miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model
Abstract
:1. Introduction
2. Results
2.1. Expression of miR-126 in Laser-Induced Choroidal Neovascularization (CNV) Mouse Model
2.2. Changes of miR-126 Target Genes in the Eyes of CNV Mice
2.3. Restoration of miR-126 Levels Reversed High mRNA and Protein Expression of Target Genes in Human Microvascular Endothelial Cell (HMEC)
2.4. Transfection of miR-126 Decreased Tube Formation in HMECs
2.5. Effect of miR-126 Overexpression on VEGF-Induced Migration of HMEC
2.6. Restoration of miR-126 in Laser-Induced CNV Lesions in Mice Eyes
3. Discussion
4. Experimental Section
4.1. Animals
4.1.1. Establishment of Laser-Induced CNV Mouse Model
4.1.2. Micron III Imaging
4.1.3. Choroidal Flatmount
4.1.4. Intravitreal Injection of miRNA
4.2. Cells
4.2.1. In Vitro Transfection of HMECs
4.2.2. Tube Formation Assay and Scratch Wound Migration Assay
4.3. RNA Preparation and qPCR
4.4. Western Blotting Analysis
4.5. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wang, L.; Lee, A.Y.W.; Wigg, J.P.; Peshavariya, H.; Liu, P.; Zhang, H. miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model. Int. J. Mol. Sci. 2016, 17, 895. https://doi.org/10.3390/ijms17060895
Wang L, Lee AYW, Wigg JP, Peshavariya H, Liu P, Zhang H. miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model. International Journal of Molecular Sciences. 2016; 17(6):895. https://doi.org/10.3390/ijms17060895
Chicago/Turabian StyleWang, Lei, Amy Yi Wei Lee, Jonathan P. Wigg, Hitesh Peshavariya, Ping Liu, and Hong Zhang. 2016. "miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model" International Journal of Molecular Sciences 17, no. 6: 895. https://doi.org/10.3390/ijms17060895