Sodium Hydrosulfide Prevents Myocardial Dysfunction through Modulation of Extracellular Matrix Accumulation and Vascular Density
Abstract
:1. Introduction
2. Results and Discussion
2.1. NaHS Inhibited Fibrosis in the Border Zone of Infarcted Myocardium
2.2. NaHS Mitigated Type I and III Collagen as well as Matrix Metalloproteinases-9 (MMP-9) Expression
2.3. NaHS Modulated CSE and Heme Oxygenase-1 Expression in the Border Zone of Infarcted Myocardium
2.4. NaHS Promoted the Growth of New Vessels in the Border Zone of Infarcted Myocardium
2.5. NaHS Prevented Cardiac Dysfunction
Parameters | Sham (n = 7) | Vehicle (n = 8) | NaHS (n = 8) |
---|---|---|---|
LVIDd (cm) | 0.57 ± 0.02 | 0.73 ± 0.03 # | 0.71 ± 0.03 |
LVIDs (cm) | 0.3 ± 0.02 | 0.51 ± 0.06 # | 0.41 ± 0.05 * |
R-R (s) | 0.15 ± 0.01 | 0.15 ± 0.01 | 0.16 ± 0.01 |
LVEDV (mL) | 0.42 ± 0.04 | 0.88 ± 0.05 # | 0.81 ± 0.14 |
LVESV (mL) | 0.07 ± 0.01 | 0.35 ± 0.06 # | 0.19 ± 0.05 * |
EF (%) | 84.0 ± 0.30 | 63.0 ± 0.20 # | 78.0 ± 0.30 * |
FS (%) | 47.37 ± 3.44 | 28.55 ± 1.17 # | 39.45 ± 3.11 * |
HR (bpm) | 393 ± 16 | 415 ± 16 | 396 ± 13 |
2.6. Discussion
3. Methods
3.1. Animal Care
3.2. Chemicals and Antibodies
3.3. Generation of Chronic MI and Drug Treatment
3.4. Echocardiography
3.5. Sample Preparation for Histological and Morphometric Analysis
3.6. Immunohistochemistry and Immunofluorescence Analysis
3.7. Western Blot Analysis
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Abbreviation
CSE | cystathionine γ-lyase |
MI | myocardial infarction |
MMPs | matrix metalloproteinases |
HO-1 | heme oxygenase-1 |
VEGF | vascular endothelial growth factor |
NaHS | Sodium hydrosulfide |
H2S | Hydrogen sulfide |
LVIDd | left ventricular internal dimension diastole |
LVIDs | left ventricular internal dimension systole |
LVEDV | left ventricular end-diastolic volume |
LVESV | left ventricular end-systolic volume |
EF | ejection fraction |
FS | fractional shortening |
Conflicts of Interest
References
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Pan, L.-L.; Wang, X.-L.; Wang, X.-L.; Zhu, Y.-Z. Sodium Hydrosulfide Prevents Myocardial Dysfunction through Modulation of Extracellular Matrix Accumulation and Vascular Density. Int. J. Mol. Sci. 2014, 15, 23212-23226. https://doi.org/10.3390/ijms151223212
Pan L-L, Wang X-L, Wang X-L, Zhu Y-Z. Sodium Hydrosulfide Prevents Myocardial Dysfunction through Modulation of Extracellular Matrix Accumulation and Vascular Density. International Journal of Molecular Sciences. 2014; 15(12):23212-23226. https://doi.org/10.3390/ijms151223212
Chicago/Turabian StylePan, Li-Long, Xian-Li Wang, Xi-Ling Wang, and Yi-Zhun Zhu. 2014. "Sodium Hydrosulfide Prevents Myocardial Dysfunction through Modulation of Extracellular Matrix Accumulation and Vascular Density" International Journal of Molecular Sciences 15, no. 12: 23212-23226. https://doi.org/10.3390/ijms151223212