]
  56. Kirino, Y.; Suzuki, T. Human mitochondrial diseases associated with tRNA wobble modification deficiency. RNA Biol. 2005, 2, 41–44. [Google Scholar]
  57. Zeharia, A.; Shaag, A.; Pappo, O.; Mager-Heckel, A.M.; Saada, A.; Beinat, M.; Karicheva, O.; Mandel, H.; Ofek, N.; Segel, R.; et al. Acute infantile liver failure due to mutations in the trmU gene. Am. J. Hum. Genet. 2009, 85, 401–407. [Google Scholar]
  58. Ghezzi, D.; Baruffini, E.; Haack, T.B.; Invernizzi, F.; Melchionda, L.; Dallabona, C.; Strom, T.M.; Parini, R.; Burlina, A.B.; Meitinger, T.; et al. Mutations of the mitochondrial-tRNA modifier MTO1 cause hypertrophic cardiomyopathy and lactic acidosis. Am. J. Hum. Genet. 2012, 90, 1079–1087. [Google Scholar]
  59. Gene Expression Omnibus. Available online: http://www.ncbi.nlm.nih.gov/geo (accessed on 9 October 2014).
  60. Chaturongakul, S.; Raengpradub, S.; Wiedmann, M.; Boor, K.J. Modulation of stress and virulence in Listeria monocytogenes. Trends Microbiol. 2008, 16, 388–396. [Google Scholar]
  61. Runkel, S.; Wells, H.C.; Rowley, G. Living with stress: A lesson from the enteric pathogen Salmonella enterica. Adv. Appl. Microbiol. 2013, 83, 87–144. [Google Scholar]
  62. Cronin, D.; Moenne-Loccoz, Y.; Fenton, A.; Dunne, C.; Dowling, D.N.; O’Gara, F. Role of 2,4-diacetylphloroglucinol in the interactions of the biocontrol pseudomonad strain F113 with the potato cyst nematode Globodera rostochiensis. Appl. Environ. Microbiol. 1997, 63, 1357–1361. [Google Scholar]
  63. Phillips, D.A.; Fox, T.C.; King, M.D.; Bhuvaneswari, T.V.; Teuber, L.R. Microbial products trigger amino acid exudation from plant roots. Plant Physiol. 2004, 136, 2887–2894. [Google Scholar]
  64. Brazelton, J.N.; Pfeufer, E.E.; Sweat, T.A.; Gardener, B.B.; Coenen, C. 2,4-Diacetylphloroglucinol alters plant root development. Mol. Plant Microb. Interact. 2008, 21, 1349–1358. [Google Scholar]
  65. Hrabak, E.M.; Willis, D.K. The lemA gene required for pathogenicity of Pseudomonas syringae pv. Syringae on bean is a member of a family of two-component regulators. J. Bacteriol. 1992, 174, 3011–3020. [Google Scholar]
  66. Rich, J.J.; Kinscherf, T.G.; Kitten, T.; Willis, D.K. Genetic evidence that the gacA gene encodes the cognate response regulator for the lemA sensor in Pseudomonas syringae. J. Bacteriol 1994, 176, 7468–7475. [Google Scholar]
  67. Rich, J.J.; Willis, D.K. Multiple loci of Pseudomonas syringae pv. Syringae are involved in pathogenicity on bean: Restoration of one lesion-deficient mutant requires two tRNA genes. J. Bacteriol. 1997, 179, 2247–2258. [Google Scholar]
  68. Gupta, R.; Gobble, T.R.; Schuster, M. GidA posttranscriptionally regulates rhl quorum sensing in Pseudomonas aeruginosa. J. Bacteriol. 2009, 191, 5785–5792. [Google Scholar]
  69. Scrima, A.; Vetter, I.R.; Armengod, M.E.; Wittinghofer, A. The structure of the TrmE GTP-binding protein and its implications for tRNA modification. EMBO J. 2005, 24, 23–33. [Google Scholar]
  70. Meyer, S.; Böhme, S.; Kruger, A.; Steinhoff, H.J.; Klare, J.P.; Wittinghofer, A. Kissing G domains of MnmE monitored by X-ray crystallography and pulse electron paramagnetic resonance spectroscopy. PLoS Biol. 2009, 7, e1000212. [Google Scholar]
  71. Meyer, S.; Scrima, A.; Versees, W.; Wittinghofer, A. Crystal structures of the conserved tRNA-modifying enzyme GidA: Implications for its interaction with MnmE and substrate. J. Mol. Biol. 2008, 380, 532–547. [Google Scholar]
  72. Prado, S.; Villarroya, M.; Medina, M.; Armengod, M.E. The tRNA-modifying function of MnmE is controlled by post-hydrolysis steps of its GTPase cycle. Nucleic Acids Res. 2013, 41, 6190–6208. [Google Scholar]
  73. El Yacoubi, B.; Bailly, M.; de Crecy-Lagard, V. Biosynthesis and function of posttranscriptional modifications of transfer RNAs. Annu. Rev. Genet. 2012, 46, 69–95. [Google Scholar]
  74. Agris, P.F. Bringing order to translation: The contributions of transfer RNA anticodon-domain modifications. EMBO Rep. 2008, 9, 629–635. [Google Scholar]
  75. Phizicky, E.M.; Hopper, A.K. tRNA biology charges to the front. Genes Dev. 2010, 24, 1832–1860. [Google Scholar]
  76. Chan, C.T.; Pang, Y.L.; Deng, W.; Babu, I.R.; Dyavaiah, M.; Begley, T.J.; Dedon, P.C. Reprogramming of tRNA modifications controls the oxidative stress response by codon-biased translation of proteins. Nat. Commun. 2012, 3, 937. [Google Scholar]
  77. Chen, C.; Huang, B.; Eliasson, M.; Ryden, P.; Bystrom, A.S. Elongator complex influences telomeric gene silencing and DNA damage response by its role in wobble uridine tRNA modification. PLoS Genet. 2011, 7, e1002258. [Google Scholar]
  78. Urbonavicius, J.; Qian, Q.; Durand, J.M.; Hagervall, T.G.; Björk, G.R. Improvement of reading frame maintenance is a common function for several tRNA modifications. EMBO J. 2001, 20, 4863–4873. [Google Scholar]
  79. Gielow, A.; Kucherer, C.; Kolling, R.; Messer, W. Transcription in the region of the replication origin, oriC, of Escherichia coli: Termination of asnC transcripts. Mol. Gen. Genet. 1988, 214, 474–481. [Google Scholar]
  80. Kolling, R.; Gielow, A.; Seufert, W.; Kucherer, C.; Messer, W. Asnc, a multifunctional regulator of genes located around the replication origin of Escherichia coli, oriC. Mol. Gen. Genet. 1988, 212, 99–104. [Google Scholar]
  81. Ogawa, T.; Okazaki, T. Concurrent transcription from the gid and mioC promoters activates replication of an Escherichia coli minichromosome. Mol. Gen. Genet. 1991, 230, 193–200. [Google Scholar]
  82. Zhou, P.; Bogan, J.A.; Welch, K.; Pickett, S.R.; Wang, H.J.; Zaritsky, A.; Helmstetter, C.E. Gene transcription and chromosome replication in Escherichia coli. J. Bacteriol. 1997, 179, 163–169. [Google Scholar]
  83. Shippy, D.C.; Fadl, A.A. Immunological characterization of a gidA mutant strain of Salmonella for potential use in a live-attenuated vaccine. BMC Microbiol. 2012, 12, 286. [Google Scholar]

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Shippy, D.C.; Fadl, A.A. tRNA Modification Enzymes GidA and MnmE: Potential Role in Virulence of Bacterial Pathogens. Int. J. Mol. Sci. 2014, 15, 18267-18280. https://doi.org/10.3390/ijms151018267

AMA Style

Shippy DC, Fadl AA. tRNA Modification Enzymes GidA and MnmE: Potential Role in Virulence of Bacterial Pathogens. International Journal of Molecular Sciences. 2014; 15(10):18267-18280. https://doi.org/10.3390/ijms151018267

Chicago/Turabian Style

Shippy, Daniel C., and Amin A. Fadl. 2014. "tRNA Modification Enzymes GidA and MnmE: Potential Role in Virulence of Bacterial Pathogens" International Journal of Molecular Sciences 15, no. 10: 18267-18280. https://doi.org/10.3390/ijms151018267

APA Style

Shippy, D. C., & Fadl, A. A. (2014). tRNA Modification Enzymes GidA and MnmE: Potential Role in Virulence of Bacterial Pathogens. International Journal of Molecular Sciences, 15(10), 18267-18280. https://doi.org/10.3390/ijms151018267

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