The Sequence Characteristics and Expression Models Reveal Superoxide Dismutase Involved in Cold Response and Fruiting Body Development in Volvariella volvacea
Abstract
:1. Introduction
2. Results
2.1. Annotation and Sequence Accuracy Verification of SOD Encoding Genes
2.2. Genetic Structure and Alternative Splicing Analysis
2.3. Alignment and Phylogenetic Analysis of Amino Acid Sequences
2.4. Differential Expression of Sod Genes under Short-Term Cold Stress
2.5. Expression Patterns of Sod Genes during the Fruiting-body Development Stages of V. volvacea
3. Discussion
4. Materials and Methods
4.1. Strains
4.2. Genome and Transcriptome Sequencing Data
4.3. Identification of the SOD Proteins of V. volvacea
4.4. Acquisition, Accuracy Verification and Structural Analysis of Full-Length Gene Sequences
4.5. Bioinformatics Analysis
4.6. Cold Stress Treatment
4.7. Mycelia and Fruiting-Body Growth and Sample Collection
4.8. DNA Extraction, PCR Amplification, and Sequence Determination
Gene | Primer Sequence(5’-3’) | Annealing Temperature for PCR |
---|---|---|
Vv_Cu-Znsod1 | GGTTGCCGTCCTGTCCTTCTT | 58.0 °C |
CGCCTGTAGTATTCTTGCCCTCAT | ||
Vv_Mnsod1 | GACCAACGACCTTGATCCATC | 54.6 °C |
GGACAACCAACGCCTGATG | ||
Vv_Mnsod2 | GGTGAGAGTTGGAAGCGGTA | 54.9 °C |
GGCACTGGACAGAGCGTAT | ||
GME5781 | TTCCTTTACGCTGCCAACT | 55.4 °C |
GTACGGCTGTGAATATGTCGA |
4.9. RNA Extraction and Reverse Transcription
4.10. RT-qPCR Assay of Gene Expression
Gene | Forward Sequence (5’-3’) | Reverse Sequence (5’-3’) |
---|---|---|
Vv_Cu-Znsod1 | ACATTCGTCGCTGCTCTCCT | GTGAAGTTGACTGTGCCTGTGA |
Vv_Mnsod1 | CGTTACCACCGCCAACCAAGA | TTAGGAGAGACCCAACCCTTAAAGC |
Vv_Mnsod2 | TGCTCGCCATCGCCAGAACT | GGTGGTGCTTGGTGTGGTGAAG |
gapdh | CATCTTCCACTGGTGCGGCTAAG | GGCTTCTCAAGGCGAACGACAA |
4.11. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yan, J.-J.; Zhang, L.; Wang, R.-Q.; Xie, B.; Li, X.; Chen, R.-L.; Guo, L.-X.; Xie, B.-G. The Sequence Characteristics and Expression Models Reveal Superoxide Dismutase Involved in Cold Response and Fruiting Body Development in Volvariella volvacea. Int. J. Mol. Sci. 2016, 17, 34. https://doi.org/10.3390/ijms17010034
Yan J-J, Zhang L, Wang R-Q, Xie B, Li X, Chen R-L, Guo L-X, Xie B-G. The Sequence Characteristics and Expression Models Reveal Superoxide Dismutase Involved in Cold Response and Fruiting Body Development in Volvariella volvacea. International Journal of Molecular Sciences. 2016; 17(1):34. https://doi.org/10.3390/ijms17010034
Chicago/Turabian StyleYan, Jun-Jie, Lei Zhang, Rui-Qing Wang, Bin Xie, Xiao Li, Ren-Liang Chen, Li-Xian Guo, and Bao-Gui Xie. 2016. "The Sequence Characteristics and Expression Models Reveal Superoxide Dismutase Involved in Cold Response and Fruiting Body Development in Volvariella volvacea" International Journal of Molecular Sciences 17, no. 1: 34. https://doi.org/10.3390/ijms17010034