Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells
Abstract
:1. Introduction
2. Results
2.1. The Positive Correlation of Prp19 and Cdc5L in HCC
2.2. Prp19 Binds with Cdc5L and Modulates Cdc5L Expression in HCC Cells
2.3. Prp19 Modulates Cdc5L Expression Via Inhibiting mRNA Translation and Facilitating Lysosome-Mediated Degradation in HCC Cells
2.4. Prp19 Regulates Mitotic Progression via Cdc5L in HCC Cells
3. Discussion
4. Materials and Methods
4.1. Patients and Samples
4.2. Immunochemistry and Immunofluorescence
4.3. Cell Lines, Plasmids, siRNAs, Reagents and Chemical Agents
4.4. Real-Time PCR (qPCR)
4.5. Western Blot Assay
4.6. Immunoprecipitation Analysis
4.7. Dual Luciferase Analysis
4.8. Flow Cytometric Analysis
4.9. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Huang, R.; Xue, R.; Qu, D.; Yin, J.; Shen, X.-Z. Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells. Int. J. Mol. Sci. 2017, 18, 778. https://doi.org/10.3390/ijms18040778
Huang R, Xue R, Qu D, Yin J, Shen X-Z. Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells. International Journal of Molecular Sciences. 2017; 18(4):778. https://doi.org/10.3390/ijms18040778
Chicago/Turabian StyleHuang, Renzheng, Ruyi Xue, Di Qu, Jie Yin, and Xi-Zhong Shen. 2017. "Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells" International Journal of Molecular Sciences 18, no. 4: 778. https://doi.org/10.3390/ijms18040778