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Article

Characterisation and Antioxidant Activity of Crude Extract and Polyphenolic Rich Fractions from C. incanus Leaves

by
Antonella Gori
1,2,
Francesco Ferrini
1,
Maria Cristina Marzano
1,
Massimiliano Tattini
3,
Mauro Centritto
2,
Maria Camilla Baratto
4,
Rebecca Pogni
4 and
Cecilia Brunetti
1,2,*
1
Department of Agrifood Production and Environmental Sciences (DiSPAA), University of Florence, 50019 Sesto Fiorentino (Florence), Italy
2
Trees and Timber Institute (IVALSA), The National Research Council of Italy (CNR), 50019 Sesto Fiorentino (Florence), Italy
3
Institute for Plant Protection (IPSP), The National Research Council of Italy (CNR), 50019 Sesto Fiorentino (Florence), Italy
4
Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2016, 17(8), 1344; https://doi.org/10.3390/ijms17081344
Submission received: 29 June 2016 / Revised: 5 August 2016 / Accepted: 10 August 2016 / Published: 17 August 2016
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

Cistus incanus (Cistaceae) is a Mediterranean evergreen shrub. Cistus incanus herbal teas have been used as a general remedy in traditional medicine since ancient times. Recent studies on the antioxidant properties of its aqueous extracts have indicated polyphenols to be the most active compounds. However, a whole chemical characterisation of polyphenolic compounds in leaves of Cistus incanus (C. incanus) is still lacking. Moreover, limited data is available on the contribution of different polyphenolic compounds towards the total antioxidant capacity of its extracts. The purpose of this study was to characterise the major polyphenolic compounds present in a crude ethanolic leaf extract (CEE) of C. incanus and develop a method for their fractionation. Superoxide anion, hydroxyl and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assays were also performed to evaluate the antioxidant properties of the obtained fractions. Three different polyphenolic enriched extracts, namely EAC (Ethyl Acetate Fraction), AF1 and AF2 (Aqueos Fractions), were obtained from CEE. Our results indicated that the EAC, enriched in flavonols, exhibited a higher antiradical activity compared to the tannin enriched fractions (AF1 and AF2). These findings provide new perspectives for the use of the EAC as a source of antioxidant compounds with potential uses in pharmaceutical preparations.
Keywords: polyphenolic enriched fractions; flavonols; LC–MS/MS (liquid chromatography–tandem Mass Spectrometry); DPPH radical-scavenging activity polyphenolic enriched fractions; flavonols; LC–MS/MS (liquid chromatography–tandem Mass Spectrometry); DPPH radical-scavenging activity
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MDPI and ACS Style

Gori, A.; Ferrini, F.; Marzano, M.C.; Tattini, M.; Centritto, M.; Baratto, M.C.; Pogni, R.; Brunetti, C. Characterisation and Antioxidant Activity of Crude Extract and Polyphenolic Rich Fractions from C. incanus Leaves. Int. J. Mol. Sci. 2016, 17, 1344. https://doi.org/10.3390/ijms17081344

AMA Style

Gori A, Ferrini F, Marzano MC, Tattini M, Centritto M, Baratto MC, Pogni R, Brunetti C. Characterisation and Antioxidant Activity of Crude Extract and Polyphenolic Rich Fractions from C. incanus Leaves. International Journal of Molecular Sciences. 2016; 17(8):1344. https://doi.org/10.3390/ijms17081344

Chicago/Turabian Style

Gori, Antonella, Francesco Ferrini, Maria Cristina Marzano, Massimiliano Tattini, Mauro Centritto, Maria Camilla Baratto, Rebecca Pogni, and Cecilia Brunetti. 2016. "Characterisation and Antioxidant Activity of Crude Extract and Polyphenolic Rich Fractions from C. incanus Leaves" International Journal of Molecular Sciences 17, no. 8: 1344. https://doi.org/10.3390/ijms17081344

APA Style

Gori, A., Ferrini, F., Marzano, M. C., Tattini, M., Centritto, M., Baratto, M. C., Pogni, R., & Brunetti, C. (2016). Characterisation and Antioxidant Activity of Crude Extract and Polyphenolic Rich Fractions from C. incanus Leaves. International Journal of Molecular Sciences, 17(8), 1344. https://doi.org/10.3390/ijms17081344

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