Tetraspanin 8-Rictor-Integrin α3 Complex Is Required for Glioma Cell Migration
Abstract
:1. Introduction
2. Results
2.1. Over-Expression of Tspan8 in Human Malignant Glioma Tissues and Cell Lines
2.2. Tspan8 Associates with Rictor and Integrin α3 in both Human Glioma Tissues and Cell Lines
2.3. SiRNA-Mediated Knockdown of Tspan8 or Integrin α3 Inhibits U251MG Cell Migration
2.4. Tspan8-Rictor Association Is Required for mTORC2 Activation, AKT Ser-473 Phosphorylation and U251MG Cell Migration
3. Discussion
4. Experimental Section
4.1. Cell Culture and Reagents
4.2. Western Blot Analysis
4.3. Co-Immunoprecipitation (IP)
4.4. Transwell Assay
4.5. MTT Cell Viability Assay
4.6. siRNA
4.7. Patient’s Glioma Tissues Isolation and Preparation
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Pan, S.-J.; Zhan, S.-K.; Pan, Y.-X.; Liu, W.; Bian, L.-G.; Sun, B.; Sun, Q.-F. Tetraspanin 8-Rictor-Integrin α3 Complex Is Required for Glioma Cell Migration. Int. J. Mol. Sci. 2015, 16, 5363-5374. https://doi.org/10.3390/ijms16035363
Pan S-J, Zhan S-K, Pan Y-X, Liu W, Bian L-G, Sun B, Sun Q-F. Tetraspanin 8-Rictor-Integrin α3 Complex Is Required for Glioma Cell Migration. International Journal of Molecular Sciences. 2015; 16(3):5363-5374. https://doi.org/10.3390/ijms16035363
Chicago/Turabian StylePan, Si-Jian, Shi-Kun Zhan, Yi-Xin Pan, Wei Liu, Liu-Guan Bian, Bomin Sun, and Qing-Fang Sun. 2015. "Tetraspanin 8-Rictor-Integrin α3 Complex Is Required for Glioma Cell Migration" International Journal of Molecular Sciences 16, no. 3: 5363-5374. https://doi.org/10.3390/ijms16035363