Acquisition of Flavescence Dorée Phytoplasma by Scaphoideus titanus Ball from Different Grapevine Varieties
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Vector and Flavescence Dorée Phytoplasma Source Plants
4.2. Flavescence Dorée Phytoplasma Acquisition Experiments
4.3. DNA Extraction and Phytoplasma Detection by PCR
4.4. Flavescence Dorée Phytoplasma Quantification by qPCR
4.5. Data Analyses
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Variety | Number of Plants Used for FDP Quantification | FDP Load * | Number of Plants Used for Acquisition | PCR Positive/Total Tested Insects |
---|---|---|---|---|
Arneis | 12 | 230.50 ± 55.88 a | 3 | 45/132 |
Brachetto | 9 | 1136.19 ± 177.37 b | 2 | 16/41 |
Dolcetto | 7 | 677.17 ± 171.54 b | 2 | 30/62 |
Freisa | 3 | 1090.00 ± 346.58 b | 3 | 37/89 |
Moscato | 12 | 1216.81 ± 386.59 b | 5 | 16/178 |
Timorasso | 4 | 1294.70 ± 378.72 b | 4 | 22/101 |
Source | Type III | ||
---|---|---|---|
Wald Chi-Square | df | Sign. | |
(Intercept) | 27.892 | 1 | 0.000 |
Variety | 26.796 | 5 | 0.000 |
FDP | 13.788 | 1 | 0.000 |
Variety× FDP | 19.628 | 5 | 0.001 |
Parameter | Parameter Estimation | 95% Wald Confidence Interval | Significance | |
---|---|---|---|---|
Upper | Lower | |||
Intercept | 0.432 | −0.544 | 1.408 | 0.385 |
Arneis | −3.590 | −5.377 | −1.802 | 0.000 |
Brachetto | −2.801 | −6.339 | 0.738 | 0.121 |
Freisa | −2.023 | −3.565 | −0.482 | 0.010 |
Moscato | −2.457 | −3.992 | −0.923 | 0.002 |
Timorasso | −3.538 | −5.148 | −1.929 | 0.000 |
Dolcetto | 0 a | – | – | – |
FDP | −0.001 | −0.003 | 0.001 | 0.248 |
ARNEIS × FDP | 0.023 | 0.010 | 0.035 | 0.000 |
BRACHETTO × FDP | 0.003 | −0.001 | 0.007 | 0.109 |
FREISA × FDP | 0.002 | 6.342 × 10−5 | 0.005 | 0.044 |
MOSCATO × FDP | 0.001 | −0.002 | 0.003 | 0.554 |
TIMORASSO × FDP | 0.002 | 0.000 | 0.005 | 0.024 |
DOLCETTO × FDP | 0 a | – | – | – |
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Galetto, L.; Miliordos, D.E.; Pegoraro, M.; Sacco, D.; Veratti, F.; Marzachì, C.; Bosco, D. Acquisition of Flavescence Dorée Phytoplasma by Scaphoideus titanus Ball from Different Grapevine Varieties. Int. J. Mol. Sci. 2016, 17, 1563. https://doi.org/10.3390/ijms17091563
Galetto L, Miliordos DE, Pegoraro M, Sacco D, Veratti F, Marzachì C, Bosco D. Acquisition of Flavescence Dorée Phytoplasma by Scaphoideus titanus Ball from Different Grapevine Varieties. International Journal of Molecular Sciences. 2016; 17(9):1563. https://doi.org/10.3390/ijms17091563
Chicago/Turabian StyleGaletto, Luciana, Dimitrios E. Miliordos, Mattia Pegoraro, Dario Sacco, Flavio Veratti, Cristina Marzachì, and Domenico Bosco. 2016. "Acquisition of Flavescence Dorée Phytoplasma by Scaphoideus titanus Ball from Different Grapevine Varieties" International Journal of Molecular Sciences 17, no. 9: 1563. https://doi.org/10.3390/ijms17091563