Koumine Attenuates Lipopolysaccaride-Stimulated Inflammation in RAW264.7 Macrophages, Coincidentally Associated with Inhibition of NF-κB, ERK and p38 Pathways
Abstract
:1. Introduction
2. Results
2.1. Koumine Suppresses the Levels of NO and iNOS in LPS-Stimulated RAW264.7 Cells
2.2. Koumine Attenuates the Production of Pro-Inflammatory Cytokines in LPS-Treated RAW264.7 Macrophages
2.3. Koumine Inhibits p38 and ERK Phosphorylation in LPS-Stimulated RAW264.7 Macrophages
2.4. Koumine Hampered IκBα and p65 Phosphorylation in LPS-Stimulated RAW264.7 Macrophages
2.5. Effect of Koumine on DNA-Binding Activity of NF-κB in LPS-Treated RAW264.7 Cells
3. Discussion
4. Experimental Section
4.1. Materials
4.2. Cell Culture
4.3. Assessment of Cell Cytotoxicity
4.4. Measurement of Nitric Oxide and Pro-Inflammatory Cytokines
4.5. Western Blot Analysis
4.6. Nuclear Protein Extracts
4.7. NF-κB DNA-Binding Assay
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contribution
Conflicts of Interest
References
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Yuan, Z.; Matias, F.B.; Wu, J.; Liang, Z.; Sun, Z. Koumine Attenuates Lipopolysaccaride-Stimulated Inflammation in RAW264.7 Macrophages, Coincidentally Associated with Inhibition of NF-κB, ERK and p38 Pathways. Int. J. Mol. Sci. 2016, 17, 430. https://doi.org/10.3390/ijms17030430
Yuan Z, Matias FB, Wu J, Liang Z, Sun Z. Koumine Attenuates Lipopolysaccaride-Stimulated Inflammation in RAW264.7 Macrophages, Coincidentally Associated with Inhibition of NF-κB, ERK and p38 Pathways. International Journal of Molecular Sciences. 2016; 17(3):430. https://doi.org/10.3390/ijms17030430
Chicago/Turabian StyleYuan, Zhihang, Froilan Bernard Matias, Jing Wu, Zengenni Liang, and Zhiliang Sun. 2016. "Koumine Attenuates Lipopolysaccaride-Stimulated Inflammation in RAW264.7 Macrophages, Coincidentally Associated with Inhibition of NF-κB, ERK and p38 Pathways" International Journal of Molecular Sciences 17, no. 3: 430. https://doi.org/10.3390/ijms17030430