Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of NP Protein
2.2. Identification of AA Substitution of R452K
2.3. Identification of AA Substitution T423A and N430T
2.4. Bioinformatics Analysis
3. Experimental Section
3.1. Chemicals and Materials
3.2. Virus Cultivation and Purification
3.3. SDS-PAGE
3.4. In-Gel Digestion
3.5. Capillary LC-MS/MS Analysis
3.6. Bioinformatics Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Peptide No. | Peptide sequence | Charge status | Calculated m/z (monoisotopic) | Measured m/z (monoisotopic) | Residues |
---|---|---|---|---|---|
P1 | TGGPIYR | 2 | 382.21 | 382.21 | 92–98 |
P2 | KTGGPIYR | 2 | 446.26 | 446.29 | 91–98 |
P3 | AMMDQVR | 2 | 441.69 | 441.70 | 237–243 |
P4 | QNATEIR | 2 | 416.22 | 416.22 | 20–26 |
P5 | GVFELSDEK | 2 | 512.25 | 512.26 | 462–470 |
P6 | MVLSAFDER | 2 | 534.26 | 534.27 | 66–74 |
P7 | YLEEHPSAGK | 2 | 565.78 | 565.78 | 78–87 |
P8 | YLEEHPSAGKDPK | 2 | 735.86 | 735.86 | 78–90 |
P9 | LIQNSLTIER | 2 | 593.84 | 593.85 | 56–65 |
P10 | GVGTMVMELVR | 2 | 612.31 | 612.32 | 185–195 |
P11 | MVLSAFDERR | 2 | 620.31 | 620.31 | 66–75 |
P12 | EGYSLVGIDPFR | 2 | 676.85 | 676.85 | 294–305 |
P13 | MCSLMQGSTLPR | 2 | 706.82 | 706.82 | 163–174 |
P14 | SYEQMETDGER | 2 | 680.77 | 680.77 | 9–19 |
P15 | ELILYDKEEIR | 2 | 710.89 | 710.89 | 107–117 |
P16 | GVQIASNENMETMESSTLELR | 2 | 1170.05 | 1170.07 | 362–382 |
P17 | SCLPACVYGPAVASGYDFER | 2 | 1110.00 | 1110.00 | 274–293 |
P18 | SQLVWMACHSAAFEDLR | 2 | 1010.97 | 1010.98 | 326–342 |
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Li, Z.; Sun, W.; Wu, D.; Gao, X.; Sun, N.; Liu, N. Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus. Int. J. Mol. Sci. 2014, 15, 2465-2474. https://doi.org/10.3390/ijms15022465
Li Z, Sun W, Wu D, Gao X, Sun N, Liu N. Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus. International Journal of Molecular Sciences. 2014; 15(2):2465-2474. https://doi.org/10.3390/ijms15022465
Chicago/Turabian StyleLi, Zijian, Wanchun Sun, Donglin Wu, Xiang Gao, Ningning Sun, and Ning Liu. 2014. "Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus" International Journal of Molecular Sciences 15, no. 2: 2465-2474. https://doi.org/10.3390/ijms15022465