Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants
Abstract
:1. Introduction
2. Results
2.1. Preparation of TiO2-NTs and Si–TiO2-NT Substrates
2.2. Cell Adhesion and Proliferation
2.3. Osteogenic Gene Expression and Mineralization
2.4. Mechanical Tests
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Fabrication of TiO2 Nanotube-Coated Ti Substrates
4.3. Si Plasma Immersion Ion Implantation (Si-PIII)
4.4. Surface Characterizations
4.5. Cell Culture
4.6. Cell Morphology
4.7. Immunofluorescence of Cytoskeletal Actin
4.8. MTS Assay
4.9. Mineralization Assay
4.10. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.11. Animal Surgeries and Mechanical Tests
4.12. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
Ti | Titanium |
Si | Silicon |
TiO2 | Titanium dioxide |
PIII | Plasma immersion ion implantation |
THA | Total hip arthroplasty |
MSCs | Mesenchymal stem cells |
α-TCP | α-Tri-calcium phosphate |
SEM | Scanning electron microscopy |
XPS | X-ray photoelectron spectroscopy |
α-MEM | α-Mininum essential medium |
SDS | Sodium dodecyl sulphate |
IACUC | Institutional Animal Care and Use Committee |
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Gene | Primers Sequence | Amplicon Size (bp) | Accession Number |
---|---|---|---|
Col-I | Forward: 5′-CCTGAGTCAGCAGATTGAGAACA-3′ | 114 | NM_007742 |
Reverse: 5′-CCAGTACTCTCCGCTCTTCCA-3′ | |||
OC | Forward: 5′-CGCTCTGTCTCTCTGACCTC-3′ | 91 | NM_001037939 |
Reverse: 5′-CACTACCTTATTGCCCTCCTG-3′ | |||
OPN | Forward: 5′-CTTTCACTCCAATCGTCCCTAC-3′ | 99 | NM_001204201 |
Reverse: 5′-CAGAAACCTGGAAACTCCTAGAC-3′ | |||
ALP | Forward: 5′-GGGCATTGTGACTACCACTCG-3′ | 103 | NM_001287172 |
Reverse: 5′-CCTCTGGTGGCATCTCGTTAT-3′ | |||
Runx2 | Forward: 5′-GACACTGCCACCTCTGACTT-3′ | 115 | NM_001145920 |
Reverse: 5′-GATGAAATGCTTGGGAACTG-3′ | |||
GAPDH | Forward: 5′-CATCAAGAAGGTGGTGAAGC-3′ | 198 | NM_001289726 |
Reverse: 5′-CCTGTTGCTGTAGCCGTATT-3′ |
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Zhao, X.; Wang, T.; Qian, S.; Liu, X.; Sun, J.; Li, B. Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants. Int. J. Mol. Sci. 2016, 17, 292. https://doi.org/10.3390/ijms17030292
Zhao X, Wang T, Qian S, Liu X, Sun J, Li B. Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants. International Journal of Molecular Sciences. 2016; 17(3):292. https://doi.org/10.3390/ijms17030292
Chicago/Turabian StyleZhao, Xijiang, Tao Wang, Shi Qian, Xuanyong Liu, Junying Sun, and Bin Li. 2016. "Silicon-Doped Titanium Dioxide Nanotubes Promoted Bone Formation on Titanium Implants" International Journal of Molecular Sciences 17, no. 3: 292. https://doi.org/10.3390/ijms17030292