Long Non-Coding RNA Profiling in a Non-Alcoholic Fatty Liver Disease Rodent Model: New Insight into Pathogenesis
Abstract
:1. Introduction
2. Results
2.1. Animal Model of Non-Alcoholic Fatty Liver Disease
2.2. Long Non-Coding RNA Profile in Non-Alcoholic Fatty Liver Disease
2.3. Bioinformatic Analysis and Characterization of Differentially Expressed lncRNAs
2.4. Global Changes in Gene Expression Profiles of Non-Alcoholic Fatty Liver Disease and Bioinformatic Analysis
2.5. Quantitative Polymerase Chain Reaction Validation of Microarray Data
2.6. Common Pathway of Different Expressed lncRNA and Corresponding mRNA
2.7. Knockdown of lncRNA FLRL2 Increases Predicted-Target Arntl Expression and Overexpression Vice Versa
3. Discussion
4. Materials and Methods
4.1. Animal Model of Non-Alcoholic Fatty Liver Disease and Liver Samples
4.2. RNA Extraction and Purification
4.3. LncRNA and mRNA Microarray
4.4. Quantitative Polymerase Chain Reaction Validation
4.5. Cell Culture, Non-Alcoholic Fatty Liver Disease Cellular Model and Plasmid Transfection
4.6. Protein Extraction and Western Blot
4.7. Lipid Staining and Intracellular Triglyceride Quantification
4.8. Bio-Informatic Analysis of LncRNA Targets and Associated Pathways
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Chen, Y.; Huang, H.; Xu, C.; Yu, C.; Li, Y. Long Non-Coding RNA Profiling in a Non-Alcoholic Fatty Liver Disease Rodent Model: New Insight into Pathogenesis. Int. J. Mol. Sci. 2017, 18, 21. https://doi.org/10.3390/ijms18010021
Chen Y, Huang H, Xu C, Yu C, Li Y. Long Non-Coding RNA Profiling in a Non-Alcoholic Fatty Liver Disease Rodent Model: New Insight into Pathogenesis. International Journal of Molecular Sciences. 2017; 18(1):21. https://doi.org/10.3390/ijms18010021
Chicago/Turabian StyleChen, Yi, Haixiu Huang, Chengfu Xu, Chaohui Yu, and Youming Li. 2017. "Long Non-Coding RNA Profiling in a Non-Alcoholic Fatty Liver Disease Rodent Model: New Insight into Pathogenesis" International Journal of Molecular Sciences 18, no. 1: 21. https://doi.org/10.3390/ijms18010021