Fenarimol, a Pyrimidine-Type Fungicide, Inhibits Brassinosteroid Biosynthesis
Abstract
:1. Introduction
2. Results
2.1. Fenarimol Induces Dwarfism and De-Etiolate Phenotypes of Arabidopsis Seedlings in the Dark
2.2. Brz-Resistant Mutant Bzr-1-1D/bil1-1D Displays Tolerance to Fenarimol Treatment
2.3. Determination of the Target Site(s) of Fenarimol on BR Biosynthesis Inhibition
2.4. Fenarimol Binds to CYP90D1
2.5. Molecular Function of Fenarimol for the Expression of BR-Responsive Genes
3. Discussion
4. Experimental Section
4.1. Chemicals
4.2. Plant Growth Conditions and BR Biosynthesis Inhibition Assay
4.3. Quantitative Real-Time PCR
4.4. Construction of CYP90D1 Expression Vectors
4.5. Expression and Purification of Recombinant CYP90D1
4.6. Binding Assay of Fenarimol to Recombinant CYP90D1
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Oh, K.; Matsumoto, T.; Yamagami, A.; Hoshi, T.; Nakano, T.; Yoshizawa, Y. Fenarimol, a Pyrimidine-Type Fungicide, Inhibits Brassinosteroid Biosynthesis. Int. J. Mol. Sci. 2015, 16, 17273-17288. https://doi.org/10.3390/ijms160817273
Oh K, Matsumoto T, Yamagami A, Hoshi T, Nakano T, Yoshizawa Y. Fenarimol, a Pyrimidine-Type Fungicide, Inhibits Brassinosteroid Biosynthesis. International Journal of Molecular Sciences. 2015; 16(8):17273-17288. https://doi.org/10.3390/ijms160817273
Chicago/Turabian StyleOh, Keimei, Tadashi Matsumoto, Ayumi Yamagami, Tomoki Hoshi, Takeshi Nakano, and Yuko Yoshizawa. 2015. "Fenarimol, a Pyrimidine-Type Fungicide, Inhibits Brassinosteroid Biosynthesis" International Journal of Molecular Sciences 16, no. 8: 17273-17288. https://doi.org/10.3390/ijms160817273