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Article

1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation

by
Soichiro Murata
1,*,
Koichi Ogawa
1,
Takashi Matsuzaka
2,
Mitsuru Chiba
3,
Ken Nakayama
1,
Kenichi Iwasaki
1,
Tomohiro Kurokawa
1,
Naoki Sano
1,
Tomohito Tanoi
1 and
Nobuhiro Ohkohchi
1
1
Department of Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
2
Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
3
Department of Biomedical Sciences, Division of Medical Life Sciences, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036-8564, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2015, 16(6), 12051-12063; https://doi.org/10.3390/ijms160612051
Submission received: 1 April 2015 / Accepted: 15 May 2015 / Published: 27 May 2015
(This article belongs to the Special Issue Molecular Mechanisms of Human Liver Diseases)
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Abstract

Hepatocyte-specific Phosphatase and tensin homolog (Pten)-knockout (KO) mice exhibit hepatic lesions analogous to non-alcoholic steatohepatitis (NASH). 1,8-cineole is a monoterpene oxide and it has several biological effects including hepatoprotective effects. In this study we revealed that 1,8-cineole ameliorates NASH of Pten KO mice. Pten KO mice were assigned to a control group without any medication or to a 1,8-cineole group injected with 50 mg/kg i.p. twice per week for eight weeks. At eight weeks, livers from each group were processed to measure triglyceride (TG) content, gene expression analysis, western blot analysis, and histological examination including Oil red O staining. 1,8-cineole ameliorated hepatic steatosis in Pten KO mice, revealed by TG content and Oil red O staining. Moreover, 1,8-cineole downregulated collagen 1a1 expression and improved liver fibrosis. Thus, 1,8-cineole has potential as a candidate to treat NASH by inactivating the Akt/PI3-kinase pathway.
Keywords: 1,8-cineole; NASH; PTEN; Akt; LXR alpha 1,8-cineole; NASH; PTEN; Akt; LXR alpha

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MDPI and ACS Style

Murata, S.; Ogawa, K.; Matsuzaka, T.; Chiba, M.; Nakayama, K.; Iwasaki, K.; Kurokawa, T.; Sano, N.; Tanoi, T.; Ohkohchi, N. 1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation. Int. J. Mol. Sci. 2015, 16, 12051-12063. https://doi.org/10.3390/ijms160612051

AMA Style

Murata S, Ogawa K, Matsuzaka T, Chiba M, Nakayama K, Iwasaki K, Kurokawa T, Sano N, Tanoi T, Ohkohchi N. 1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation. International Journal of Molecular Sciences. 2015; 16(6):12051-12063. https://doi.org/10.3390/ijms160612051

Chicago/Turabian Style

Murata, Soichiro, Koichi Ogawa, Takashi Matsuzaka, Mitsuru Chiba, Ken Nakayama, Kenichi Iwasaki, Tomohiro Kurokawa, Naoki Sano, Tomohito Tanoi, and Nobuhiro Ohkohchi. 2015. "1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation" International Journal of Molecular Sciences 16, no. 6: 12051-12063. https://doi.org/10.3390/ijms160612051

APA Style

Murata, S., Ogawa, K., Matsuzaka, T., Chiba, M., Nakayama, K., Iwasaki, K., Kurokawa, T., Sano, N., Tanoi, T., & Ohkohchi, N. (2015). 1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation. International Journal of Molecular Sciences, 16(6), 12051-12063. https://doi.org/10.3390/ijms160612051

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