Diurnal Profiles of Melatonin Synthesis-Related Indoles, Catecholamines and Their Metabolites in the Duck Pineal Organ
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characteristics of Chromatographic Assays
Peak No. | Compound | Intra-Day Precision | Inter-Day Precision | ||
---|---|---|---|---|---|
Retention Time MIN–MAX | Peak Area RSD (%) | Retention Time MIN–MAX | Peak Area RSD (%) | ||
1 | 5-HTRP | 3.453–3.455 | 1.8 | 3.451–3.470 | 2.2 |
2 | 5-HT | 4.240–4.244 | 1.9 | 4.240–4.248 | 2.5 |
3 | TRP | 6.295–6.297 | 2.2 | 2.293–6.306 | 2.8 |
4 | 5-HIAA | 7.654–7.656 | 2.4 | 7.650–7.770 | 3.2 |
5 | 5-HTOL | 9.120–9.145 | 2.7 | 9.100–9.157 | 3.7 |
6 | NAS | 14.200–14.203 | 2.4 | 14.192–14.205 | 2.6 |
7 | 5-MTAM | 15.200–15.208 | 2.1 | 15.197–15.230 | 3.2 |
8 | 5-MIAA | 21.270–21.314 | 2.4 | 21.240–21.327 | 3.5 |
9 | 5-MTOL | 23.057–23.067 | 2.8 | 23.054–23.087 | 3.4 |
10 | MLT | 24.604–24.607 | 2.5 | 24.600–24.630 | 3.7 |
Peak No. | Compound | Parameters | Indole Added (pg on Column) | |||
---|---|---|---|---|---|---|
25 | 50 | 250 | 500 | |||
1 | 5-HTRP | Mean recovery (%) | 102.3 | 99.2 | 100.2 | 101.2 |
RSD (%) | 3.2 | 2.2 | 1.8 | 2.0 | ||
2 | 5-HT | Mean recovery (%) | 99.8 | 101.2 | 102.3 | 99.2 |
RSD (%) | 2.8 | 3.2 | 2.8 | 2.9 | ||
3 | TRP | Mean recovery (%) | 102.2 | 99.8 | 100.2 | 99.8 |
RSD (%) | 3.4 | 2.5 | 2.2 | 3.2 | ||
4 | 5-HIAA | Mean recovery (%) | 98.8 | 100.6 | 100.5 | 99.8 |
RSD (%) | 2.9 | 3.2 | 1.9 | 2.8 | ||
5 | 5-HTOL | Mean recovery (%) | 102.4 | 100.1 | 100.2 | 98.7 |
RSD (%) | 1.7 | 2.2 | 1.9 | 2.4 | ||
6 | NAS | Mean recovery (%) | 99.8 | 101.7 | 98.8 | 102.3 |
RSD (%) | 2.1 | 1.2 | 1.2 | 2.4 | ||
7 | 5-MTAM | Mean recovery (%) | 99.7 | 100.1 | 101.4 | 102.4 |
RSD (%) | 1.7 | 2.4 | 2.7 | 2.8 | ||
8 | 5-MIAA | Mean recovery (%) | 102.1 | 101.2 | 102.3 | 99.8 |
RSD (%) | 2.7 | 2.7 | 2.6 | 2.4 | ||
9 | 5-MTOL | Mean recovery (%) | 101.7 | 98.5 | 99.5 | 97.5 |
RSD (%) | 1.9 | 2.3 | 2.4 | 3.2 | ||
10 | MLT | Mean recovery (%) | 103.2 | 100.1 | 1002 | 99.5 |
RSD (%) | 2.6 | 2.1 | 1.9 | 2.4 |
2.2. Pineal Indoles—Diurnal Rhythms and Quantitative Relations
2.2.1. Serotonin and Its Precursors
2.2.2. Direct Derivatives of Serotonin
2.2.3. Melatonin and Other 5-Methoxyindoles
2.3. Catecholamines and Their Metabolites
3. Experimental Section
3.1. Animal Experimental Procedures
3.2. Analytical Procedures
3.2.1. Chemicals
3.2.2. Sample Preparation for Indole and Catecholamine Assays
3.2.3. Indole Assay
3.2.4. Validation of the Indole Assay
3.2.5. Assay of Catecholamines and Their Metabolites
3.2.6. Protein Assay
3.3. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References and Notes
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Lewczuk, B.; Ziółkowska, N.; Prusik, M.; Przybylska-Gornowicz, B. Diurnal Profiles of Melatonin Synthesis-Related Indoles, Catecholamines and Their Metabolites in the Duck Pineal Organ. Int. J. Mol. Sci. 2014, 15, 12604-12630. https://doi.org/10.3390/ijms150712604
Lewczuk B, Ziółkowska N, Prusik M, Przybylska-Gornowicz B. Diurnal Profiles of Melatonin Synthesis-Related Indoles, Catecholamines and Their Metabolites in the Duck Pineal Organ. International Journal of Molecular Sciences. 2014; 15(7):12604-12630. https://doi.org/10.3390/ijms150712604
Chicago/Turabian StyleLewczuk, Bogdan, Natalia Ziółkowska, Magdalena Prusik, and Barbara Przybylska-Gornowicz. 2014. "Diurnal Profiles of Melatonin Synthesis-Related Indoles, Catecholamines and Their Metabolites in the Duck Pineal Organ" International Journal of Molecular Sciences 15, no. 7: 12604-12630. https://doi.org/10.3390/ijms150712604