Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O-Methyltransferase SpOMT2884 from Streptomyces peucetius
Abstract
:1. Introduction
2. Results and Discussion
2.1. Expression of SpOMT2884 in Escherichia coli
2.2. Biotransformation of 8-Hydroxydaidzein by the Recombinant E. coli
2.3 Inhibitory Activity on Melanogenesis of B16 Cells
3. Materials and Methods
3.1. Microorganisms, Cells and Chemicals
3.2. Expression of SpOMT2884 in E. coli
3.3. Fermentation and UPLC
3.4. Scale-up Fermentation, Isolation and Identification of Biotransformation Products
3.5. Determination of Cell Viability and Melanin Content
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Chiang, C.-M.; Ding, H.-Y.; Tsai, Y.-T.; Chang, T.-S. Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O-Methyltransferase SpOMT2884 from Streptomyces peucetius. Int. J. Mol. Sci. 2015, 16, 27816-27823. https://doi.org/10.3390/ijms161126070
Chiang C-M, Ding H-Y, Tsai Y-T, Chang T-S. Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O-Methyltransferase SpOMT2884 from Streptomyces peucetius. International Journal of Molecular Sciences. 2015; 16(11):27816-27823. https://doi.org/10.3390/ijms161126070
Chicago/Turabian StyleChiang, Chien-Min, Hsiou-Yu Ding, Ya-Ting Tsai, and Te-Sheng Chang. 2015. "Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O-Methyltransferase SpOMT2884 from Streptomyces peucetius" International Journal of Molecular Sciences 16, no. 11: 27816-27823. https://doi.org/10.3390/ijms161126070