ρ0 Cells Feature De-Ubiquitination of SLC Transporters and Increased Levels and Fluxes of Amino Acids
Abstract
:1. Introduction
2. Results and Discussion
2.1. Amino Acid Flux of ρ0 and 143B.TK- Cells
2.2. Determination of Absolute Amino Acid Concentrations
2.3. Solute Carrier Transporter Inhibition
3. Methods
3.1. Cell Culture
3.2. Amino Acid Flux Assay
3.3. SLC Transporter Inhibitor Assay
3.4. Metabolite Extraction and Profiling by Targeted Liquid Chromatography Mass Spectrometry (LC-MS)
3.5. Statistical Analyses
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
ATP | Adenosine triphosphate |
LC-MS | Liquid chromatography mass spectrometry |
MRM | Multiple reaction monitoring |
SILAC | Stable isotope labeling with amino acids in cell culture |
SLC | Solute carrier |
PTM | Post-translational modification |
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Medina, A.B.; Banaszczak, M.; Ni, Y.; Aretz, I.; Meierhofer, D. ρ0 Cells Feature De-Ubiquitination of SLC Transporters and Increased Levels and Fluxes of Amino Acids. Int. J. Mol. Sci. 2017, 18, 879. https://doi.org/10.3390/ijms18040879
Medina AB, Banaszczak M, Ni Y, Aretz I, Meierhofer D. ρ0 Cells Feature De-Ubiquitination of SLC Transporters and Increased Levels and Fluxes of Amino Acids. International Journal of Molecular Sciences. 2017; 18(4):879. https://doi.org/10.3390/ijms18040879
Chicago/Turabian StyleMedina, André Bordinassi, Marcin Banaszczak, Yang Ni, Ina Aretz, and David Meierhofer. 2017. "ρ0 Cells Feature De-Ubiquitination of SLC Transporters and Increased Levels and Fluxes of Amino Acids" International Journal of Molecular Sciences 18, no. 4: 879. https://doi.org/10.3390/ijms18040879