The Role of hOGG1 C1245G Polymorphism in the Susceptibility to Lupus Nephritis and Modulation of the Plasma 8-OHdG in Patients with Systemic Lupus Erythematosus †
Abstract
:1. Introduction
2. Results and Discussion
2.1. Distributions of hOGG1 C1245G Polymorphism in Healthy Controls and SLE Patients
2.2. Levels of Plasma 8-OHdG in Healthy Controls and SLE Patients Based on the hOGG1 Gene Polymorphisms
Subjects (Case Number, %) | hOGG1 C1245G Polymorphisms | p-Value | |
---|---|---|---|
hOGG1 1245 CC or CG Genotype | hOGG1 GG Genotype | ||
Healthy controls (n = 45, 100) | 21 (46.7)—Group A | 24 (53.3)—Group B | 0.017 * |
SLE patients (n = 85, 100) | 58 (68.2)—Group C | 27 (31.8)—Group D |
Plasma 8-OhdG (M ± SD, ng/mL) | Plasma 8-OHdG (M ± SD, ng/mL) | p-Value | ||
---|---|---|---|---|
Healthy controls (n = 45) | 0.157 ± 0.038 | SLE patients (n = 85) | 0.225 ± 0.082 | <0.001 [20] |
hOGG1 C1245G polymorphisms | hOGG1 C1245G polymorphisms | |||
Group A (CC or CG genotypes, n = 21) | 0.161 ± 0.036 | Group C (CC or CG genotypes, n = 58) | 0.217 ± 0.059 | <0.001 * |
Group B (GG genotype, n = 24) | 0.155 ± 0.041 | Group D (GG genotype, n = 27) | 0.243 ± 0.117 | 0.001 * |
p-value | 0.614 ** | p-value | 0.289 ** | <0.001 *** |
2.3. Distributions of hOGG1 C1245G Polymorphisms in SLE Patients with or without Lupus Nephritis
SLE Patients | hOGG1 Gene Polymorphisms (Case Number, %) | p-Value | |
---|---|---|---|
hOGG1 1245 CC or CG Genotype (n = 58, 100%) | hOGG1 GG Genotype (n = 27, 100%) | ||
Without nephritis | 40 (69.0%)—Group I | 12 (44.4%)—Group II | 0.031 * |
With nephritis | 18 (31.0%)—Group III | 15 (55.6%)—Group IV |
2.4. Plasma Levels of 8-OHdG in SLE Patients as Related to the Clinical Presentations of Lupus Nephritis and hOGG1 C1245G Polymorphism
Plasma 8-OhdG (M ± SD, ng/mL) | Plasma 8-OhdG (M ± SD, ng/mL) | p-Value | ||
---|---|---|---|---|
SLE patients without nephritis (n = 52) | 0.218 ± 0.060 | SLE patients with nephritis (n = 33) | 0.237 ± 0.109 | 0.313 |
Group I hOGG1 1245 CC or CG genotype (n = 40) | 0.224 ± 0.064 | Group III hOGG1 1245 CC or CG genotype (n = 18) | 0.201 ± 0.046 | 0.165 * |
Group II GG genotype (Cys/Cys hOGG1) (n = 12) | 0.197 ± 0.040 | Group IV GG genotype (Cys/Cys hOGG1) (n = 15) | 0.280 ± 0.145 | 0.050 * |
p-value | 0.164 ** | 0.037 ** | 0.054 *** | |
0.020 **** |
3. Experimental Section
3.1. Recruitment of SLE Patients and Healthy Controls
3.2. Blood Sample Collection, Plasma Separation and Leukocyte DNA Preparation
3.3. Determination of 8-OHdG in Plasma by ELISA
3.4. Analysis of hOGG1 C1245G Polymorphisms
3.5 Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lee, H.-T.; Lin, C.-S.; Lee, C.-S.; Tsai, C.-Y.; Wei, Y.-H. The Role of hOGG1 C1245G Polymorphism in the Susceptibility to Lupus Nephritis and Modulation of the Plasma 8-OHdG in Patients with Systemic Lupus Erythematosus. Int. J. Mol. Sci. 2015, 16, 3757-3768. https://doi.org/10.3390/ijms16023757
Lee H-T, Lin C-S, Lee C-S, Tsai C-Y, Wei Y-H. The Role of hOGG1 C1245G Polymorphism in the Susceptibility to Lupus Nephritis and Modulation of the Plasma 8-OHdG in Patients with Systemic Lupus Erythematosus. International Journal of Molecular Sciences. 2015; 16(2):3757-3768. https://doi.org/10.3390/ijms16023757
Chicago/Turabian StyleLee, Hui-Ting, Chen-Sung Lin, Chyou-Shen Lee, Chang-Youh Tsai, and Yau-Huei Wei. 2015. "The Role of hOGG1 C1245G Polymorphism in the Susceptibility to Lupus Nephritis and Modulation of the Plasma 8-OHdG in Patients with Systemic Lupus Erythematosus" International Journal of Molecular Sciences 16, no. 2: 3757-3768. https://doi.org/10.3390/ijms16023757