Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers’ Pneumoconiosis: A Case-Control Study
Abstract
:1. Introduction
2. Results
2.1. LncRNA-ATB Expression in the Plasma of All Participants
2.2. Association between LncRNA-ATB Expression and Coal Workers’ Pneumoconiosis (CWP)
2.3. Relationship between LncRNA-ATB Expression and Clinical/Biological Features in CWP Patients
2.4. Plasma LncRNA-ATB Expression Can Be a Potential CWP Biomarker
3. Discussion
4. Materials and Methods
4.1. Study Population
4.2. Total RNA Extraction and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.3. Enzyme-Linked Immunosorbent Assay for Plasma Measurements (ELISA)
4.4. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Variables | Normal Controls (n = 168) | Healthy Coal Miners (n = 72) | CWP Patients (n = 137) | p-Value |
---|---|---|---|---|
Age (years, mean ± SD) | 58.83 ± 10.63 | 57.69 ± 10.86 | 59.27 ± 7.88 | 0.65 a |
Age (n, %) | ||||
<55 | 45 (26.79) | 54 (75.00) | 40 (29.19) | <0.05 b |
≥55 | 123 (73.21) | 18 (25.00) | 97 (70.81) | |
BMI (kg/m2, mean ± SD) | 24.61 ± 3.33 | 25.50 ± 2.88 | 22.36 ± 3.46 #,* | <0.05 a |
Net year in dust (n, %) | ||||
≤30 | / | 62 (86.11) | 90 (65.69) | <0.05 b |
>30 | / | 10 (13.89) | 47 (34.31) | |
Smoking status (n, %) | ||||
Non-smoking | 74 (44.05) | 27 (37.50) | 40 (29.20) | <0.05 b |
Smoking | 94 (55.95) | 45 (62.50) | 97 (70.80) | |
Blood pressure systolic (mm Hg, mean ± SD) | 127.86 ± 21.51 | n.d. | 140.20 ± 23.76 | <0.05 d |
Blood pressure diastolic (mm Hg, mean ± SD) | 82.85 ± 16.67 | n.d. | 76.20 ± 13.06 | <0.05 d |
FVC (% PRED FVC) | 90.65 (64.90–120.30) | 93.55 (71.40–109.40) | 66.20 (28.20–104.50) #,* | <0.05 c |
FEV1 (% PRED FEV1) | 97.25 (67.10–95.44) | 98.65 (89.00–111.20) | 62.00 (17.70–108.40) #,* | <0.05 c |
FEV1/FVC (% PRED) | 80.75 (68.10–86.83) | 83.94 (63.11–97.15) | 74.81 (43.83–98.64) #,* | <0.05 c |
TGF-β1 (pg/mL) | 303.41 ± 28.38 | 425.64 ± 33.98 # | 569.99 ± 64.13 #,* | <0.05 a |
Col-3 (ng/mL) | 67.28 ± 7.79 | 70.26 ± 8.17 | 112.15 ± 9.16 #,* | <0.05 a |
MMP2 (ng/mL) | 142.27 (114.16–172.00) | 166.73 (157.68–183.63) # | 206.32 (186.32–219.16) #,* | <0.05 c |
MMP9 (ng/mL) | 55.84 (45.03–68.81) | 98.88 (75.03–112.32) # | 123.68 (105.16–143.27) #,* | <0.05 c |
Col-1 (ng/mL) | 28.65 (26.53–29.53) | 29.90 (27.28–32.25) | 34.15 (31.90.90–36.53) #,* | <0.05 c |
Variables | LncRNA-ATB Expression Levels (Fold Change) | Per 1 Log-Unit Increment | p-Value * | ||
---|---|---|---|---|---|
First Group <0.7559 | Second Group 0.7559–1.1433 | Third Group >1.1433 | |||
Healthy controls vs. CWP | |||||
No. of cases/control subjects | 26/55 | 39/55 | 72/55 | ||
Model 1 (OR: 95% CI) | 1.00 (referent) | 1.47 (0.79–2.74) | 2.67 (1.49–4.78) | 2.75 (1.69–4.48) | <0.05 |
Model 2 (OR: 95% CI) | 1.00 (referent) | 1.48 (0.80–2.75) | 2.68 (1.50–4.80) | 2.76 (1.70–4.49) | <0.05 |
Model 3 (OR: 95% CI) | 1.00 (referent) | 1.43 (0.75–2.74) | 2.34 (1.28–4.30) | 2.56 (1.54–4.27) | <0.05 |
Model 4 (OR: 95% CI) | 1.00 (referent) | 1.41 (0.73–2.72) | 2.39 (1.29–4.42) | 2.57 (1.52–4.33) | <0.05 |
Healthy coal miners vs. CWP | <0.7542 | 0.7542–1.2161 | >1.2161 | ||
No. of cases/control subjects | 49/23 | 75/24 | 85/25 | ||
Model 1 (OR: 95% CI) | 1.00 (referent) | 1.77 (0.85–3.70) | 2.25 (1.08–4.68) | 1.82 (1.05–3.17) | <0.05 |
Model 2 (OR: 95% CI) | 1.00 (referent) | 1.86 (0.84–4.15) | 2.25 (1.02–4.97) | 1.83 (1.01–3.35) | <0.05 |
Model 3 (OR: 95% CI) | 1.00 (referent) | 1.54 (0.64–3.70) | 1.78 (0.74–4.31) | 1.90 (0.97–3.72) | 0.06 |
Model 4 (OR: 95% CI) | 1.00 (referent) | 1.47 (0.59–3.65) | 2.05 (0.81–5.19) | 2.25 (1.07–4.71) | <0.05 |
Model 5 (OR: 95% CI) | 1.00 (referent) | 1.65 (0.65–4.18) | 1.90 (0.74–4.89) | 2.17 (1.04–4.53) | <0.05 |
Variables | LncRNA-ATB | TGF-β1 | MMP-2 | MMP-9 | Col-1 | Col-3 | % PRED VC | % PRED FVC | % PRED FEV1 | FEV1/FVC |
---|---|---|---|---|---|---|---|---|---|---|
LncRNA-ATB | 1.00 | 0.30 * | 0.07 | −0.03 | 0.09 | 0.02 | −0.18 * | −0.18 * | −0.10 | 0.05 |
TGF-β1 | 1.00 | −0.04 | 0.09 | 0.18 | −0.06 | 0.47 * | −0.55 * | −0.50 * | −0.30 * | |
MMP-2 | 1.00 | 0.04 | 0.09 | −0.03 | −0.02 | −0.04 | −0.08 | −0.06 | ||
MMP-9 | 1.00 | 0.29 | 0.07 | 0.23 * | −0.20 | −0.29 * | −0.31 * | |||
Col-1 | 1.00 | 0.14 | 0.28 * | −0.25 * | −0.31 * | −0.20 | ||||
Col-3 | 1.00 | −0.15 | −0.08 | −0.13 | −0.07 | |||||
% PRED VC | 1.00 | 0.93 * | 0.88 * | 0.47 * | ||||||
% PRED FVC | 1.00 | 0.90* | 0.42 * | |||||||
% PRED FEV1 | 1.00 | 0.75 * | ||||||||
FEV1/FVC | 1.00 |
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Ma, J.; Cui, X.; Rong, Y.; Zhou, Y.; Guo, Y.; Zhou, M.; Xiao, L.; Chen, W. Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers’ Pneumoconiosis: A Case-Control Study. Int. J. Mol. Sci. 2016, 17, 1367. https://doi.org/10.3390/ijms17081367
Ma J, Cui X, Rong Y, Zhou Y, Guo Y, Zhou M, Xiao L, Chen W. Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers’ Pneumoconiosis: A Case-Control Study. International Journal of Molecular Sciences. 2016; 17(8):1367. https://doi.org/10.3390/ijms17081367
Chicago/Turabian StyleMa, Jixuan, Xiuqing Cui, Yi Rong, Yun Zhou, Yanjun Guo, Min Zhou, Lili Xiao, and Weihong Chen. 2016. "Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers’ Pneumoconiosis: A Case-Control Study" International Journal of Molecular Sciences 17, no. 8: 1367. https://doi.org/10.3390/ijms17081367