Identification and Characterization of CYP9A40 from the Tobacco Cutworm Moth (Spodoptera litura), a Cytochrome P450 Gene Induced by Plant Allelochemicals and Insecticides
Abstract
:1. Introduction
2. Results
2.1. Identification of CYP9A40
2.2. Accumulation of CYP9A40 Transcripts during Different Developmental Stages
2.3. Expression of CYP9A40 in Different Tissues
2.4. Induction of CYP9A40 Expression by Plant Allelochemicals and Insecticides
2.5. Silencing of CYP9A40 by RNAi Suggests a Role of CYP9A40 in Detoxification of Plant Allelochemicals and Insecticides
3. Discussion
4. Experimental Section
4.1. Insects
4.2. Cloning of the CYP9A40 Gene
4.3. RNA Extraction and cDNA Synthesis
4.4. Phylogenetic Analysis
4.5. Quantitative Real-Time PCR
4.6. Expression of CYP9A40 during Different Developmental Stages and in Various Tissues
4.7. Expression Analysis of CYP9A40 in Response to Xenobiotics
4.8. Silencing of CYP9A40 by RNAi and Bioassay with Xenobiotics
4.9. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wang, R.-L.; Staehelin, C.; Xia, Q.-Q.; Su, Y.-J.; Zeng, R.-S. Identification and Characterization of CYP9A40 from the Tobacco Cutworm Moth (Spodoptera litura), a Cytochrome P450 Gene Induced by Plant Allelochemicals and Insecticides. Int. J. Mol. Sci. 2015, 16, 22606-22620. https://doi.org/10.3390/ijms160922606
Wang R-L, Staehelin C, Xia Q-Q, Su Y-J, Zeng R-S. Identification and Characterization of CYP9A40 from the Tobacco Cutworm Moth (Spodoptera litura), a Cytochrome P450 Gene Induced by Plant Allelochemicals and Insecticides. International Journal of Molecular Sciences. 2015; 16(9):22606-22620. https://doi.org/10.3390/ijms160922606
Chicago/Turabian StyleWang, Rui-Long, Christian Staehelin, Qing-Qing Xia, Yi-Juan Su, and Ren-Sen Zeng. 2015. "Identification and Characterization of CYP9A40 from the Tobacco Cutworm Moth (Spodoptera litura), a Cytochrome P450 Gene Induced by Plant Allelochemicals and Insecticides" International Journal of Molecular Sciences 16, no. 9: 22606-22620. https://doi.org/10.3390/ijms160922606