LXXLL Peptide Converts Transportan 10 to a Potent Inducer of Apoptosis in Breast Cancer Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Cellular Toxicity of TP10-SRC1LXXLL Peptide
2.1.2. Bioportide TP10-SRC1LXXLL Translocates to the Nucleus of MCF-7 Cells
2.1.3. TP10-SRC1LXXLL Affects Viability of Breast Cancer Cells Independently of ER-Mediated Transcription
2.1.4. Effect of TP10-SRC1LXXLL on Apoptosis
2.2. Discussion
3. Experimental Section
3.1. Peptide Synthesis, Purification and Analysis
3.2. Cell Culture
3.3. Viability Assay
3.4. Proliferation Assay
3.5. Quantitative RT-PCR
3.6. Confocal Microscopy
3.7. Detection of Apoptosis with Annexin V and 7-Amino-Actinomycin D (7-AAD) Staining
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
- Author ContributionsK.T. developed the methods and performed optimization experiments, analyzed data, and co-wrote the manuscript. M.P. performed cell culture-related experiments. T.N. and K.P. conceived the methods. K.P. supervised the project and co-wrote the manuscript.
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Abbreviation | Sequence |
---|---|
TP10 | NLS-AGYLLGKINLKALAALAKKIL |
R7 | NLS-RRRRRRR |
TP10-SRC1LXXLL | NLS-AGYLLGKINLKALAALAKKIL-YSQTSHKLVQLLTTAEQQ |
R7-SRC1LXXLL | NLS-RRRRRRR-YSQTSHKLVQLLTTAEQQ |
TP10-SRC11222–1245 | NLS-AGYLLGKINLKALAALAKKIL-PQMQQNVFQYPGAGMVPQGEANF |
R7-SRC11222–1245 | NLS-RRRRRRR-PQMQQNVFQYPGAGMVPQGEANF |
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Tints, K.; Prink, M.; Neuman, T.; Palm, K. LXXLL Peptide Converts Transportan 10 to a Potent Inducer of Apoptosis in Breast Cancer Cells. Int. J. Mol. Sci. 2014, 15, 5680-5698. https://doi.org/10.3390/ijms15045680
Tints K, Prink M, Neuman T, Palm K. LXXLL Peptide Converts Transportan 10 to a Potent Inducer of Apoptosis in Breast Cancer Cells. International Journal of Molecular Sciences. 2014; 15(4):5680-5698. https://doi.org/10.3390/ijms15045680
Chicago/Turabian StyleTints, Kairit, Madis Prink, Toomas Neuman, and Kaia Palm. 2014. "LXXLL Peptide Converts Transportan 10 to a Potent Inducer of Apoptosis in Breast Cancer Cells" International Journal of Molecular Sciences 15, no. 4: 5680-5698. https://doi.org/10.3390/ijms15045680