Comparison of Soybean Transformation Efficiency and Plant Factors Affecting Transformation during the Agrobacterium Infection Process
Abstract
:1. Introduction
2. Results
2.1. Transformation Efficiencies of Various Soybean Cultivars
Cultivar | No. of Explants Infected | No. of Explants with above Three Shoots | Resistant Shoot Induction Rate (%) | No. of Positive T0 Plants | Transformation Efficiency of T0 Plants (%) |
---|---|---|---|---|---|
Liaodou16 | 348 | 11 | 3.2 | 0 | 0 |
General | 481 | 46 | 9.6 | 1 | 0.21 |
Kottman | 432 | 44 | 10.2 | 3 | 0.69 |
Dennison | 348 | 67 | 19.3 | 3 | 0.86 |
Shennong 12 | 504 | 185 | 36.7 | 8 | 1.59 |
Liaodou 14 | 384 | 244 | 63.5 | 7 | 1.82 |
Liaodou 10 | 576 | 382 | 66.3 | 13 | 2.26 |
Bert | 624 | 477 | 76.4 | 32 | 5.13 |
Shennong 9 | 564 | 436 | 77.3 | 30 | 5.32 |
Williams 82 | 492 | 397 | 80.7 | 33 | 6.71 |
2.2. Susceptibility of Different Soybean Genotypes to Agrobacterium Infection
Genotype | No. of Cotyledonary Nodes for GUS Staining | No. of GUS+ Cotyledonary Nodes | GUS+ Rate (%) |
---|---|---|---|
General | 98 | 32 | 32.7 |
Liaodou 16 | 84 | 16 | 19.1 |
Kottman | 100 | 30 | 30.0 |
Williams 82 | 108 | 86 | 85.7 |
Shennong 9 | 98 | 84 | 79.6 |
Bert | 104 | 80 | 76.9 |
2.3. Germination and Growth Related Factors Affecting Transformation
2.4. Cell Division Related Factors Affecting Transformation
2.5. Defense Response Related Factors Affecting Transformation
3. Discussion
4. Experimental Section
4.1. Materials
4.2. Agrobacterium Preparation
4.3. Infection and Co-Cultivation of Explant
4.4. Transgenetic Shoots Induction and Plant Regeneration
4.5. Detection of T0 Plants
4.6. β-Glucuronidase (GUS) Staining of Cotyledonary Node
4.7. BAR Gene Analysis during Co-Cultivation Period
4.8. Measurement of Gibberellin (GA), Abscisic Acid (ABA), Zeatin Riboside (ZR) and Methyl Jasmonate (MeJA) Content
4.9. Assay of Polyphenol Oxidase (PPO) and Peroxidase (POD) Activity
4.10. Expression of Selected Genes
4.11. Measurement of DNA Quantity
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Jia, Y.; Yao, X.; Zhao, M.; Zhao, Q.; Du, Y.; Yu, C.; Xie, F. Comparison of Soybean Transformation Efficiency and Plant Factors Affecting Transformation during the Agrobacterium Infection Process. Int. J. Mol. Sci. 2015, 16, 18522-18543. https://doi.org/10.3390/ijms160818522
Jia Y, Yao X, Zhao M, Zhao Q, Du Y, Yu C, Xie F. Comparison of Soybean Transformation Efficiency and Plant Factors Affecting Transformation during the Agrobacterium Infection Process. International Journal of Molecular Sciences. 2015; 16(8):18522-18543. https://doi.org/10.3390/ijms160818522
Chicago/Turabian StyleJia, Yuying, Xingdong Yao, Mingzhe Zhao, Qiang Zhao, Yanli Du, Cuimei Yu, and Futi Xie. 2015. "Comparison of Soybean Transformation Efficiency and Plant Factors Affecting Transformation during the Agrobacterium Infection Process" International Journal of Molecular Sciences 16, no. 8: 18522-18543. https://doi.org/10.3390/ijms160818522