Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
Abstract
:1. Introduction
2. Results
2.1. Recognition Specificity of BH2
Class I (Positive) | Class II (Positive) |
---|---|
Cw10, B27, B57, B51, B61, B13, B44 | |
B75, B55, Cw4, Cw12, B49, B38, Cw1 | |
B76, B54, Cw16, B8, B48, B35, Cw15 | |
B42, Cw7, Cw9, B41, B7, B62, Cw6 | |
B82, B37, Cw17, B60, B56, B65, B45, B81 | Negative |
B59, B52, B39, Cw2, Cw14, B78, B46 | |
Cw5, Cw8, B72, B58, B63, B77, B73 | |
B71, B64, B53, B67, B47, B18, Cw18 | |
B50, A11 |
2.2. BH2 Binds to the α2 Domain of HLA-B2704 HC
3. Experimental Section
3.1. Materials
3.2. HLA-Typing
3.3. Analysis of BH2-Binding Domain
- Alpha 1 primers: 5'-GGCGGCTCCCACTCCATGAGG-3' and 5'-GGCCTCGCTCTGGTTGATG-3'
- Alpha 2 primers: 5'-GGGTCTCACACCCTCCAGAA-3' and 5'-CGCGCGCTGCAGCGTCTC-3'
- Alpha 3 primers: 5'-GACCCCCCAAAGACACACG-3' and 5'-CCATCTCAGGGTGAGGGG-3'
3.4. Site-Directed Mutagenesis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yu, H.-C.; Huang, K.-Y.; Lu, M.-C.; Huang, H.-L.; Liu, W.-T.; Lee, W.-C.; Liu, S.-Q.; Huang, H.-B.; Lai, N.-S. Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain. Int. J. Mol. Sci. 2015, 16, 8142-8150. https://doi.org/10.3390/ijms16048142
Yu H-C, Huang K-Y, Lu M-C, Huang H-L, Liu W-T, Lee W-C, Liu S-Q, Huang H-B, Lai N-S. Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain. International Journal of Molecular Sciences. 2015; 16(4):8142-8150. https://doi.org/10.3390/ijms16048142
Chicago/Turabian StyleYu, Hui-Chun, Kuang-Yung Huang, Ming-Chi Lu, Hsien-Lu Huang, Wei-Ting Liu, Wen-Chien Lee, Su-Qin Liu, Hsien-Bin Huang, and Ning-Sheng Lai. 2015. "Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain" International Journal of Molecular Sciences 16, no. 4: 8142-8150. https://doi.org/10.3390/ijms16048142