The Effects of Magnesium Ions on the Enzymatic Synthesis of Ligand-Bearing Artificial DNA by Template-Independent Polymerase
Abstract
:1. Introduction
2. Results and Discussion
2.1. Interaction of MgII Ions with Hydroxypyridone-Bearing Nucleotides (H)
2.2. Effects of MgII Ions on the Terminal Deoxynucleotidyl Transferase (TdT)-Catalyzed Polymerization of dHTP
2.3. Effects of MgII Ions on the Affinity between TdT and H-Tailed Strands
3. Materials and Methods
3.1. Materials and General Procedures
3.2. Ultraviolet-Visible (UV) Spectroscopy
3.3. Primer Extension Experiments
3.4. Time-Course Analysis
3.5. Electrophoresis Mobility Shift Assay (EMSA)
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
H | hydroxypydirone-bearing nucleoside |
dHTP | hydroxypydirone-bearing nucleoside triphosphate |
TdT | terminal deoxynucleotidyl transferase |
PAGE | polyacrylamide gel electrophoresis |
EMSA | electrophoresis mobility shift assay |
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Takezawa, Y.; Kobayashi, T.; Shionoya, M. The Effects of Magnesium Ions on the Enzymatic Synthesis of Ligand-Bearing Artificial DNA by Template-Independent Polymerase. Int. J. Mol. Sci. 2016, 17, 906. https://doi.org/10.3390/ijms17060906
Takezawa Y, Kobayashi T, Shionoya M. The Effects of Magnesium Ions on the Enzymatic Synthesis of Ligand-Bearing Artificial DNA by Template-Independent Polymerase. International Journal of Molecular Sciences. 2016; 17(6):906. https://doi.org/10.3390/ijms17060906
Chicago/Turabian StyleTakezawa, Yusuke, Teruki Kobayashi, and Mitsuhiko Shionoya. 2016. "The Effects of Magnesium Ions on the Enzymatic Synthesis of Ligand-Bearing Artificial DNA by Template-Independent Polymerase" International Journal of Molecular Sciences 17, no. 6: 906. https://doi.org/10.3390/ijms17060906