Folic Acid Reduces Tau Phosphorylation by Regulating PP2A Methylation in Streptozotocin-Induced Diabetic Mice
Abstract
:1. Introduction
2. Results
2.1. Serum Folate and Blood Glucose
2.2. Effect of Folic Acid on TAU Phosphorylation in DM Mice
2.3. Effect of Folic Acid on GSK-3β Phosphorylation
2.4. Effect of Folic Acid on PP2A Methylation
2.5. Methylation Potential
2.6. Effect of Folic Acid on DNMT mRNA Expression
3. Discussion
4. Materials and Methods
4.1. Animals and STZ Treatment
4.2. Experimental Diets
4.3. Serum Folate, Blood Glucose, and Plasma Hcy Levels
4.4. Methylation Potential Assay
4.5. Real-Time PCR
4.6. Western Blotting
4.7. Immunohistochemistry
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
AD | Alzheimer’s disease |
STZ | streptozotocin |
SAM | S-adenosyl methionine |
SAH | S-adenosyl homocysteine |
DNMT | DNA methyltransferase |
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Zheng, M.; Zou, C.; Li, M.; Huang, G.; Gao, Y.; Liu, H. Folic Acid Reduces Tau Phosphorylation by Regulating PP2A Methylation in Streptozotocin-Induced Diabetic Mice. Int. J. Mol. Sci. 2017, 18, 861. https://doi.org/10.3390/ijms18040861
Zheng M, Zou C, Li M, Huang G, Gao Y, Liu H. Folic Acid Reduces Tau Phosphorylation by Regulating PP2A Methylation in Streptozotocin-Induced Diabetic Mice. International Journal of Molecular Sciences. 2017; 18(4):861. https://doi.org/10.3390/ijms18040861
Chicago/Turabian StyleZheng, Miaoyan, Chen Zou, Mengyue Li, Guowei Huang, Yuxia Gao, and Huan Liu. 2017. "Folic Acid Reduces Tau Phosphorylation by Regulating PP2A Methylation in Streptozotocin-Induced Diabetic Mice" International Journal of Molecular Sciences 18, no. 4: 861. https://doi.org/10.3390/ijms18040861