Gentamicin Blocks the ACh-Induced BK Current in Guinea Pig Type II Vestibular Hair Cells by Competing with Ca2+ at the l-Type Calcium Channel
Abstract
:1. Introduction
2. Results
2.1. GM Reversibly Blocked the ACh-Induced BK Current in Guinea Pig VHCs II in a Dose-Dependent and Voltage-Independent Manner
2.2. Inhibition of GM Is not Affected by ACh Concentration
2.3. Increasing the Extracellular Ca2+ Concentration Antagonizes GM Inhibition and GM Can Block Ca2+ Evoked by (−)-Bay-K8644
2.4. BK Current Evoked by NS1619 Insensitive to 50 μM GM and Only Slightly Blocked by 300 μM GM
3. Discussion
4. Methods
4.1. Ethics Statement
4.2. Animal Procedures and VHCs II Preparation
4.3. Electrophysiology
4.4. Drug Application
4.5. Data Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Yu, H.; Guo, C.-K.; Wang, Y.; Zhou, T.; Kong, W.-J. Gentamicin Blocks the ACh-Induced BK Current in Guinea Pig Type II Vestibular Hair Cells by Competing with Ca2+ at the l-Type Calcium Channel. Int. J. Mol. Sci. 2014, 15, 6757-6771. https://doi.org/10.3390/ijms15046757
Yu H, Guo C-K, Wang Y, Zhou T, Kong W-J. Gentamicin Blocks the ACh-Induced BK Current in Guinea Pig Type II Vestibular Hair Cells by Competing with Ca2+ at the l-Type Calcium Channel. International Journal of Molecular Sciences. 2014; 15(4):6757-6771. https://doi.org/10.3390/ijms15046757
Chicago/Turabian StyleYu, Hong, Chang-Kai Guo, Yi Wang, Tao Zhou, and Wei-Jia Kong. 2014. "Gentamicin Blocks the ACh-Induced BK Current in Guinea Pig Type II Vestibular Hair Cells by Competing with Ca2+ at the l-Type Calcium Channel" International Journal of Molecular Sciences 15, no. 4: 6757-6771. https://doi.org/10.3390/ijms15046757