GhMAP3K65, a Cotton Raf-Like MAP3K Gene, Enhances Susceptibility to Pathogen Infection and Heat Stress by Negatively Modulating Growth and Development in Transgenic Nicotiana benthamiana
Abstract
:1. Introduction
2. Results
2.1. Isolation and Sequence Analysis of GhMAP3K65
2.2. GhMAP3K65 is Localized to the Cytoplasm and Nucleus
2.3. Relative Expression Profiles of GhMAP3K65
2.4. Effects of GhMAP3K65 Silencing on Pathogen Infection and Heat Stress in Cotton
2.5. Overexpression of GhMAP3K65 Enhanced Susceptibility to Pathogen Infection and Heat Stress
2.6. Overexpression of GhMAP3K65 Activated Transcription of Defence-Related Genes
2.7. Overexpression of GhMAP3K65 Impaired Ligin Biosynthesis and Stomatal Immunity in the Leaves of N. benthamiana
2.8. Overexpression of GhMAP3K65 Repressed Growth and Development of the Root System
3. Discussion
4. Materials and Methods
4.1. Plant Growth and Stress Treatments
4.2. Isolation of GhMAP3K65 Gene, Vector Construction, and Genetic Transformation
4.3. Subcellular Localization of GhMAP3K65
4.4. RNA Extraction and Quantitative PCR
4.5. Virus-Induced Gene Silencing (VIGS) of GhMAP3K65 in Cotton
4.6. Pathogen Infection
4.7. Heat Stress Treatment
4.8. Histochemical Staining
4.9. Structural Defects in Leaves
4.10. Lignin Content
4.11. Determination of Root Vitality by Triphenyl Tetrazolium Chloride (TTC) Method
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhai, N.; Jia, H.; Liu, D.; Liu, S.; Ma, M.; Guo, X.; Li, H. GhMAP3K65, a Cotton Raf-Like MAP3K Gene, Enhances Susceptibility to Pathogen Infection and Heat Stress by Negatively Modulating Growth and Development in Transgenic Nicotiana benthamiana. Int. J. Mol. Sci. 2017, 18, 2462. https://doi.org/10.3390/ijms18112462
Zhai N, Jia H, Liu D, Liu S, Ma M, Guo X, Li H. GhMAP3K65, a Cotton Raf-Like MAP3K Gene, Enhances Susceptibility to Pathogen Infection and Heat Stress by Negatively Modulating Growth and Development in Transgenic Nicotiana benthamiana. International Journal of Molecular Sciences. 2017; 18(11):2462. https://doi.org/10.3390/ijms18112462
Chicago/Turabian StyleZhai, Na, Haihong Jia, Dongdong Liu, Shuchang Liu, Manli Ma, Xingqi Guo, and Han Li. 2017. "GhMAP3K65, a Cotton Raf-Like MAP3K Gene, Enhances Susceptibility to Pathogen Infection and Heat Stress by Negatively Modulating Growth and Development in Transgenic Nicotiana benthamiana" International Journal of Molecular Sciences 18, no. 11: 2462. https://doi.org/10.3390/ijms18112462