De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control
Abstract
:1. Introduction
2. Results
2.1. Assembly
2.2. GO Annotation
2.3. Comparative Analysis of the Transcriptome of M. enterolobii
2.3.1. CAZymes
2.3.2. Kinases
2.3.3. Neuropeptides
2.3.4. RNAi Pathway
2.3.5. Immune Signaling
2.3.6. Secretory Proteins
2.4. Expression of the Candidate Genes Suppressed BAX-Induced Cell Death in N. benthamiana
3. Discussion
4. Materials and Methods
4.1. Sample Treatment
4.2. RNA Extraction, cDNA Synthesis and Sequencing
4.3. Assembly and GO Annotation
4.4. Phylogenetic and Comparative Analysis of Orthologous Families
4.5. Comparative Analysis of the Transcriptome
4.6. Functional Verification of Three Putative Effectors
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Assembly Metric | Library | Contigs | Singletons |
---|---|---|---|
Total base pairs | 165,040,879 | – | – |
Total reads | 408,663 | – | – |
Reads in assembly | 355,760 | – | – |
Average length (bp) | 403 | 1202 | 380 |
EST number | – | 8193 | 31,860 |
Length range (bp) | – | 62–18,104 | 50–1043 |
Operon number | – | 8143 | 29,043 |
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Li, X.; Yang, D.; Niu, J.; Zhao, J.; Jian, H. De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control. Int. J. Mol. Sci. 2016, 17, 1442. https://doi.org/10.3390/ijms17091442
Li X, Yang D, Niu J, Zhao J, Jian H. De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control. International Journal of Molecular Sciences. 2016; 17(9):1442. https://doi.org/10.3390/ijms17091442
Chicago/Turabian StyleLi, Xiangyang, Dan Yang, Junhai Niu, Jianlong Zhao, and Heng Jian. 2016. "De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control" International Journal of Molecular Sciences 17, no. 9: 1442. https://doi.org/10.3390/ijms17091442