AfAP2-1, An Age-Dependent Gene of Aechmea fasciata, Responds to Exogenous Ethylene Treatment
Abstract
:1. Introduction
2. Results
2.1. Isolation and Sequence Analysis of AfAP2-1
2.2. Transcript Profiling of AfAP2-1 in A. fasciata
2.3. Relative Expression of AfAP2-1 under the Control of the Circadian Rhythm
2.4. Response to Exogenous Ethylene Treatment
2.5. AfAP2-1 Exhibits Transactivation Activity in Yeast
2.6. Subcellular Localization of AfAP2-1 Protein
2.7. Constitutive Overexpression of AfAP2-1 in Arabidopsis Causes Significantly Delayed Flowering Phenotype
3. Discussion
3.1. Overexpression of AfAP2-1 in Arabidopsis Strongly Delayed Flowering under both LD and SD Conditions
3.2. The Abundance of AfAP2-1 Transcripts Is Negatively Regulated by Exogenous Ethylene Treatment
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Isolation and Sequencing of the AfAP2-1 Gene
4.3. Bioinformatic Analysis
4.4. qRT-PCR Analysis
4.5. Plant Expression Vector Construction and Arabidopsis Transformation
4.6. Transactivation Analysis of AfAP2-1 in Yeast Cells
4.7. Subcellular Localization of AfAP2-1 Protein
4.8. Exogenous Ethylene Treatment of A. fasciata
4.9. Data Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
References
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Lei, M.; Li, Z.-Y.; Wang, J.-B.; Fu, Y.-L.; Ao, M.-F.; Xu, L. AfAP2-1, An Age-Dependent Gene of Aechmea fasciata, Responds to Exogenous Ethylene Treatment. Int. J. Mol. Sci. 2016, 17, 303. https://doi.org/10.3390/ijms17030303
Lei M, Li Z-Y, Wang J-B, Fu Y-L, Ao M-F, Xu L. AfAP2-1, An Age-Dependent Gene of Aechmea fasciata, Responds to Exogenous Ethylene Treatment. International Journal of Molecular Sciences. 2016; 17(3):303. https://doi.org/10.3390/ijms17030303
Chicago/Turabian StyleLei, Ming, Zhi-Ying Li, Jia-Bin Wang, Yun-Liu Fu, Meng-Fei Ao, and Li Xu. 2016. "AfAP2-1, An Age-Dependent Gene of Aechmea fasciata, Responds to Exogenous Ethylene Treatment" International Journal of Molecular Sciences 17, no. 3: 303. https://doi.org/10.3390/ijms17030303