The Emerging Role of Zfp217 in Adipogenesis
Abstract
:1. Introduction
2. Results
2.1. The Potential Adipogenesis Role of Zfp217 Based on Gene Expression Omnibus (GEO) Datasets
2.2. The Association of Zfp217 Expression with Adipogenesis and Obesity
2.3. Zfp217 Regulates Adipogenesis of C3H10T1/2 and 3T3-L1
2.4. Zfp217 Positively Regulates Adipogenesis by Suppressing Cell Proliferation and Interacting with Ezh2
2.5. Direct Targeting of Zfp217 by miR-503-5p, miR-135a-5p and miR-19a-3p Impairs Adipocyte Differentiation
3. Discussion
4. Materials and Methods
4.1. Analysis of Gene Expression Omnibus Series (GSE) Data
4.2. Animals
4.3. Cell Culture and Adipocyte Differentiation
4.4. Oil Red O Staining
4.5. Triglyceride Assay
4.6. Quantitative Real-Time RT-PCR (qRT-PCR) Analysis
4.7. Western Blotting
4.8. EdU Cell Proliferation Assay
4.9. Mammalian Two-Hybrid Assay
4.10. The Prediction and Screening of miRNAs
4.11. Dual-Luciferase Reporter Assay
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
Zfp217 | Zinc Finger Protein 217 |
Pparg | Peroxisome Proliferator-Activated Receptor g |
Cebps | CCAAT/Enhancer-Binding Proteins |
DEGs | Differentially Expressed Genes |
ASVC | Adipocytic Differentiation of Porcine Subcutaneous Stromal Vascular Cells |
GEO | Gene Expression Omnibus |
GSE | Gene Expression Omnibus Series |
GPL | Gene Expression Omnibus Platform |
DM | Diabetes Mellitus |
IGT | Impaired Glucose Tolerance |
NGT | Normal Glucose Tolerance |
HFD | High Fat Diet Fed Mice |
NCD | Normal Chow Diet Fed Mice |
ORF | Open Reading Frame |
EdU | 5-Ethynyl-2’-Deoxyuridine |
TG | Triglycerides |
BCA | Bicinchoninic Acid |
wt | Wild Type |
mut | Mutant Type |
hsa | Homo sapiens (human) |
mmu | Mus musculus (Mouse) |
WAT | White Adipose Tissue |
BAT | Brown Adipose Tissue |
PVDF | Polyvinylidene Difluoride |
EMT | Epithelial-to-Mesenchymal Transition |
SBGS | Simpson-Golabi-Behmel Syndrome |
GO | Gene Ontology |
KM | Kun Ming |
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Xiang, H.; Zhong, Z.-X.; Peng, Y.-D.; Jiang, S.-W. The Emerging Role of Zfp217 in Adipogenesis. Int. J. Mol. Sci. 2017, 18, 1367. https://doi.org/10.3390/ijms18071367
Xiang H, Zhong Z-X, Peng Y-D, Jiang S-W. The Emerging Role of Zfp217 in Adipogenesis. International Journal of Molecular Sciences. 2017; 18(7):1367. https://doi.org/10.3390/ijms18071367
Chicago/Turabian StyleXiang, Hong, Zhu-Xia Zhong, Yong-Dong Peng, and Si-Wen Jiang. 2017. "The Emerging Role of Zfp217 in Adipogenesis" International Journal of Molecular Sciences 18, no. 7: 1367. https://doi.org/10.3390/ijms18071367