Deletion of Phytochelatin Synthase Modulates the Metal Accumulation Pattern of Cadmium Exposed C. elegans
Abstract
:1. Introduction
2. Results and Discussion
2.1. Analysis of Ppcs-1::GFP Expression by Fluorescence Microscopy
2.2. Analysis of Pcs-1 Expression by Quantitative PCR
2.3. Analysis of Metal Accumulation by X-ray Fluorescence Imaging
3. Materials and Methods
3.1. Maintenance and Strains
3.2. Metal Exposures
3.3. Confocal Fluorescence Microscopy
3.4. Quantitative PCR (qPCR)
3.5. X-ray Fluorescence Imaging (XFI)
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Phylum Nematoda (Nematodes) | |||
Class | Order | Species | Accession number |
Chromadorea | Rhabditida | Caenorhabditis elegans | AF299333.1 |
Chromadorea | Rhabditida | Pristionchus pacificus | ABKE02006821 |
Chromadorea | Spirurida | Loa loa | XM_003138991.1 |
Chromadorea | Ascaridida | Ascaris suum | AEUI02000059.1 |
Secernentea | Rhabditida | Caenorhabditis briggsae | FR847113.2 |
Secernentea | Rhabditida | Caenorhabditis remanei | DS268408.1 |
Secernentea | Rhabditida | Caenorhabditis brenneri | XM_003117257.1 |
Secernentea | Rhabditida | Haemonchus contortus | HF966434.1 |
Secernentea | Strongylida | Ancylostoma ceylanicum | KE125293.1 |
Secernentea | Strongylida | Necator americanus | XM_013449818.1 |
Secernentea | Strongylida | Ancylostoma duodenale | KN734493.1 |
Secernentea | Strongylida | Oesophagostomum dentatum | KN567239.1 |
Secernentea | Strongylida | Ancylostoma ceylanicum | KC914882 |
Secernentea | Spirurida | Brugia malayi | XM_001902065.1 |
Secernentea | Ascaridida | Toxocara canis | JPKZ01001678.1 |
Phylum Annelida (Segmented Worms) | |||
Class | Order | Species | Accession number |
Clitellata | Haplotaxida | Lumbricus rubellus | KC981075.1 / KC981074.1 |
Clitellata | Haplotaxida | Eisenia fetida | EF433776.1 |
Clitellata | Haplotaxida | Eisenia andrei | KP770990.1 |
Clitellata | Rhynchobdellida | Helobdella robusta | XM_009013566 |
Polychaeta | Capitellida | Capitella teleta | KB309928 |
Phylum Gastropoda (Molluscs) | |||
Class | Order | Species | Accession number |
Gastropoda | Lottiida | Lottia gigantea | XM_009047767 |
Bivalvia | Ostreoida | Crassostrea gigas | XM_011447075 |
Phylum Platyhelminthes (flatworms) | |||
Class | Order | Species | Accession number |
Trematoda | Strigeidida | Schistosoma mansoni | CABG01000042.1 |
Trematoda | Prosostomata | Schistosoma haematobium | XM_012938931.1 |
Trematoda | Opisthorchiida | Opisthorchis viverrini | XM_009168655 |
Trematoda | Opisthorchiida | Clonorchis sinensis | DF143054 |
Cestoda | Cyclophyllidea | Echinococcus multilocularis | LK031422.1 |
Cestoda | Cyclophyllidea | Echinococcus granulosus | LK028580.1 |
Cestoda | Cyclophyllidea | Hymenolepis microstoma | LK053266.1 |
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Essig, Y.J.; Webb, S.M.; Stürzenbaum, S.R. Deletion of Phytochelatin Synthase Modulates the Metal Accumulation Pattern of Cadmium Exposed C. elegans. Int. J. Mol. Sci. 2016, 17, 257. https://doi.org/10.3390/ijms17020257
Essig YJ, Webb SM, Stürzenbaum SR. Deletion of Phytochelatin Synthase Modulates the Metal Accumulation Pattern of Cadmium Exposed C. elegans. International Journal of Molecular Sciences. 2016; 17(2):257. https://doi.org/10.3390/ijms17020257
Chicago/Turabian StyleEssig, Yona J., Samuel M. Webb, and Stephen R. Stürzenbaum. 2016. "Deletion of Phytochelatin Synthase Modulates the Metal Accumulation Pattern of Cadmium Exposed C. elegans" International Journal of Molecular Sciences 17, no. 2: 257. https://doi.org/10.3390/ijms17020257