Chrysin Suppressed Inflammatory Responses and the Inducible Nitric Oxide Synthase Pathway after Spinal Cord Injury in Rats
Abstract
:1. Introduction
2. Results and Disscussion
2.1. CH (Chrysin) Improves the Recovery of Neurological Function and Diminishes the Water Content of Spinal Cord after SCI (Spinal Cord Injury)
Animal Groups | n | 24 h | 48 h | 72 h |
---|---|---|---|---|
Sham | 10 | 16.54 ± 0.87 | 17.24 ± 1.12 | 19.18 ± 1.32 |
SCI | 10 | 4.15 ± 0.67 ** | 3.87 ± 0.92 ** | 4.12 ±0.33 ** |
MPSS | 10 | 12.83 ± 0.55 ## | 13.12 ± 0.87 ## | 14.21 ± 0.66 ## |
CH (30 mg/kg) | 10 | 8.79 ± 0.35 ## | 9.12 ± 0.66 ## | 10.05 ± 0.86 ## |
CH (100 mg/kg) | 10 | 9.05 ± 0.64 ## | 9.85 ± 0.83 ## | 10.11 ± 1.22 ## |
2.2. CH Suppresses Inflammation after SCI
2.3. CH Decreases the Protein Expression and Activity of iNOS (Inducible Nitric Oxide Synthase) and Plasma NO (Nitric Oxide) Concentration after SCI
2.4. CH Inhibits the Caspase-3 Activity after SCI
2.5. Discussion
3. Experimental Section
3.1. Animals
3.2. Drugs and Chemicals
3.3. Induction of SCI Rat Model and Drug Treatment
3.4. Evaluation of Neuronal Function Recovery
3.5. Assessment of Water Content in Spinal Cord Tissue
3.6. Measurement of Serum NF-κB p65 Unit, TNF-α, IL-1β and IL-6 Levels
3.7. Western Blot Analysis
3.8. Determination of iNOS Activity
3.9. Assay of Plasma NO Production
3.10. Measurement of Caspase-3 Activity in Spinal Cord Tissues
3.11. Statistical Analysis
4. Conclusions
Author Contributions
Conflicts of interest
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Jiang, Y.; Gong, F.-L.; Zhao, G.-B.; Li, J. Chrysin Suppressed Inflammatory Responses and the Inducible Nitric Oxide Synthase Pathway after Spinal Cord Injury in Rats. Int. J. Mol. Sci. 2014, 15, 12270-12279. https://doi.org/10.3390/ijms150712270
Jiang Y, Gong F-L, Zhao G-B, Li J. Chrysin Suppressed Inflammatory Responses and the Inducible Nitric Oxide Synthase Pathway after Spinal Cord Injury in Rats. International Journal of Molecular Sciences. 2014; 15(7):12270-12279. https://doi.org/10.3390/ijms150712270
Chicago/Turabian StyleJiang, Yong, Fu-Liang Gong, Guang-Ben Zhao, and Jie Li. 2014. "Chrysin Suppressed Inflammatory Responses and the Inducible Nitric Oxide Synthase Pathway after Spinal Cord Injury in Rats" International Journal of Molecular Sciences 15, no. 7: 12270-12279. https://doi.org/10.3390/ijms150712270