Two Novel Relative Double-Stranded RNA Mycoviruses Infecting Fusarium poae Strain SX63
Abstract
:1. Introduction
2. Results and Discussion
2.1. Detection and Complete Genome Sequencing of dsRNA in Fusarium poae Strain SX630
2.2. Both FpV2 and FpV3 Have a Double-Stranded RNA Genome
2.3. Phylogenetic Analysis Based on the RdRp and P1 Sequences
2.4. Identification of “Phytoreo_S7 Domain” in FpV2, FpV3, and Related Unassigned dsRNA Viruses
2.5. Potential Programmed –1 Ribosomal Frameshifting (–1 PRF) in FpV2, FpV3, and Related Unassigned dsRNA Viruses
2.6. A Proposition to Create a New Family Designated Fusagraviridae
3. Materials and Methods
3.1. Fungal Strain and Culture Conditions
3.2. dsRNA Extraction and Purification
3.3. cDNA Synthesis, Molecular Cloning, and Sequencing
3.4. Nucleotide Sequence Analysis
3.5. Phylogenetic Analysis
3.6. GenBank Accession Number
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Virus | Full Sequence | 5′-UTR | 3′-UTR | ORF1 (P1) | ORF2 (RdRP) | |||||
---|---|---|---|---|---|---|---|---|---|---|
Length (bp) | nt % | Length (bp) | nt % | Length (bp) | nt % | nt % | aa % | nt % | aa % | |
FpV2 | 9518 | 42.97 | 1029 | 36.12 | 48 | 44.07 | 39.91 | 24.27 | 44.05 | 29.44 |
FpV3 | 9419 | 1190 | 55 |
Search Target And Virus Name (GenBank Accession No.) a | Size (aa) | % Identity | Overlap (Positions) | Bit Score | E-Value |
---|---|---|---|---|---|
FpV2 (FpV3) | FpV2 (FpV3) | FpV2 (FpV3) | FpV2 (FpV3) | ||
P1 search | |||||
MpRV2 (ALD89096) | 1285 | 34 (38) | 322/960 (340/885) | 482 (558) | 1 × 10−142 (9 × 10–172) |
SsNsV-L (YP_006331064) | 1305 | 33 (49) | 353/1057 (619/1276) | 424 (1125) | 1 × 10–121 (0.0) |
FgV3 (YP_003288788) | 1369 | 32 (44) | 328/1037 (538/1217) | 423 (944) | 8 × 10−121 (0.0) |
GaTV2 (ADO60932) | 1313 | 33 (47) | 353/1076 (635/1351) | 397 (1123) | 2 × 10−112 (0.0) |
BcRV1 (YP_009115497) | 1314 | 32 (47) | 347/1075 (644/1363) | 395 (1118) | 1 × 10−111 (0.0) |
FvV2 (AEZ54145) | 1347 | 27 (28) | 295/1083 (257/910) | 337 (329) | 6 × 10−92 (1 × 10−89) |
FvV1 (AEZ54147) | 1311 | 26 (29) | 281/1066 (286/983) | 335 (357) | 1 × 10−91 (5 × 10−99) |
PgV2 (CAJ34334) b | >1696 | 22 (28) | 195/886 (117/424) | 123 (124) | 9 × 10−25 (4 × 10−25) |
PmeV (YP_009179229) | 1596 | 25 (ND) | 93/365 (ND) | 91.7 (ND) | 5 × 10−15 (ND) |
RdRp search | |||||
MpRV2 (ALD89097) | 1082 | 36 (37) | 386/1076 (410/1116) | 625 (664) | 0.0 (0.0) |
FgV3 (YP_003288789) | 1311 | 32 (44) | 421/1324 (605/1366) | 568 (1041) | 1 × 10−174 (0.0) |
FvV1 (AEZ54148) | 1289 | 30 (31) | 401/1328 (408/1305) | 556 (561) | 2 × 10−170 (3 × 10−172) |
BcRV1 (YP_009115498) | 1338 | 31 (43) | 427/1356 (594/1372) | 556 (993) | 5 × 10−170 (0.0) |
SsNsV-L (CEZ26308) | 1338 | 31 (44) | 421/1338 (601/1370) | 556 (1056) | 7 × 10−170 (0.0) |
FvV2 (AEZ54146) | 1310 | 30 (31) | 406/1346 (662/1398) | 535 (558) | 1 × 10−162 (6 × 10−171) |
DsRV1 (YP_003359178) | 1110 | 28 (32) | 305/1094 (322/1005) | 365 (436) | 8 × 10−103 (5 × 10−128) |
PgV2 (CAJ34335) | 1153 | 36 (38) | 226/632 (234/623) | 331 (380) | 5 × 10−91 (2 × 10−107) |
PiRV3 (AEX87902) | 1011 | 29 (31) | 190/645 (188/614) | 243 (241) | 1 × 10−62 (8 × 10−62) |
PmeV (YP_009179230) | 1156 | 31 (33) | 166/535 (197/604) | 241 (280) | 2 × 10−61 (3 × 10−74) |
GaTV2 (ADO60933) b | >613 | 31(44) | 196/632 (274/626) | 230 (456) | 3 × 10−60 (6 × 10−141) |
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Wang, L.; Zhang, J.; Zhang, H.; Qiu, D.; Guo, L. Two Novel Relative Double-Stranded RNA Mycoviruses Infecting Fusarium poae Strain SX63. Int. J. Mol. Sci. 2016, 17, 641. https://doi.org/10.3390/ijms17050641
Wang L, Zhang J, Zhang H, Qiu D, Guo L. Two Novel Relative Double-Stranded RNA Mycoviruses Infecting Fusarium poae Strain SX63. International Journal of Molecular Sciences. 2016; 17(5):641. https://doi.org/10.3390/ijms17050641
Chicago/Turabian StyleWang, Luan, Jingze Zhang, Hailong Zhang, Dewen Qiu, and Lihua Guo. 2016. "Two Novel Relative Double-Stranded RNA Mycoviruses Infecting Fusarium poae Strain SX63" International Journal of Molecular Sciences 17, no. 5: 641. https://doi.org/10.3390/ijms17050641