Genome-Wide Screen of miRNAs and Targeting mRNAs Reveals the Negatively Regulatory Effect of miR-130b-3p on PTEN by PI3K and Integrin β1 Signaling Pathways in Bladder Carcinoma
Abstract
:1. Introduction
2. Results
2.1. Differentially Expressed miRNA and mRNA Profiles Are Screened by Microarray
2.2. The Regulatory Network of miRNAs and Target Genes Are Constructed
2.3. Differently Expressed miRNAs Are Identified
2.4. Kyoto Encyclopedia of Genes and Genomes Pathway and Gene Ontology Terms Classifications Are Analyzed
2.5. Target Gene PTEN of miR-130b-3p Is Predicted and Verified
2.6. The miR-130b-3p Affects Proliferation and Apoptosis of Bladder Cancer Cells
2.7. The miR-130b-3p Promotes Invasion, Migration and Rearranges Cytoskeleton of Bladder Cancer Cells
2.8. miR-130b-3p Regulates PI3K and Integrin β1/FAK Signaling Pathways Targeting PTEN
2.9. miR-130b-3p Suppresses the Expression Target Gene PTEN
3. Discussion
4. Materials and Methods
4.1. Patient Sample
4.2. RNA Extraction and Microarray Hybridization
4.3. Microarray Data Analysis
4.4. The Network of Target Gene Predictions
4.5. qRT-PCR
4.6. GO and KEGG Enrichment Analysis
4.7. Cells, Reagents and Antibodies
4.8. Dual-Luciferase Reporter Assay
4.9. Cell Proliferation Assay
4.10. Apoptosis Detection
4.11. Wound Healing and Cell Invasion Assay
4.12. Cytoskeleton
4.13. Western Blot Analysis
4.14. HE, Immunohistochemistry and Immunofluorescence
4.15. Statistical Methods
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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UP | Fold Change | p-Value | |
---|---|---|---|
ID | Name | Exp vs. Con | Exp vs. Con |
145950 | hsa-miR-33b-5p | 3.336370154 | 0.004956079 |
146131 | hsa-miR-2117 | 2.604240009 | 0.045920679 |
168915 | hsa-miR-4780 | 2.543903736 | 0.042445646 |
17854 | hsa-miR-106b-3p | 2.535437308 | 0.034207452 |
10936 | hsa-miR-130b-3p | 2.276421905 | 0.047543441 |
42892 | hsa-miR-450b-3p | 1.995321427 | 0.023482703 |
10923 | hsa-miR-107 | 1.989390422 | 0.006583535 |
17936 | hsa-miR-16-1-3p | 1.950937554 | 0.046962876 |
42730 | hsa-miR-423-3p | 1.839347459 | 0.018558695 |
42581 | hsa-miR-513a-5p | 1.795224692 | 0.044640818 |
148327 | hsa-miR-3651 | 1.794198265 | 0.029542806 |
46869 | hsa-miR-1258 | 1.723432724 | 0.020138235 |
169167 | hsa-miR-4451 | 1.693943125 | 0.017475933 |
42949 | hsa-miR-155-3p | 1.692921273 | 0.048609104 |
17391 | hsa-miR-635 | 1.626598085 | 0.002958794 |
13179 | hsa-miR-455-5p | 1.619979204 | 0.023576850 |
145745 | hsa-miR-335-3p | 1.597940804 | 0.041387192 |
145717 | hsa-miR-516a/b-3p | 1.579328007 | 0.044895980 |
DOWN | Fold Change | p-Value | |
---|---|---|---|
ID | Name | Exp vs. Con | Exp vs. Con |
145651 | hsa-miR-99a-3p | 0.051086552 | 7.53934 × 105 |
145943 | hsa-miR-100-5p | 0.067791739 | 0.002173279 |
30787 | hsa-miR-125b-5p | 0.084582742 | 0.005679508 |
42686 | hsa-miR-136-3p | 0.102904278 | 0.016379138 |
19591 | hsa-miR-199b-5p | 0.115965971 | 0.005383998 |
29562 | hsa-miR-199a-5p | 0.128659384 | 0.004991946 |
10995 | hsa-miR-199a/b-3p | 0.143691076 | 0.005761323 |
145822 | hsa-miR-214-5p | 0.195786477 | 0.000116725 |
42829 | hsa-miR-127-3p | 0.202381027 | 0.006742221 |
10943 | hsa-miR-136-5p | 0.219393229 | 0.004850101 |
31867 | hsa-miR-145-3p | 0.222153448 | 0.023978368 |
17944 | hsa-miR-337-5p | 0.238479942 | 0.012453995 |
46626 | hsa-miR-30c-2-3p | 0.240835077 | 0.001008995 |
11018 | hsa-miR-218-5p | 0.250517237 | 0.014066474 |
17482 | hsa-miR-411-5p | 0.251814653 | 0.016992894 |
169046 | hsa-miR-4715-5p | 0.259395379 | 0.013799259 |
33596 | hsa-miR-126-5p | 0.289376368 | 0.021690880 |
169415 | hsa-miR-187-5p | 0.293809285 | 0.004695797 |
146009 | hsa-miR-376a-3p | 0.296238972 | 0.006251505 |
42629 | hsa-miR-376c-3p | 0.298543576 | 0.024114100 |
11091 | hsa-miR-377-3p | 0.311789277 | 0.009371085 |
168980 | hsa-miR-4324 | 0.313625447 | 0.023252715 |
145677 | hsa-miR-139-5p | 0.320773384 | 0.009280757 |
147673 | hsa-miR-4262 | 0.361786249 | 0.012496365 |
148039 | hsa-miR-3925-5p | 0.363658051 | 0.021581694 |
148382 | hsa-miR-3609 | 0.381682467 | 0.025550722 |
169383 | hsa-miR-548ah-5p | 0.436213703 | 0.001082679 |
42453 | hsa-miR-376b/c-5p | 0.448649921 | 0.002099911 |
168932 | hsa-miR-5094 | 0.448868287 | 0.022425735 |
169149 | hsa-miR-4659b-5p | 0.455660642 | 0.002122712 |
148089 | hsa-miR-208a-3p | 0.459113699 | 0.007047771 |
148654 | hsa-miR-184 | 0.475720561 | 0.023389351 |
11102 | hsa-miR-410-3p | 0.486892381 | 0.001975760 |
169038 | hsa-miR-488-3p | 0.496456609 | 0.004189093 |
42764 | hsa-miR-412-3p | 0.497046297 | 0.023298170 |
11104 | hsa-miR-422a | 0.507097110 | 0.021252147 |
147915 | hsa-miR-3174 | 0.514617804 | 0.013270708 |
148187 | hsa-miR-410-5p | 0.521024486 | 0.024425287 |
13130 | hsa-miR-517-5p | 0.538378530 | 0.007681208 |
168818 | hsa-miR-4774-5p | 0.544885335 | 0.020040461 |
145640 | hsa-miR-328-3p | 0.548194103 | 0.009030944 |
147537 | hsa-miR-4264 | 0.551794193 | 0.018874296 |
46623 | hsa-miR-1273a | 0.555475406 | 0.027998466 |
11111 | hsa-miR-432-5p | 0.564939359 | 0.022890766 |
145857 | hsa-miR-154-5p | 0.564947384 | 0.027411645 |
168683 | hsa-miR-4792 | 0.569054172 | 0.027386896 |
146096 | hsa-miR-764 | 0.595632201 | 0.025813179 |
168925 | hsa-miR-1273g-3p | 0.608040769 | 0.027206880 |
147776 | hsa-miR-4317 | 0.610093911 | 0.026950961 |
42673 | hsa-miR-337-3p | 0.617048724 | 0.009417660 |
17958 | hsa-miR-576-3p | 0.638884891 | 0.012748315 |
148349 | hsa-miR-3938 | 0.655804338 | 0.003662500 |
169369 | hsa-miR-4490 | 0.658510235 | 0.009235676 |
147828 | hsa-miR-3121-3p | 0.661253760 | 0.011786378 |
42729 | hsa-miR-34c-3p | 0.665477769 | 0.013533460 |
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Lv, M.; Zhong, Z.; Chi, H.; Huang, M.; Jiang, R.; Chen, J. Genome-Wide Screen of miRNAs and Targeting mRNAs Reveals the Negatively Regulatory Effect of miR-130b-3p on PTEN by PI3K and Integrin β1 Signaling Pathways in Bladder Carcinoma. Int. J. Mol. Sci. 2017, 18, 78. https://doi.org/10.3390/ijms18010078
Lv M, Zhong Z, Chi H, Huang M, Jiang R, Chen J. Genome-Wide Screen of miRNAs and Targeting mRNAs Reveals the Negatively Regulatory Effect of miR-130b-3p on PTEN by PI3K and Integrin β1 Signaling Pathways in Bladder Carcinoma. International Journal of Molecular Sciences. 2017; 18(1):78. https://doi.org/10.3390/ijms18010078
Chicago/Turabian StyleLv, Mengxin, Zhenyu Zhong, Hong Chi, Mengge Huang, Rong Jiang, and Junxia Chen. 2017. "Genome-Wide Screen of miRNAs and Targeting mRNAs Reveals the Negatively Regulatory Effect of miR-130b-3p on PTEN by PI3K and Integrin β1 Signaling Pathways in Bladder Carcinoma" International Journal of Molecular Sciences 18, no. 1: 78. https://doi.org/10.3390/ijms18010078