Bone-Remodeling Transcript Levels Are Independent of Perching in End-of-Lay White Leghorn Chickens
Abstract
:1. Introduction
2. Results
Transcript | Primer Pair (5' to 3') | Amplified NT Location (Amplicon Size) | Accession Number |
---|---|---|---|
GAPDH | F: GCACCACCAACTGCCTGGCACCCTTG | 425–634 (210) | ENSGALT00000023323 |
R: GGATGACTTTCCCCACAGCCTTAGCAGC | |||
COL2A1 | F: CATCCTCATCCAGGGATCCAAC | 3533–3733 (200) | AF452711 |
R: ACTCCTGATCGGCTCCGCCAATGT | |||
SOX9 | F: AAATGACAGAAGAACAGGACAAATG | 293–585 (293) | ENSGALT00000038513 |
R: CTTCACGTGGGGTTTGTTCTTG | |||
PTHLH | F: TCAGAGCACCAGCTACTGCATGAC | 136–286 (151) | NM_205338 |
R: CCTAAGCCTGCTACCAACACAA | |||
PTH1R | F: GCACTAGAAACTACATCCACATGC | 521–754 (234) | DQ914925.1 |
R: AGTAGTAATTGGTTGCCAGGAAGT | |||
PTH3R | F: ACCAGCTGCCTCCCAGAATGGGAT | 111–204 (94) | XM_425837 |
R: TGAAGTCGTAGATGTAGTCAGGGC | |||
OPG | F: AGACTGGAACAGCAACGACGAG | 272–470 (199) | ENSGALT00000030811 |
R: GACAGACTGCTTTGGATGACGT | |||
RANKL | F: AGGAGGTGAAGTTAATGCCAGAAT | 101–398 (298) | ENSGALT00000027412 |
R: AGTTTCCCATCACTGAACGTCATA |
Transcript | Treatment | Bone | Interaction |
---|---|---|---|
RANKL/OPG RATIO | F3,27 = 1.81, p = 0.17 | F2,64 = 1.48, p = 0.25 | F6,64 = 1.09, p = 0.39 |
SOX9 | F3,32 = 0.24, p = 0.87 | F2,64 = 1.12, p = 0.34 | F6,64 = 0.76, p = 0.61 |
PTH1R | F3,32 = 0.59, p = 0.63 | F2,64 = 2.47, p = 0.10 | F6,64 = 0.92, p = 0.50 |
PTH3R | F3,32 = 0.49, p = 0.70 | F2,64 = 0.49, p = 0.61 | F6,64 = 1.13, p = 0.37 |
PTHLH | F3,32 = 0.22, p = 0.88 | F2,64 = 0.07, p = 0.94 | F6,64 = 1.25, p = 0.30 |
COL2A1 | F3,32 = 1.73, p = 0.18 | F2,64 = 4.25, p = 0.02 | F6,64 = 0.96, p = 0.46 |
Phalange | Tibia | Femur |
---|---|---|
A 0.54 ± 0.06 | AB 0.68 ± 0.06 | B 0.79 ± 0.10 |
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Total RNA Isolation and cDNA Synthesis
4.3. Real-Time Quantitative PCR and Cloning cDNA for Sequence Validation
4.4. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Appendix
Treatment Bone | Control | Perch during Laying | Perch during Pullet | Perch during Pullet and Laying | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
F | P | T | F | P | T | F | P | T | F | P | T | |
SOX9 | 0.45 ± 0.99 | 4.29 ± 3.13 | 0.90 ± 0.74 | 1.65 ± 0.99 | 5.96 ± 3.13 | 0.59 ± 0.74 | 0.77 ± 0.99 | 1.41 ± 3.13 | 1.68 ± 0.74 | 1.75 ± 0.99 | 0.58 ± 3.13 | 1.71 ± 0.74 |
PTHLH | 0.75 ± 0.69 | 1.30 ± 0.58 | 0.38 ± 0.84 | 1.77 ± 0.69 | 0.46 ± 0.58 | 0.64 ± 0.84 | 0.25 ± 0.69 | 0.55 ± 0.58 | 0.53 ± 0.84 | 0.10 ± 0.69 | 0.25 ± 0.58 | 1.67 ± 0.84 |
PTH1R | 0.43 ± 0.15 | 1.52 ± 0.64 | 0.30 ± 0.75 | 0.38 ± 0.15 | 0.80 ± 0.64 | 1.25 ± 0.75 | 0.18 ± 0.15 | 0.24 ± 0.64 | 1.89 ± 0.75 | 0.14 ± 0.15 | 0.16 ± 0.64 | 0.40 ± 0.75 |
PTH3R | 1.76 ± 0.68 | 2.99 ± 1.20 | 0.91 ± 0.67 | 1.85 ± 0.68 | 2.23 ± 0.64 | 1.41 ± 0.67 | 0.68 ± 0.68 | 1.28 ± 1.20 | 1.41 ± 0.67 | 0.64 ± 0.68 | 0.56 ± 1.20 | 2.31 ± 0.67 |
OPG | 0.76 ± 1.09 | 0.18 ± 0.33 | 0.39 ± 0.39 | 0.24 ± 1.16 | 0.70 ± 0.39 | 0.19 ± 0.41 | 1.82 ± 1.03 | 0.76 ± 0.33 | 0.94 ± 0.37 | 1.42 ± 1.03 | 0.08 ± 0.32 | 0.68 ± 0.36 |
RANKL | 1.09 ± 3.85 | 0.07 ± 0.09 | 0.23 ± 0.24 | 7.75 ± 3.84 | 0.34 ± 0.11 | 0.26 ± 0.30 | −0.66 ± 4.50 | 0.25 ± 0.09 | 0.51 ± 0.25 | 0.45 ± 3.60 | 0.30 ± 0.09 | 0.53 ± 0.24 |
RANKL/OPG RATIO | 0.23 ± 4.24 | −0.04 ± 0.42 | 0.02 ± 0.53 | 9.73 ± 4.15 | −0.62 ± 0.61 | 0.05 ± 0.73 | −2.67 ± 4.77 | −0.62 ± 0.40 | 0.36 ± 0.57 | −1.49 ± 3.79 | −0.19 ± 0.41 | 0.41 ± 0.51 |
COL2A1 | 0.92 ± 0.19 | 0.59 ± 0.11 | 0.68 ± 0.13 | 1.02 ± 0.19 | 0.45 ± 0.11 | 0.69 ± 0.13 | 0.53 ± 0.19 | 0.39 ± 0.11 | 0.51 ± 0.13 | 0.68 ± 0.19 | 0.74 ± 0.11 | 0.84 ± 0.13 |
Conflicts of Interest
References
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Dale, M.D.; Mortimer, E.M.; Kolli, S.; Achramowicz, E.; Borchert, G.; Juliano, S.A.; Halkyard, S.; Sietz, N.; Gatto, C.; Hester, P.Y.; et al. Bone-Remodeling Transcript Levels Are Independent of Perching in End-of-Lay White Leghorn Chickens. Int. J. Mol. Sci. 2015, 16, 2663-2677. https://doi.org/10.3390/ijms16022663
Dale MD, Mortimer EM, Kolli S, Achramowicz E, Borchert G, Juliano SA, Halkyard S, Sietz N, Gatto C, Hester PY, et al. Bone-Remodeling Transcript Levels Are Independent of Perching in End-of-Lay White Leghorn Chickens. International Journal of Molecular Sciences. 2015; 16(2):2663-2677. https://doi.org/10.3390/ijms16022663
Chicago/Turabian StyleDale, Maurice D., Erin M. Mortimer, Santharam Kolli, Erik Achramowicz, Glenn Borchert, Steven A. Juliano, Scott Halkyard, Nick Sietz, Craig Gatto, Patricia Y. Hester, and et al. 2015. "Bone-Remodeling Transcript Levels Are Independent of Perching in End-of-Lay White Leghorn Chickens" International Journal of Molecular Sciences 16, no. 2: 2663-2677. https://doi.org/10.3390/ijms16022663