Intratumoral Decorin Gene Delivery by AAV Vector Inhibits Brain Glioblastomas and Prolongs Survival of Animals by Inducing Cell Differentiation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Induction of U87MG Cell Differentiation by dsAAV CMV-Decorin Vector
2.2. Long-Term Survival of Glioma-Bearing Nude Mice Treated with AAV-Decorin
2.3. Proteome Profiles of 2-DE in U87MG Cells from U87MG, U87MG + AAV-Decorin and U87MG + AAV-EGFP Groups
2.4. Identification of AAV-Decorin-Regulated Proteins Using Peptide Mass Fingerprint (PMF)
2.4.1. Proteins as Apoptosis Mediator
2.4.2. Proteins for Transcription Regulation
2.4.3. Proteins Responsible for Chemotherapy Resistance
2.4.4. Proteins Regulating Cytoskeleton Organization and Mitosis
2.4.5. Proteins Involved in Fatty-Acid Metabolism and Fatty-Acid Modification of Protein
2.5. Multi-Connections of Decorin with Various Biochemical Mechanisms Critical to GBM Biology
2.6. Discussion
3. Materials and Methods
3.1. Construction and Production of dsAAV2 Vector
3.2. Cell Culture, Decorin Gene Transduction and Proliferation Assay
3.3. Animal Study
3.4. Western Blot
3.5. Sample Preparation and 2-DE
3.6. Spot Detection, Quantification, and Comparisons
3.7. Digestion of Proteins
3.8. MALDI-TOF MS
3.9. Statistics
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Spot No. | MOWSE score | Accession No. | Protein name abbreviation | Protein name | Theoretical Mr(kDa)/pI | Observed Mr (kDa)/pI | Matching peptides | Sequence coverage (%) | Change direction |
---|---|---|---|---|---|---|---|---|---|
1 | 18471 | P51398 | DAP3 | Death associated protein 3 | 45.5/9.0 | 49/5.9 | 8 | 20.4 | up |
2 | 1384 | Q9NPJ6 | MED4 | Mediator of RNA polymerase II transcription subunit 4 | 29.7/5.0 | 34/7.2 | 5 | 23.7 | up |
3 | 2803 | P60174 | TPI | Triosephosphate isomerase | 26.5/6.5 | 25/7.4 | 5 | 26.6 | down |
4 | 249 | Q5HYX6 | SHOX | Short stature homeobox | 25.5/6.3 | 20/7.4 | 4 | 17.3 | up |
5 | 265502 | P04264 | KRT1 | Keratin, type II cytoskeletal 1 | 65.8/8.2 | 62/6.1 | 14 | 33 | down |
6 | 11999 | P21281 | V-ATPase | V-type proton ATPase subunit B, brain isoform | 56.5/5.6 | 54/6.1 | 9 | 18.4 | down |
7 | 5656 | P48637 | GSS | Glutathione synthetase | 52.3/5.7 | 50/6.1 | 10 | 25.7 | down |
8 | 25712 | P04181 | OAT | Ornithine aminotransferase, mitochondrial | 48.5/6.6 | 45/6.7 | 9 | 25.1 | down |
9 | 4120 | P40121 | CAPG | Macrophage-capping protein | 38.5/5.9 | 39/6.4 | 8 | 30.2 | down |
10 | 111 | O14832 | PAHX | Phytanoyl-CoA dioxygenase, peroxisomal | 38.5/8.7 | 35/6.1 | 7 | 19.2 | down |
11 | 360 | O75608 | APT1 | Acyl-protein thioesterase 1 | 24.6/6.3 | 26/6.5 | 6 | 28.7 | down |
12 | 266 | Q9BW85 | CCDC94 | Coiled-coil domain-containing protein 94 | 37/5.8 | 35/5.9 | 4 | 21.4 | up |
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Ma, H.-I.; Hueng, D.-Y.; Shui, H.-A.; Han, J.-M.; Wang, C.-H.; Lai, Y.-H.; Cheng, S.-Y.; Xiao, X.; Chen, M.-T.; Yang, Y.-P. Intratumoral Decorin Gene Delivery by AAV Vector Inhibits Brain Glioblastomas and Prolongs Survival of Animals by Inducing Cell Differentiation. Int. J. Mol. Sci. 2014, 15, 4393-4414. https://doi.org/10.3390/ijms15034393
Ma H-I, Hueng D-Y, Shui H-A, Han J-M, Wang C-H, Lai Y-H, Cheng S-Y, Xiao X, Chen M-T, Yang Y-P. Intratumoral Decorin Gene Delivery by AAV Vector Inhibits Brain Glioblastomas and Prolongs Survival of Animals by Inducing Cell Differentiation. International Journal of Molecular Sciences. 2014; 15(3):4393-4414. https://doi.org/10.3390/ijms15034393
Chicago/Turabian StyleMa, Hsin-I, Dueng-Yuan Hueng, Hao-Ai Shui, Jun-Ming Han, Chi-Hsien Wang, Ying-Hsiu Lai, Shi-Yuan Cheng, Xiao Xiao, Ming-Teh Chen, and Yi-Ping Yang. 2014. "Intratumoral Decorin Gene Delivery by AAV Vector Inhibits Brain Glioblastomas and Prolongs Survival of Animals by Inducing Cell Differentiation" International Journal of Molecular Sciences 15, no. 3: 4393-4414. https://doi.org/10.3390/ijms15034393