Nutritional Signaling Regulates Vitellogenin Synthesis and Egg Development through Juvenile Hormone in Nilaparvata lugens (Stål)
Abstract
:1. Introduction
2. Results
2.1. Identification of Genes Involved in JH Signaling and Phylogenetic Analysis
2.2. Tissue-Specific Expression Profiles of Met and JmtN Examined by qRT-PCR
2.3. AAs Regulate Vg Synthesis in Adult Females
2.4. JmtN Expression Is Regulated by AAs Signaling
2.5. JH Mediates AAs Signaling that Regulates Vg Synthesis
2.6. Silencing Met or JmtN Inhibits Ovarian Development and Reduction in Fecundity
3. Discussion
4. Experimental Section
4.1. Insect Rearing
4.2. Sequence and Phylogenetic Analysis
4.3. Tissue Collection
4.4. RNA Extraction and cDNA Synthesis
4.5. Reverse Transcriptase Quantitative Real-Time PCR (qRT-PCR)
4.6. Western Blot
4.7. Synthesis and Injection of dsRNA
4.8. Ovarian Growth and Fecundity Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Purpose | Primer Name | Primer Sequence (5′–3′) |
---|---|---|
qPCR analysis | Q-met-F | AGTGGCAGCGAGCGATGATT |
Q-met-R | TGAGGCGCAGCAAAAAGGAG | |
Q-jmtN-F | GAACCTGCAGGCCAAACACA | |
Q-jmtN-R | ACCACTCGGTTGGGCTGAAT | |
dsRNA synthesis | met-Fi | GACCCAAGCCACCCTCCAAG |
met-Ri | TCCCCATCGTCAGCCAACTC | |
met-T7Fi | taatacgactcactatagggGACCCAAGCCACCCTCCAAG | |
met-T7Ri | taatacgactcactatagggTCCCCATCGTCAGCCAACTC | |
jmtN-Fi | CTCCAGGCCATTGTCCCTCA | |
jmtN-Ri | TTGGCCTGCAGGTTCTTTGG | |
jmtN-T7Fi | taatacgactcactatagggCTCCAGGCCATTGTCCCTCA | |
jmtN-T7Ri | taatacgactcactatagggTTGGCCTGCAGGTTCTTTGG |
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Lu, K.; Chen, X.; Liu, W.-T.; Zhang, X.-Y.; Chen, M.-X.; Zhou, Q. Nutritional Signaling Regulates Vitellogenin Synthesis and Egg Development through Juvenile Hormone in Nilaparvata lugens (Stål). Int. J. Mol. Sci. 2016, 17, 269. https://doi.org/10.3390/ijms17030269
Lu K, Chen X, Liu W-T, Zhang X-Y, Chen M-X, Zhou Q. Nutritional Signaling Regulates Vitellogenin Synthesis and Egg Development through Juvenile Hormone in Nilaparvata lugens (Stål). International Journal of Molecular Sciences. 2016; 17(3):269. https://doi.org/10.3390/ijms17030269
Chicago/Turabian StyleLu, Kai, Xia Chen, Wen-Ting Liu, Xin-Yu Zhang, Ming-Xiao Chen, and Qiang Zhou. 2016. "Nutritional Signaling Regulates Vitellogenin Synthesis and Egg Development through Juvenile Hormone in Nilaparvata lugens (Stål)" International Journal of Molecular Sciences 17, no. 3: 269. https://doi.org/10.3390/ijms17030269