Evaluating the Role of PTH in Promotion of Chondrosarcoma Cell Proliferation and Invasion by Inhibiting Primary Cilia Expression
Abstract
:1. Introduction
2. Results and Discussion
2.1. Human Chondrosarcoma Tissue Expressed an Elevated Level of PTHrP
2.2. PTH Promotes Chondrosarcoma Cell Proliferation by Suppressing Primary Cilia Assembly
2.3. PTH Enhances Chondrosarcoma Cells Invasion Capacity by Interacting with Primary Cilia
2.4. PTH/PTHrP Can Regulate Primary Cilia Expression
2.5. PTH/PTHrP Can Affect Hh Signaling Pathway Downstream Gene Expression
3. Experimental Section
3.1. Cells and Reagents
3.2. Cell Proliferation Test
3.3. Transwell Invasion Assay
3.4. Immunohistochemical Studies
3.5. Immunofluorescence Assay
3.6. Western Blot Analysis
3.7. Quantitative Real-Time PCR (qRT-PCR)
3.8. Small-Interfering RNA Transfection
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Xiang, W.; Jiang, T.; Guo, F.; Xu, T.; Gong, C.; Cheng, P.; Zhao, L.; Cheng, W.; Xu, K. Evaluating the Role of PTH in Promotion of Chondrosarcoma Cell Proliferation and Invasion by Inhibiting Primary Cilia Expression. Int. J. Mol. Sci. 2014, 15, 19816-19831. https://doi.org/10.3390/ijms151119816
Xiang W, Jiang T, Guo F, Xu T, Gong C, Cheng P, Zhao L, Cheng W, Xu K. Evaluating the Role of PTH in Promotion of Chondrosarcoma Cell Proliferation and Invasion by Inhibiting Primary Cilia Expression. International Journal of Molecular Sciences. 2014; 15(11):19816-19831. https://doi.org/10.3390/ijms151119816
Chicago/Turabian StyleXiang, Wei, Ting Jiang, Fengjing Guo, Tao Xu, Chen Gong, Peng Cheng, Libo Zhao, Weiting Cheng, and Kai Xu. 2014. "Evaluating the Role of PTH in Promotion of Chondrosarcoma Cell Proliferation and Invasion by Inhibiting Primary Cilia Expression" International Journal of Molecular Sciences 15, no. 11: 19816-19831. https://doi.org/10.3390/ijms151119816
APA StyleXiang, W., Jiang, T., Guo, F., Xu, T., Gong, C., Cheng, P., Zhao, L., Cheng, W., & Xu, K. (2014). Evaluating the Role of PTH in Promotion of Chondrosarcoma Cell Proliferation and Invasion by Inhibiting Primary Cilia Expression. International Journal of Molecular Sciences, 15(11), 19816-19831. https://doi.org/10.3390/ijms151119816