MicroRNA-375 Functions as a Tumor-Suppressor Gene in Gastric Cancer by Targeting Recepteur d’Origine Nantais
Abstract
:1. Introduction
2. Results
2.1. miR-375 Directly Targets the 3′-Untranslated Region (3′-UTR) of Recepteur d’Origine Nantais (RON)
2.2. Inverse Association between miR-375 and RON Expression in AGS and MKN-28 Human Gastric Cancer Cells
2.3. miR-375 Inhibits AGS and MKN-28 Human Gastric Cancer Cell Proliferation
2.4. miR-375 Induced G1 Cell Cycle Arrest by Decreasing Cyclin D1 and Cyclin D3 Expression, and Retinoblastoma (Rb) Phosphorylation
2.5. miR-375 Inhibits Migration in AGS and MKN-28 Human Gastric Cancer Cells
2.6. miR-375 Inhibits Invasion in AGS and MKN-28 Human Gastric Cancer Cells
2.7. Knockdown of RON Reduces Proliferation, Migration, and Invasion in AGS and MKN-28 Human Gastric Cancer Cells
3. Discussion
4. Experimental Section
4.1. Reagents
4.2. Cell Culture
4.3. Cell Transfection
4.4. Luciferase Activity Assay
4.5. RNA Extraction and Quantitative Real Time PCR (qRT-PCR)
4.6. Preparation of Cell Extracts and Western Blot Analysis
4.7. Cell Cycle Analysis
4.8. Cell Proliferation Assay
4.9. In Vitro Wound-Healing Assay
4.10. Matrigel Invasion Assay
4.11. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lian, S.; Park, J.S.; Xia, Y.; Nguyen, T.T.; Joo, Y.E.; Kim, K.K.; Kim, H.K.; Jung, Y.D. MicroRNA-375 Functions as a Tumor-Suppressor Gene in Gastric Cancer by Targeting Recepteur d’Origine Nantais. Int. J. Mol. Sci. 2016, 17, 1633. https://doi.org/10.3390/ijms17101633
Lian S, Park JS, Xia Y, Nguyen TT, Joo YE, Kim KK, Kim HK, Jung YD. MicroRNA-375 Functions as a Tumor-Suppressor Gene in Gastric Cancer by Targeting Recepteur d’Origine Nantais. International Journal of Molecular Sciences. 2016; 17(10):1633. https://doi.org/10.3390/ijms17101633
Chicago/Turabian StyleLian, Sen, Jung Sun Park, Yong Xia, Thi Thinh Nguyen, Young Eun Joo, Kyung Keun Kim, Hark Kyun Kim, and Young Do Jung. 2016. "MicroRNA-375 Functions as a Tumor-Suppressor Gene in Gastric Cancer by Targeting Recepteur d’Origine Nantais" International Journal of Molecular Sciences 17, no. 10: 1633. https://doi.org/10.3390/ijms17101633