The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity
Abstract
:1. Introduction
2. Results
2.1. Role of 6-Hydroxydopamine (6-OHDA) in Decreased Cell Viability of SH-SY5Y Cells
2.2. Effect of 1T3O on 6-OHDA-Induced SH-SY5Y Neuroblastoma Apoptosis
2.3. Effect of 1T3O on the Akt (Phospho-Akt), Phospho-Extracellular Signal-Regulated Kinase (Phospho-ERK), and Phospho-p38 Protein Expression in SH-SY5Y Cells
2.4. Effect of 1T3O on 6-OHDA-Induced Activation of Cellular Caspase-3
2.5. Effect of 1T3O on 6-OHD-Induced Increase in Oxidative Stress in SH-SY5Y Cells
2.6. Effect of 1T3O on 6-OHDA-Induced Changes in Proteins Related to the Akt/Nrf2 Signaling Pathway of SH-SY5Y Neuroblastomas
2.7. Attenuation of the Neuroprotective Effect of 1T3O by LY294002 and Zinc Protoporphyrin (ZnPP)
2.8. Effect of 1T3O on Zebrafish Mortality
2.9. Effect of 1T3O on 6-OHDA-Induced Deficits in Zebrafish Locomotor Activity
2.10. Effect of 1T3O on 6-OHDA-Induced Changes in the Expression Levels of TNF-α and TH in Zebrafish Brain Tissue
3. Discussion
3.1. Compounds Conferring Neuroprotection via the Nrf/HO-1 Pathway
3.2. Relevant Compounds Inhibiting the p38 Pathway and Activating the ERK Pathway
3.3. Inhibiting 6-OHDA-Induced SH-SY5Y Apoptosis via the Akt Pathway
3.4. Analysis of the Neuroprotective Effect of the Compound in a Zebrafish PD Model
3.5. Neuroprotective Mechanism of the Compound in the Zebrafish PD Model
4. Materials and Methods
4.1. Preparation of Cell
4.2. Neuroprotective Analysis
4.3. Cell Morphology
4.4. Analysis of Cellular Oxidative Stress (CellROX® Stain)
4.5. Hoechst Stain
4.6. TUNEL Stain
4.7. Oxidative Stress (SOD Activity Assay)
4.8. Fish Maintenance
4.9. Locomotor Behavioral Test
4.10. Western Blot Analysis
4.10.1. Protein Extraction from Cultured Cells
4.10.2. Preparation of Nuclear Extracts
4.10.3. Protein Extraction from Zebrafish
4.10.4. Western Blotting
4.11. Data Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
PD | Parkinson’s disease |
ND | neurodegenerative disease |
6-OHDA | 6-hydroxydopamine |
1T3O | 1-tosylpentan-3-one |
TNF-α | tumor necrosis factor-alpha |
cAMP | cyclic adenosine monophosphate |
CRE | cAMP responsive element |
CREB | cAMP responsive element binding protein |
PI3K | phosphoinositide 3-kinase |
Akt | protein kinase B |
ERK | extracellular signal–regulated kinase |
MAPK | mitogen-activated protein kinase |
Nrf2 | nuclear factor erythroid 2–related factor 2 |
HO-1 | heme oxygenase-1 |
TUNEL | terminal deoxynucleotidyl transferase dUTP nick end labeling |
Bcl-xL | B-cell lymphoma-extra-large |
d-UTP | 2´-deoxyuridine 5´-triphosphate |
DAPI | 4′,6-diamidino-2-phenylindole |
SOD | superoxidase dismutase |
ZnPP | zinc protoporphyrin |
ARE | antioxidant response element |
NQO1 | NAD(P)H:quinone-oxidoreductase-1 |
NAD(P)H | lactaldehyde reductase |
GSHP | glutathione peroxidase |
PGE2 | prostaglandin E2 |
WST-1 | Water-soluble tetrazolium salts-1 |
HRP | horseradish peroxidase |
MAO-B | monoamine oxidase B |
EGCG | epigallocatechin gallate |
MPP+ | 1-methyl-4-phenylpyridinium |
MPTP | 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine |
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Concentration (μM) | Mortality Count | Mortality (%) |
---|---|---|
Control | 0/24 | 0 |
100 | 24/24 | 100 |
50 | 0/24 | 0 |
5 | 0/24 | 0 |
5 × 10−1 | 0/24 | 0 |
5 × 10−2 | 0/24 | 0 |
5 × 10−3 | 0/24 | 0 |
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Kao, C.-J.; Chen, W.-F.; Guo, B.-L.; Feng, C.-W.; Hung, H.-C.; Yang, W.-Y.; Sung, C.-S.; Tsui, K.-H.; Chu, H.; Chen, N.-F.; et al. The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity. Int. J. Mol. Sci. 2017, 18, 1096. https://doi.org/10.3390/ijms18051096
Kao C-J, Chen W-F, Guo B-L, Feng C-W, Hung H-C, Yang W-Y, Sung C-S, Tsui K-H, Chu H, Chen N-F, et al. The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity. International Journal of Molecular Sciences. 2017; 18(5):1096. https://doi.org/10.3390/ijms18051096
Chicago/Turabian StyleKao, Chien-Jen, Wu-Fu Chen, Bo-Lin Guo, Chien-Wei Feng, Han-Chun Hung, Wen-Ya Yang, Chun-Sung Sung, Kuan-Hao Tsui, Hsin Chu, Nan-Fu Chen, and et al. 2017. "The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity" International Journal of Molecular Sciences 18, no. 5: 1096. https://doi.org/10.3390/ijms18051096
APA StyleKao, C. -J., Chen, W. -F., Guo, B. -L., Feng, C. -W., Hung, H. -C., Yang, W. -Y., Sung, C. -S., Tsui, K. -H., Chu, H., Chen, N. -F., & Wen, Z. -H. (2017). The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity. International Journal of Molecular Sciences, 18(5), 1096. https://doi.org/10.3390/ijms18051096