Cotton Ascorbate Oxidase Promotes Cell Growth in Cultured Tobacco Bright Yellow-2 Cells through Generation of Apoplast Oxidation
Abstract
:1. Introduction
2. Results
2.1. Identification of Cotton Ascorbate Oxidase
2.2. Cotton (Gossypium hirsutum) Ascorbate Oxidase Gene (GhAO1) Is Preferentially Expressed during the Fiber Cell Fast Elongation Period at the mRNA Level and Total Protein Activity
2.3. GhAO1 Is a Cell Wall Protein
2.4. Overexpression of GhAO1 Promotes Cell Growth in Tobacco Bright Yellow-2 (BY-2) Cells
2.5. DHA Is Enriched in GhAO1-Overexpressing Tobacco BY-2 Cells
2.6. Ascorbate Oxidase (AO) Induces H2O2 Accumulation
2.7. AO Affects Expression of Ca2+ Channel Genes
2.8. AO Expression Is Modulated by Auxin
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Functional Sequence Analysis
4.3. Vector Construction
4.4. RNA Extraction and Quantitative Real-Time Polymerase Chain Reaction (QRT-PCR)
4.5. Subcellular Localization Analysis
4.6. Isolation of Apoplastic Fluid
4.7. Ascorbate Determination
4.8. Assays of Enzyme Activities
4.9. Transformation of BY-2 Cells
4.10. Observation of BY-2 Cells
4.11. Treatment of Tobacco BY-2 Cells
4.12. H2O2 Measurement
4.13. Accession Numbers
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Li, R.; Xin, S.; Tao, C.; Jin, X.; Li, H. Cotton Ascorbate Oxidase Promotes Cell Growth in Cultured Tobacco Bright Yellow-2 Cells through Generation of Apoplast Oxidation. Int. J. Mol. Sci. 2017, 18, 1346. https://doi.org/10.3390/ijms18071346
Li R, Xin S, Tao C, Jin X, Li H. Cotton Ascorbate Oxidase Promotes Cell Growth in Cultured Tobacco Bright Yellow-2 Cells through Generation of Apoplast Oxidation. International Journal of Molecular Sciences. 2017; 18(7):1346. https://doi.org/10.3390/ijms18071346
Chicago/Turabian StyleLi, Rong, Shan Xin, Chengcheng Tao, Xiang Jin, and Hongbin Li. 2017. "Cotton Ascorbate Oxidase Promotes Cell Growth in Cultured Tobacco Bright Yellow-2 Cells through Generation of Apoplast Oxidation" International Journal of Molecular Sciences 18, no. 7: 1346. https://doi.org/10.3390/ijms18071346