Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase
Abstract
:1. Introduction
2. Results
2.1. Viscosity
2.2. Reducing Sugar-Ends Content
2.3. DPPH (2,2-Diphenyl-1-picrylhydrazyl)
2.4. Ferric Reducing Antioxidant Power (FRAP)
2.5. Antimicrobial Activity
3. Materials and Methods
3.1. Materials
3.2. Hydrosols Preparation
3.3. Viscosity Determination
3.4. Quantitive Determination of Reducing Sugar-Ends
3.5. Free Radical Scavenging Activity
3.6. Ferric Reducing Ion Antioxidant Power
3.7. Antimicrobial Activity
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Variants Coding | Scavenging Effect of DPPH (%) | FRAP (µM Fe(II)/mL) |
---|---|---|
C0T0 | 7.6 ± 0.73 a,b | 61.6 ± 7.08 a |
C0.05T0 | 9.2 ± 0.57 a,b | 71.6 ± 4.74 a,b |
C0.1T0 | 11.5 ± 2.16 b | 87.9 ± 6.16 b,c |
C0T3 | 6.8 ± 1.57 a | 81.6 ± 2.97 a,b,c |
C0.05T3 | 39.5 ± 2.13 c | 161.3 ± 7.70 d |
C0.1T3 | 45.2 ± 0.14 d | 266.2 ± 13.29 e |
C0T20 | 5.9 ± 0.49 a | 93.6 ± 3.76 c |
C0.05T20 | 47.1 ± 2.46 d | 275.6 ± 15.93 e |
C0.1T20 | 52.1 ± 5.50 e | 329.4 ± 25.07 f |
Variants Coding | Log Reduction | |||
---|---|---|---|---|
P. fluorescens | Y. enterocolitica | B. cereus | S. aureus | |
C0T0 | 2.2 ± 0.26 | 0.3 ± 0.13 | 9.2 ± 0.06 | 0.9 ± 0.13 |
C0.05T0 | 9.5 ± 0.05 | 0.3 ± 0.06 | 9.2 ± 0.06 | 2.0 ± 0.23 |
C0.1T0 | 9.5 ± 0.05 | 0.5 ± 0.07 | 9.2 ± 0.06 | 2.2 ± 0.06 |
C0T3 | 4.7 ± 4.12 | 0.3 ± 0.11 | 9.2 ± 0.06 | 0.9 ± 0.27 |
C0.05T3 | 9.5 ± 0.05 | 1.5 ± 0.32 | 9.2 ± 0.06 | 1.7 ± 0.48 |
C0.1T3 | 9.5 ± 0.05 | 9.3 ± 0.07 | 9.2 ± 0.06 | 9.2 ± 0.06 |
C0T20 | 2.3 ± 0.23 | 0.7 ± 0.26 | 9.2 ± 0.06 | 1.4 ± 0.10 |
C0.05T20 | 9.5 ± 0.05 | 9.3 ± 0.07 | 9.2 ± 0.06 | 9.2 ± 0.06 |
C0.1T20 | 9.5 ± 0.05 | 9.3 ± 0.07 | 9.2 ± 0.06 | 9.2 ± 0.06 |
Variants Coding | Variation Factors | Constant Factors | ||||
---|---|---|---|---|---|---|
Cellulase (%) | Time (h) | Chitosan (%) | HPMC (%) | Glycerol (%) | Lactic Acid (%) | |
C0T0 | 0 | 0 | 1 | 1 | 25 | 0.25 |
C0.05T0 | 0.05 | |||||
C0.1T0 | 0.1 | |||||
C0T3 | 0 | 3 | ||||
C0.05T3 | 0.05 | |||||
C0.1T3 | 0.1 | |||||
C0T20 | 0 | 20 | ||||
C0.05T20 | 0.05 | |||||
C0.1T20 | 0.1 |
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Zimoch-Korzycka, A.; Bobak, Ł.; Jarmoluk, A. Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase. Int. J. Mol. Sci. 2016, 17, 1436. https://doi.org/10.3390/ijms17091436
Zimoch-Korzycka A, Bobak Ł, Jarmoluk A. Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase. International Journal of Molecular Sciences. 2016; 17(9):1436. https://doi.org/10.3390/ijms17091436
Chicago/Turabian StyleZimoch-Korzycka, Anna, Łukasz Bobak, and Andrzej Jarmoluk. 2016. "Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase" International Journal of Molecular Sciences 17, no. 9: 1436. https://doi.org/10.3390/ijms17091436