SMA Human iPSC-Derived Motor Neurons Show Perturbed Differentiation and Reduced miR-335-5p Expression
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Cell Culture and Reprogramming to hiPSCs
4.3. Cytofluorimetry
4.4. Expression Analyses
4.5. hiPSCs Differentiation and Immunocytochemical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Primers | Forward (5′-3′) | Reverse (5′-3′) |
---|---|---|
5S | TCGTCTGATCTCGGAAGCTAAGCA | AAAGCCTACAGCACCCGGTATT |
OCT4 | AACCTGGAGTTTGTGCCAGGGTTT | TGAACTTCACCTTCCCTCCAACCA |
SOX2 | AGAAGAGGAGAGAGAAAGAAAGGGAGAGA | GAGAGAGGCAAACTGGAATCAGGATCAAA |
NANOG | CCTGAAGACGTGTGAAGATGAG | GCTGATTAGGCTCCAACCATAC |
NCAM | ATGGAAACTCTATTAAAGTGAACCTG | TAGACCTCATACTCAGCATTCCAGT |
Isl1 | GAATGGCATGCGGCATGTTTGA | CGCATTTGATCCCGTACAACCTGA |
Lhx3 | TCGGACAAGGACAGCGTTCAG | TTTCCGCCAAGGAAGGCTCATCG |
HB9 | CACCGAGACCCAGGTGAAGATTT | CCCTTCTGTTTCTCCGCTTCCT |
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Murdocca, M.; Ciafrè, S.A.; Spitalieri, P.; Talarico, R.V.; Sanchez, M.; Novelli, G.; Sangiuolo, F. SMA Human iPSC-Derived Motor Neurons Show Perturbed Differentiation and Reduced miR-335-5p Expression. Int. J. Mol. Sci. 2016, 17, 1231. https://doi.org/10.3390/ijms17081231
Murdocca M, Ciafrè SA, Spitalieri P, Talarico RV, Sanchez M, Novelli G, Sangiuolo F. SMA Human iPSC-Derived Motor Neurons Show Perturbed Differentiation and Reduced miR-335-5p Expression. International Journal of Molecular Sciences. 2016; 17(8):1231. https://doi.org/10.3390/ijms17081231
Chicago/Turabian StyleMurdocca, Michela, Silvia Anna Ciafrè, Paola Spitalieri, Rosa Valentina Talarico, Massimo Sanchez, Giuseppe Novelli, and Federica Sangiuolo. 2016. "SMA Human iPSC-Derived Motor Neurons Show Perturbed Differentiation and Reduced miR-335-5p Expression" International Journal of Molecular Sciences 17, no. 8: 1231. https://doi.org/10.3390/ijms17081231