Characteristics of Three Thioredoxin Genes and Their Role in Chilling Tolerance of Harvested Banana Fruit
Abstract
:1. Introduction
2. Results
2.1. Cloning of MaTrx6, MaTrx9 and MaTrx12 Genes from Banana Fruit
2.2. Sequence and Phylogenetic Analysis of Banana Fruit Trx Genes
2.3. Subcellular Localization of MaTrx6, MaTrx9 and MaTrx12
2.4. Effect of Ethylene and 1-MCP Pretreatments on the Development of Chilling Injury in Harvested Banana Fruit
2.5. Expression of MaTrx6, MaTrx9 and MaTrx12 Genes in Ethylene- and 1-MCP-Treated Banana Fruit in Response to Low Temperature Stress
2.6. Effect of MaTrx6, MaTrx9 and MaTrx12 Heterologous Expression on the Growth of a Cytoplasmic Trx-Deficient Yeast Strain under Hydrogen Peroxide Treatment
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Measurement of Chilling Injury (CI) Index
4.3. Color Measurement
4.4. RNA Isolation and cDNA Synthesis
4.5. Amplification of MaTrx Full Length cDNAs
4.6. Sequence and Phylogenetic Analysis
4.7. Real-Time Quantitative PCR (RT-qPCR) Analysis
4.8. Subcellular Localization of MaTrxs
4.9. Heterologous Complementation Analysis
4.10. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
1-MCP | 1-Methylcyclopropene |
CaMV | Cauliflower Mosaic Virus |
CI | Chilling Injury |
GFP | Green Fluorescent Protein |
H2O2 | Hydrogen peroxide |
iTRAQ | Isobaric Tags for Relative and Absolute Quantitation |
ORF | Open Reading Frame |
PEG | Polyethylene Glycol |
ROS | Reactive Oxygen Species |
Trx | Thioredoxin |
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Name | Nucleotide Sequence (5’-3’) |
---|---|
Trx-For1 | CGACTTCGCCGCCACNTGGTGYGGNC |
Trx-For2 | CGTGGAGTTCGTCAAGATCGAYGTNGAYGA |
MaTrx6-5RACE1 | CGGAGTACTGGGCGGCCATC |
MaTrx6-5RACE2 | CTCCACGAAGCGGCACGGC |
MaTrx9-5RACE1 | TGTTCCTCGTGGTTTAGTTG |
MaTrx9-5RACE2 | ATCCTCAGCCGACTTGATA |
MaTrx12-5RACE1 | GTTGCTGAGATGTCCCACGA |
MaTrx12-5RACE2 | GCCACAACTATCCTGCCATC |
eGFP-MaTrx6-Sal I-F | AGTCGACATGGGTGGTTTCTTCTCCAGCC |
eGFP-MaTrx6-Sal I-R | AGTCGACGGTGTTGATCTGCTCTTGGATCC |
eGFP-MaTrx9-Mlu I-F | CGACGCGTATGGCGGATGCTTTGGCTC |
eGFP-MaTrx9-Mlu I-R | CGACGCGTTCTACCGGCTGCGGC |
eGFP-MaTrx12-Sal I-F | AGTCGACATGGGAAACTGCATAAGAAAGAATGTGAG |
eGFP-MaTrx12-Sal I-R | AGTCGACTCGTAGCGGGAGAGTAGCATC |
MaTrx6-F | AGGAGGTGGGCAGAATCGT |
MaTrx6-R | TGGTGGCGGTAATACAGACAG |
MaTrx9-F | TCACCTACACAAGAACGCCT |
MaTrx9-R | CCACATACATAACCAATAAGCAG |
MaTrx12-F | TCGTGGGACATCTCAGCAAC |
MaTrx12-R | CTTTCTTCATCGTAGCGGGA |
Actin-F | TGGTATGGAAGCCGCTGGTA |
Actin-R | TCTGCTGGAATGTGCTGAGG |
pFL61-MaTrx6-Not I-F | TTGCGGCCGCATGGGTGGTTTCTTCTCCAGCC |
pFL61-MaTrx6-Not I-R | TTGCGGCCGCTTAGGTGTTGATCTGCTCCTGGATCC |
pFL61-MaTrx9-Not I-F | TTGCGGCCGCATGGCGGATGCTTTGGCTC |
pFL61-MaTrx9-Not I-R | TTGCGGCCGCTCATCTACCGGCTGCGGCAAAAG |
pFL61-MaTrx12-Not I-F | TTGCGGCCGCATGGGAAACTGCATAAGAAAG |
pFL61-MaTrx12-Not I-R | TTGCGGCCGCTCATCGTAGCGGGAGAGTAGC |
FLDR | CTATTATTTTAGCGTAAAGGATGG |
FLGA | CTCTTTTTTACAGATCATCAAGG |
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Wu, F.; Li, Q.; Yan, H.; Zhang, D.; Jiang, G.; Jiang, Y.; Duan, X. Characteristics of Three Thioredoxin Genes and Their Role in Chilling Tolerance of Harvested Banana Fruit. Int. J. Mol. Sci. 2016, 17, 1526. https://doi.org/10.3390/ijms17091526
Wu F, Li Q, Yan H, Zhang D, Jiang G, Jiang Y, Duan X. Characteristics of Three Thioredoxin Genes and Their Role in Chilling Tolerance of Harvested Banana Fruit. International Journal of Molecular Sciences. 2016; 17(9):1526. https://doi.org/10.3390/ijms17091526
Chicago/Turabian StyleWu, Fuwang, Qing Li, Huiling Yan, Dandan Zhang, Guoxiang Jiang, Yueming Jiang, and Xuewu Duan. 2016. "Characteristics of Three Thioredoxin Genes and Their Role in Chilling Tolerance of Harvested Banana Fruit" International Journal of Molecular Sciences 17, no. 9: 1526. https://doi.org/10.3390/ijms17091526